Summary
David Holden graduated from the University of Durham UK in 1977 and completed his PhD in Microbiology at University College London in 1981. He held postdoctoral fellowships in Canada and the USA before returning to the UK in 1988 to work in the Genetics Division at the National Institute for Medical Research, London. In 1990 he was appointed as a Lecturer at the Royal Postgraduate Medical School (RPMS), London. In 1995 he became full Professor of Molecular Microbiology. Holden was Director of the Centre for Molecular Bacteriology and Infection (MRC CMBI), between 2012 and 2019. In 2016, he was appointed as the first Regius Professor of Infectious Disease in the UK, conferred to Imperial College as part of the Queen's 90th birthday celebrations.
Holden is best known for inventing a technique called signature-tagged mutagenesis (also termed barcoding) for identification of mutants with altered growth in mixed populations. Using STM his group has identified numerous genes of Streptococcus, Staphylococcus and Salmonella that are required for virulence. The group currently comprises 12 researchers who study bacterial virulence mechanisms, in particular those of Salmonella. He is a Fellow of the Royal Society, the American Academy of Microbiology, the Academy of Medical Sciences (UK), and an EMBO Member. He co-founded the vaccine company Microscience, which was acquired in 2005 by Emergent Biosolutions. He has served on Board of Reviewing Editors for Science magazine and various scientific advisory boards.
Commercial Activity
Commercial spinoffs
founder, Microscience.
Publications
Journals
Cerny O, Godlee C, Tocci R, et al., 2021, CD97 stabilises the immunological synapse between dendritic cells and T cells and is targeted for degradation by the Salmonella effector SteD, Plos Pathogens, Vol:17, ISSN:1553-7366, Pages:1-28
Heggie A, Cerny O, Holden DW, 2021, SteC and the intracellular Salmonella-induced F-actin meshwork, Cellular Microbiology, Vol:23, ISSN:1462-5814
Alix E, Godlee C, Cerny O, et al., 2020, The tumor suppressor TMEM127 is a Nedd4-family E3 ligase adaptor required by Salmonella SteD to ubiquitinate and degrade MHC class II molecules, Cell Host and Microbe, Vol:28, ISSN:1931-3128, Pages:54-68.e7
Cerny O, Holden D, 2019, Salmonella SPI-2 type III secretion system-dependent inhibition of antigen presentation and T cell function, Immunology Letters, Vol:215, ISSN:0165-2478, Pages:35-39
Godlee C, Cerny O, durkin C, et al., 2019, SrcA is a chaperone for the Salmonella SPI-2 type three secretion system effector SteD, Microbiology, Vol:165, ISSN:1350-0872, Pages:15-25