E.C. Segar
Born	(1894-12-08)December 8, 1894 Chester, Illinois
Died	October 13, 1938(1938-10-13) (aged 43) Santa Monica, California
Nationality	American
Area(s)	cartoonist
Notable works	Popeye

Elzie Crisler Segar (December 8, 1894 – October 13, 1938) was an American cartoonist, best known as the creator of Popeye, a character who first appeared in 1929 in his comic strip Thimble Theatre. Asked how to say his name, he told The Literary Digest it was "SEE-gar".^[1] He commonly signed his work simply Segar or E. Segar above a drawing of a cigar.

History[link]

Segar was born and raised in Chester, Illinois, a small town near the Mississippi River. The son of a handyman, his earliest work experiences included assisting his father in house painting and paper hanging. Skilled at playing drums, he also provided musical accompaniment to films and vaudeville acts in the local theater, where he was eventually given the job of film projectionist. At age 18, he decided to become a cartoonist. He took a correspondence course in cartooning from W.L. Evans of Cleveland, Ohio.^[2] He said that after work he "lit up the oil lamps about midnight and worked on the course until 3 a.m."

Segar moved to Chicago where he met Richard F. Outcault, creator of The Yellow Kid and Buster Brown. Outcault encouraged him and introduced him at the Chicago Herald. On March 12, 1916, the Herald published Segar's first comic, Charlie Chaplin's Comedy Capers, which ran for a little over a year. In 1918, he moved on to William Randolph Hearst's Chicago Evening American where he created Looping the Loop. Segar married Myrtle Johnson that year; they had two children.

[edit] Popeye

Evening American Managing editor William Curley thought Segar could succeed in New York, so he sent him to King Features Syndicate, where Segar worked for many years. He began by drawing Thimble Theatre for the New York Journal. The strip made its debut on December 19, 1919, featuring the characters Olive Oyl, Castor Oyl and Horace Hamgravy, whose name was quickly shortened in the strip to simply "Ham Gravy". They were the strip's leads for about a decade. In January 1929, when Castor Oyl needed a mariner to navigate his ship to Dice Island, Castor picked up an old salt down by the docks named Popeye. Popeye's first line in the strip, upon being asked if he was a sailor, was "'Ja think I'm a cowboy?" The character stole the show and became the permanent star. Some of the other notable characters Segar created include J. Wellington Wimpy and Eugene the Jeep.

[edit] The Five-Fifteen/Sappo

Segar also created The Five-Fifteen for King Features in 1920; it was retitled Sappo in 1926. Sappo ran as a topper to the Thimble Theatre Sunday pages.

Legacy and reprints[link]

After prolonged illness, Segar died of leukemia and liver disease at the age of 43.^[3] Segar is widely regarded as one of the most influential and talented cartoonists of all time, among the first to combine humor with long-running adventures. A revival of interest in Segar's creations began with Woody Gelman's Nostalgia Press. Robert Altman's live-action film Popeye (1980) is adapted from E. C. Segar's Thimble Theatre comic strip. The screenplay by Jules Feiffer was based directly on Gelman's Thimble Theatre Starring Popeye the Sailor, a hardcover reprint collection of 1936-37 Segar strips published in 1971 by Nostalgia Press.^[4] In 2006, Fantagraphics published the first of a planned six-volume book set reprinting all Thimble Theatre daily and Sunday strips from 1928–38, beginning with the adventure that introduced Popeye.

In 1971, the National Cartoonists Society created the Elzie Segar Award in his honor. According to the Society's website, the award was "presented to a person who has made a unique and outstanding contribution to the profession of cartooning." The NCS board of directors chose the first winners, while King Features selected recipients in later years. Honorees have included Charles Schulz, Bil Keane, Al Capp, Bill Gallo and Mort Walker. The award was discontinued in 1999.^[5]

In 2012, writer Roger Langridge and cartoonist Bruce Ozella teamed to revive the spirit of Segar in their four-issue miniseries, Popeye, published by IDW.

Popeye Picnic[link]

In 1977, Segar's hometown of Chester, Illinois honored its native son with a park named in his honor. The park is home to a six-foot-tall bronze statue of Popeye, and since 1980 has been the site of the annual Popeye Picnic, a weekend-long event that celebrates the character with a parade, film festival and other activities.^[6] In 2006, Chester launched the ambitious "Popeye & Friends Character Trail," which links a series of statues of Segar's characters located throughout town.^[7] Each stands on a base inscribed with the names of donors who contributed to its cost and is unveiled and dedicated during the Popeye Picnic. The 2006 debut sculpture of hamburger-loving Wimpy stands in Gazebo Park. A statue of Olive Oyl, Swee'Pea and the Jeep, located downtown near the Randolph County Courthouse, followed in 2007. In 2008, a Bluto statue was dedicated at the corner of Swanwick and W. Holmes Streets, in front of Buena Vista Bank. The 2009 statue of Castor Oyl and Bernice the Whiffle Hen stands in front of Chester Memorial Hospital. An additional 11 statues will be unveiled at the rate of one per year until 2019, when a bust of Segar at his birthplace will mark the cartoonist's 125th birthday. To keep the slate on schedule, one year will feature two dedications, with the Sea Hag as the "wild card." According to the "Map to the Stars" promo piece released by the town, the schedule continues as follows:

A few Chester businesses are named for Popeye characters, including Rough-House Pizza and Sweet Pea's [sic] Restaurant (renamed under new management).^[8]

On December 8, 2009, Google honored Segar's 115th birthday with a Google Doodle of Popeye. The doodle used Popeye's body as the 'g', had 'oogl' drawn to resemble Segar's drawing style, and a spinach can as the 'e'; and featured Popeye punching the 'oogl' to get the spinach to fly at him through the air.

References[link]

External links[link]

• "E.C. Segar" by Ed Black
• "The Rime of the Ancient Mariner" by Zak Sally Minneapolis City Pages
• E.C. Segar
• E.C. Segar Gallery
• "Popeye's Pop EC Segar"
• E.C. Segar's 115th Birthday Doodle in Google Logo Museum
• E. C. Segar at Find a Grave

Escherichia coli
Scientific classification
Domain:	Bacteria
Kingdom:	Eubacteria
Phylum:	Proteobacteria
Class:	Gammaproteobacteria
Order:	Enterobacteriales
Family:	Enterobacteriaceae
Genus:	Escherichia
Species:	E. coli
Binomial name
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Synonyms
Bacillus coli communis Escherich 1885

Escherichia coli ( /ˌɛʃɨˈrɪkiə ˈkoʊlaɪ/;^[1] commonly abbreviated E. coli) is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans, and are occasionally responsible for product recalls due to food contamination.^[2][3] The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K₂,^[4] and by preventing the establishment of pathogenic bacteria within the intestine.^[5][6]

E. coli and related bacteria constitute about 0.1% of gut flora,^[7] and fecal-oral transmission is the major route through which pathogenic strains of the bacterium cause disease. Cells are able to survive outside the body for a limited amount of time, which makes them ideal indicator organisms to test environmental samples for fecal contamination.^[8][9] There is, however, a growing body of research that has examined environmentally persistent E. coli which can survive for extended periods of time outside of the host.^[10] The bacterium can also be grown easily and inexpensively in a laboratory setting, and has been intensively investigated for over 60 years. E. coli is the most widely studied prokaryotic model organism,^{[citation needed]} and an important species in the fields of biotechnology and microbiology, where it has served as the host organism for the majority of work with recombinant DNA.

History[link]

The genera Escherichia and Salmonella diverged around 102 million years ago (credibility interval: 57–176 mya), which coincides with the divergence of their hosts: the former being found in mammals and the latter in birds and reptiles.^[11] This was followed by a split of the escherichian ancestor into five species (E. albertii, E. coli, E. fergusonii, E. hermannii and E. vulneris. The last E. coli ancestor split between 20 and 30 mya.^[12]

In 1885, Theodor Escherich, a German pediatrician, first discovered this species in the feces of healthy individuals and called it Bacterium coli commune due to the fact it is found in the colon and early classifications of Prokaryotes placed these in a handful of genera based on their shape and motility (at that time Ernst Haeckel's classification of Bacteria in the kingdom Monera was in place^[13]).^[14] Bacterium coli was the type species of the now invalid genus Bacterium when it was revealed that the former type species ("Bacterium triloculare") was missing.^[15] Following a revision of Bacteria it was reclassified as Bacillus coli by Migula in 1895^[16] and later reclassified in the newly created genus Escherichia, named after its original discoverer.^[17]

The genus belongs in a group of bacteria informally known as "coliforms", and is a member of the Enterobacteriaceae family ("the enterics") of the Gammaproteobacteria.^[18]

In May 2011, one E. coli strain, Escherichia coli O104:H4, has been the subject of a bacterial outbreak that began in Germany. Certain strains of E. coli are a major cause of foodborne illness. The outbreak started when several people in Germany were infected with enterohemorrhagic E. coli (EHEC) bacteria, leading to hemolytic-uremic syndrome (HUS), a medical emergency that requires urgent treatment. The outbreak did not only concern Germany, but 11 other countries, including regions in North America. ^[19] On 30 June 2011 the German Bundesinstitut für Risikobewertung (BfR) (Federal Institute for Risk Assessment, a federal, fully legal entity under public law of the Federal Republic of Germany, an institute within the German Federal Ministry of Food, Agriculture and Consumer Protection) announced that seeds of fenugreek from Egypt were likely the cause of the EHEC outbreak.^[20]

Biology and biochemistry[link]

Model of successive binary fission in E. coli

E. coli is Gram-negative, facultative anaerobic and non-sporulating. Cells are typically rod-shaped, and are about 2.0 microns (μm) long and 0.5 μm in diameter, with a cell volume of 0.6 – 0.7 (μm)³.^[21][22] It can live on a wide variety of substrates. E. coli uses mixed-acid fermentation in anaerobic conditions, producing lactate, succinate, ethanol, acetate and carbon dioxide. Since many pathways in mixed-acid fermentation produce hydrogen gas, these pathways require the levels of hydrogen to be low, as is the case when E. coli lives together with hydrogen-consuming organisms, such as methanogens or sulphate-reducing bacteria.^[23]

Optimal growth of E. coli occurs at 37°C (98.6°F) but some laboratory strains can multiply at temperatures of up to 49°C (120.2°F).^[24] Growth can be driven by aerobic or anaerobic respiration, using a large variety of redox pairs, including the oxidation of pyruvic acid, formic acid, hydrogen and amino acids, and the reduction of substrates such as oxygen, nitrate, fumarate, dimethyl sulfoxide and trimethylamine N-oxide.^[25]

Strains that possess flagella are motile. The flagella have a peritrichous arrangement.^[26]

E. coli and related bacteria possess the ability to transfer DNA via bacterial conjugation, transduction or transformation, which allows genetic material to spread horizontally through an existing population. This process led to the spread of the gene encoding shiga toxin from Shigella to E. coli O157:H7, carried by a bacteriophage.^[27]

Diversity[link]

Escherichia coli encompasses an enormous population of bacteria that exhibit a very high degree of both genetic and phenotypic diversity. Genome sequencing of a large number of isolates of E. coli and related bacteria shows that a taxonomic reclassification would be desirable. However, this has not been done, largely due to its medical importance^[28] and Escherichia coli remains one of the most diverse bacterial species: only 20% of the genome is common to all strains.^[29] In fact, from the evolutionary point of view, the members of genus Shigella (dysenteriae, flexneri, boydii, sonnei) should be classified as E. coli strains, a phenomenon termed taxa in disguise.^[30] Similarly, other strains of E. coli (e.g. the K-12 strain commonly used in recombinant DNA work) are sufficiently different that they would merit reclassification.

A strain is a sub-group within the species that has unique characteristics that distinguish it from other strains. These differences are often detectable only at the molecular level; however, they may result in changes to the physiology or lifecycle of the bacterium. For example, a strain may gain pathogenic capacity, the ability to use a unique carbon source, the ability to take upon a particular ecological niche or the ability to resist antimicrobial agents. Different strains of E. coli are often host-specific, making it possible to determine the source of fecal contamination in environmental samples.^[8][9] For example, knowing which E. coli strains are present in a water sample allows researchers to make assumptions about whether the contamination originated from a human, another mammal or a bird.

Serotypes[link]

A common subdivision system of E. coli, but not based on evolutionary relatedness, is by serotype, which is based on major surface antigens (O antigen: part of lipopolysaccharide layer; H: flagellin; K antigen: capsule), e.g. O157:H7)^[31] (NB: K-12, the common laboratory strain is not a serotype.)

Genome plasticity[link]

Like all lifeforms, new strains of E. coli evolve through the natural biological processes of mutation, gene duplication and horizontal gene transfer, in particular 18% of the genome of the laboratory strain MG1655 was horizontally acquired since the divergence from Salmonella.^[32] In microbiology, all strains of E. coli derive from E. coli K-12 or E. coli B strains. Some strains develop traits that can be harmful to a host animal. These virulent strains typically cause a bout of diarrhea that is unpleasant in healthy adults and is often lethal to children in the developing world.^[33] More virulent strains, such as O157:H7 cause serious illness or death in the elderly, the very young or the immunocompromised.^[5][33]

Neotype strain[link]

E. coli is the type species of the genus (Escherichia) and in turn Escherichia is the type genus of the family Enterobacteriaceae, where it should be noted that the family name does not stem from the genus Enterobacter + "i" (sic.) + "aceae", but from "enterobacterium" + "aceae" (enterobacterium being not a genus, but an alternative trivial name to enteric bacterium).^[18][34][35]

The original strain described by Escherich is believed to be lost, consequently a new type strain (neotype) was chosen as a representative: the neotype strain is ATCC 11775, also known as NCTC 9001,^[36] which is pathogenic to chickens and has a O1:K1:H7 serotype.^[37] However, in most studies either O157:H7 or K-12 MG1655 or K-12 W3110 are used as a representative E.coli.

[edit] Phylogeny of Escherichia coli strains

Escherichia coli is a species. A large number of strains belonging to this species have been isolated and characterised. In addition to serotype (vide supra), they can be classified according to their phylogeny, i.e. the inferred evolutionary history, as shown below where the species is divided into six groups.^[29][38][39]

The link between phylogenetic distance ("relatedness") and pathology is small, e.g. the O157:H7 serotype strains, which form a clade ("an exclusive group") — group E below — are all enterohaerogic strains (EHEC), but not all EHEC strains are closely related. In fact, four different species of Shigella are nested among E. coli strains (vide supra), while Escherichia albertii and Escherichia fergusonii are outside of this group. All commonly used research strains of E. coli' belong to group A and are derived mainly from Clifton's K-12 strain (λ⁺ F⁺; O16) and to a lesser degree from d'Herelle's "Bacillus coli" strain (B strain)(O7).

Genomes[link]

The first complete DNA sequence of an E. coli genome (laboratory strain K-12 derivative MG1655) was published in 1997. It was found to be a circular DNA molecule 4.6 million base pairs in length, containing 4288 annotated protein-coding genes (organized into 2584 operons), seven ribosomal RNA (rRNA) operons, and 86 transfer RNA (tRNA) genes. Despite having been the subject of intensive genetic analysis for approximately 40 years, a large number of these genes were previously unknown. The coding density was found to be very high, with a mean distance between genes of only 118 base pairs. The genome was observed to contain a significant number of transposable genetic elements, repeat elements, cryptic prophages, and bacteriophage remnants.^[40]

Today, over 60 complete genomic sequences of Escherichia and Shigella species are available. Comparison of these sequences shows a remarkable amount of diversity; only about 20% of each genome represents sequences that are present in every one of the isolates, while approximately 80% of each genome can vary among isolates.^[29] Each individual genome contains between 4,000 and 5,500 genes, but the total number of different genes among all of the sequenced E. coli strains (the pan-genome) exceeds 16,000. This very large variety of component genes has been interpreted to mean that two-thirds of the E. coli pan-genome originated in other species and arrived through the process of horizontal gene transfer.^[41]

Role as normal microbiota[link]

E. coli normally colonizes an infant's gastrointestinal tract within 40 hours of birth, arriving with food or water or with the individuals handling the child. In the bowel, it adheres to the mucus of the large intestine. It is the primary facultative anaerobe of the human gastrointestinal tract.^[42] (Facultative anaerobes are organisms that can grow in either the presence or absence of oxygen.) As long as these bacteria do not acquire genetic elements encoding for virulence factors, they remain benign commensals.^[43]

[edit] Therapeutic use of nonpathogenic E. coli

According to its manufacturer, nonpathogenic Escherichia coli strain Nissle 1917 also known as Mutaflor is used as a probiotic agent in medicine, mainly for the treatment of various gastroenterological diseases,^[44] including inflammatory bowel disease.^[45]

Role in disease[link]

This section requires expansion.

Virulent strains of E. coli can cause gastroenteritis, urinary tract infections, and neonatal meningitis. In rarer cases, virulent strains are also responsible for hemolytic-uremic syndrome, peritonitis, mastitis, septicemia and Gram-negative pneumonia.^[42]

Model organism in life science research[link]

Role in biotechnology[link]

Because of its long history of laboratory culture and ease of manipulation, E. coli also plays an important role in modern biological engineering and industrial microbiology.^[46] The work of Stanley Norman Cohen and Herbert Boyer in E. coli, using plasmids and restriction enzymes to create recombinant DNA, became a foundation of biotechnology.^[47]

E. coli is a very versatile host for the production of heterologous proteins,^[48] and various protein expression systems have been developed which allow the production of recombinant proteins in E. coli. Researchers can introduce genes into the microbes using plasmids which permit high level expression of protein, and such protein may be mass produced in industrial fermentation processes. One of the first useful applications of recombinant DNA technology was the manipulation of E. coli to produce human insulin.^[49] Many proteins previously thought difficult or impossible to be expressed in E. coli in folded form have also been successfully expressed in E. coli. For example, proteins with multiple disulphide bonds may be produced in the periplasmic space or in the cytoplasm of mutants rendered sufficiently oxidizing to allow disulphide-bonds to form,^[50] while proteins requiring post-translational modification such as glycosylation for stability or function have been expressed using the N-linked glycosylation system of Campylobacter jejuni engineered into E. coli.^[51][52][53]

Modified E. coli cells have been used in vaccine development, bioremediation, and production of immobilised enzymes.^[48]

Model organism[link]

E. coli is frequently used as a model organism in microbiology studies. Cultivated strains (e.g. E. coli K12) are well-adapted to the laboratory environment, and, unlike wild type strains, have lost their ability to thrive in the intestine. Many lab strains lose their ability to form biofilms.^[54][55] These features protect wild type strains from antibodies and other chemical attacks, but require a large expenditure of energy and material resources.

In 1946, Joshua Lederberg and Edward Tatum first described the phenomenon known as bacterial conjugation using E. coli as a model bacterium,^[56] and it remains the primary model to study conjugation.^{[citation needed]} E. coli was an integral part of the first experiments to understand phage genetics,^[57] and early researchers, such as Seymour Benzer, used E. coli and phage T4 to understand the topography of gene structure.^[58] Prior to Benzer's research, it was not known whether the gene was a linear structure, or if it had a branching pattern.

E. coli was one of the first organisms to have its genome sequenced; the complete genome of E. coli K12 was published by Science in 1997.^[59]

The long-term evolution experiments using E. coli, begun by Richard Lenski in 1988, have allowed direct observation of major evolutionary shifts in the laboratory.^[60] In this experiment, one population of E. coli unexpectedly evolved the ability to aerobically metabolize citrate, which is extremely rare in E. coli. As the inability to grow aerobically is normally used as a diagnostic criterion with which to differentiate E. coli from other, closely related bacteria, such as Salmonella, this innovation may mark a speciation event observed in the lab.

By evaluating the possible combination of nanotechnologies with landscape ecology, complex habitat landscapes can be generated with details at the nanoscale.^[61] On such synthetic ecosystems, evolutionary experiments with E. coli have been performed to study the spatial biophysics of adaptation in an island biogeography on-chip.

Studies are also being performed into programming E. coli to potentially solve complicated mathematics problems, such as the Hamiltonian path problem.^[62]

See also[link]

• Bacteriological water analysis
• Coliform bacteria
• Contamination control
• Dam dcm strain
• Fecal coliforms
• GIANTmicrobes
• International Code of Nomenclature of Bacteria
• List of bacterial genera named after personal names
• Mannan Oligosaccharide based nutritional supplements
• T4 rII system
• 2011 E. coli O104:H4 outbreak

References[link]

External links[link]

Wikispecies has information related to: Escherichia coli

Wikimedia Commons has media related to: Escherichia coli

• Photo Blog post of Escherichia Coli
• E. coli Vs Organic Farming
• E. coli: Protecting yourself and your family from a sometimes deadly bacterium
• E. coli statistics
• Spinach and E. coli Outbreak – U.S. FDA
• E. coli Outbreak From Fresh Spinach – U.S. CDC
• Current research on Escherichia coli at the Norwich Research Park
• E. coli gas production from glucose video demonstration
• The correct way to write E. coli

Databases[link]

• EcoSal Continually updated Web resource based on the classic ASM Press publication Escherichia coli and Salmonella: Cellular and Molecular Biology
• Uropathogenic Escherichia coli (UPEC)
• ECODAB The structure of the O-antigens that form the basis of the serological classification of E. coli
• 2DBase 2D-PAGE Database of Escherichia coli University of Bielefeld – Fermentation Engineering Group (AGFT)
• 5S rRNA Database Information on nucleotide sequences of 5S rRNAs and their genes
• ACLAME A CLAssification of Mobile genetic Elements
• AlignACE Matrices that search for additional binding sites in the E. coli genomic sequence
• ArrayExpress Database of functional genomics experiments
• ASAP Comprehensive genome information for several enteric bacteria with community annotation
• Bacteriome E. coli DNA-Binding Site Matrices Applied to the Complete E. coli K-12 Genome
• BioGPS Gene portal hub
• BRENDA Comprehensive Enzyme Information System
• BSGI Bacterial Structural Genomics Initiative
• CATH Protein Structure Classification
• CBS Genome Atlas
• CDD Conserved Domain Database
• CIBEX Center for Information Biology Gene Expression Database
• COGs
• Coli Genetic Stock Center Strains and genetic information on E. coli K-12
• coliBASE
• EcoCyc – literature-based curation of the entire genome, and of transcriptional regulation, transporters, and metabolic pathways
• PortEco (formerly EcoliHub) – NIH-funded comprehensive data resource for E. coli K-12 and its phage, plasmids, and mobile genetic elements
• EcoliWiki is the community annotation component of PortEco