Some interesting stuff I noticed:

1. In the English version baby Arlecchino asks "do mothers usually argue with their daughters" but in CN she specifies that "do *birth/real* mothers fight their *birth/real* daughters?". Also if we look at the eye/hair colours of Clervie and the old Knave, we can make a connection that Clervie is the real birth daughter of their "mother" which makes the whole "I should have pruned this flower long ago" allegory that much more sickening.

2. In of the scene after Clervie dies and before Arlecchino goes to fight the old Knave, we see a dull blade with a bulle fruit ornament hanging from it. That's the same ornament the blond twin tailed girl had, so she's probably also dead. If we look back to the picture book in the beginning it shows a bunny with a bulle fruit hair ornament standing victoriously in front of a kneeling pink bunny (Clervie). I would argue that Clervie likely was killed by the blond girl who then perished at the hands of Arlecchino.

3. Speaking of the picture book, there's a Frankenstein's monster looking bat doctor shown in the scene. That's probably Doctore performing sick experiments on the orphans. Which is reinforced with the scene showing a syringe in the orphanage bedroom floor.