STS-100 was a Space Shuttle mission to the International Space Station (ISS) flown by Space Shuttle Endeavour. STS-100 installed the ISS Canadarm2 robotic arm.

The highest priority objectives of the flight were the installation, activation and checkout of the Canadarm2 robotic arm on the station. The operation of the arm is critical to the capability to continue assembly of the International Space Station, and was also necessary to attach a new airlock to the station on the subsequent shuttle flight, mission STS-104. A final component of the Canadarm is the Mobile Base System (MBS), installed onboard the station during the STS-111 flight.

Other major objectives for Endeavour’s mission were to berth the Raffaello logistics module to the station, activate it, transfer cargo between Raffaello and the station, and reberth Raffaello in the shuttle's payload bay. Raffaello is the second of three Italian Space Agency-developed Multi-Purpose Logistics Module that were launched to the station. The Leonardo module was launched and returned on the previous shuttle flight, STS-102, in March.