HIS3

The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis. It is analogous to hisB in Escherichia coli.

Exploits

Mutations in Escherichia coli's analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of E. coli in which a desired protein-DNA or protein-protein interaction is taking place (Joung et al., 2000)

References

Calling (Lose My Mind)

"Calling (Lose My Mind)" is a single by Swedish house producers Sebastian Ingrosso (of Swedish House Mafia) and Alesso featuring American recording artist Ryan Tedder of the band OneRepublic. It was released in Sweden on March 13, 2012 and in the UK on May 27, 2012. Canadian recording artist Matthew Koma assisted the artists in writing the song. The original instrumental version of the track was released on August 31, 2011, under the name "Calling". The song topped the Billboard Hot Dance Club Songs chart and was also a top-ten hit in Australia and the UK. It was featured on Swedish House Mafia's second compilation album Until Now, released on October 22, 2012.

Chart performance

Charts

Year-end charts

Certifications

External links

References

Calling (Taproot song)

"Calling" is the first single released from Taproot's third studio album Blue-Sky Research. Like most of the songs on the album, it is significantly less heavy than their previous singles, and shows more of a standard alternative rock/post-grunge approach.

Music video

The song's music video was directed by Moh Azima.

Charts

Personnel

References

Smallville (season 2)

Season two of Smallville, an American television series developed by Alfred Gough and Miles Millar, began airing on September 24, 2002, on The WB television network. The series recounts the early adventures of Kryptonian Clark Kent as he adjusts to life in the fictional town of Smallville, Kansas, during the years before he becomes Superman. The second season comprises 23 episodes and concluded its initial airing on May 20, 2003. Regular cast members during season two include Tom Welling, Kristin Kreuk, Michael Rosenbaum, Sam Jones III, Allison Mack, Annette O'Toole and John Schneider. John Glover who was a recurring guest in season one was promoted to regular for season two. At the end of season one, Eric Johnson, who portrayed Whitney Fordman, had left the show.

Season two picks up directly where season one ended, with Clark (Welling) dealing with the aftermath of the tornadoes that hit Smallville. This season, Clark finally learns who he is and where he comes from, but must also acknowledge a potential destiny set into motion by his biological father that could change his life and the lives of those around him forever. Clark's relationship with Lana Lang (Kreuk) becomes increasingly closer, straining his friendship with Chloe Sullivan (Mack). Clark's best friend, Pete Ross (Jones III), learns Clark's secret this season.

First (David Gates album)

First is the first solo album by David Gates of Bread. The musicians include: Jimmy Getzoff, Jim Gordon, Jim Horn, John Guerin, Larry Carlton, Larry Knechtel, Louie Shelton, Mike Botts and Russ Kunkel. Suite, Clouds & Rain is definitely worth a listen as Gates experimented with his sound.

Track listing

All tracks composed by David Gates

First (Kandersteg)

The First is a mountain of the Bernese Alps, overlooking Kandersteg in the canton of Bern.

It summit can be reached by trail from Adelboden or Kandersteg.

References

External links

1st & Ten

1st & Ten, 1st and Ten, or First & Ten may refer to: