- published: 12 Nov 2009
- views: 699771
Dual expression recombinase based (DERB) single vector system is a method of efficient cloning and subcloning of plasmid vectors for high throughput screening (HTS) and verification of protein-protein interactions inside living cells. DERB was developed by Lu JP et al.
Plasmid Vectors are deliberately constructed circular double-strand DNA loops capable of self-amplification and protein production. They are widely used in laboratories and the bio-medical and pharmaceutical industries to produce of DNA or protein in quantity. The DERB vector system consists of a series of vectors, each of which produces two or more proteins which are labeled or tagged for screening and verification of molecular interactions such as protein–protein interactions.
The following characteristics of the vectors ensure highly efficient cloning or subcloning of the protein of interest ORFs into the DERB vectors for high-throughput screening (HTS) and verification of protein–protein interactions after single introducing of the vectors into eukaryotic or prokaryotic cells:
Scot Project (born Frank Zenker, 29 May 1973, Frankfurt, Germany) is a German hard trance DJ and producer. He also produces musical pieces under aliases such as "Arome" and "TOCS."
Scot Project learned to mix techno at the age of 13, and he got his first break in 1986, playing break beat in a youth club in Frankfurt. During the 1990s, he became resident DJ of several clubs in and around Frankfurt.
Scot Project's success began in 1994, when his first track "X" was released; this was followed closely by "U I Got A Feeling," in 1995. The latter track peaked at #66 in the UK Singles Chart. It was followed by the 1998 UK hit, "Y (How Deep is Your Love)" which made #57.
In 2002, Frank Zenker and Kai Winter (Derb) created the Druck Records label.
In 2004, Scot's track "L (Want Your Love)" took over from Eric Prydz's "Call On Me" at #1 in the charts.
DERB - DERB please rate it and write comments =) and to subscribe my channel ;-)
OUT NOW: http://smarturl.it/DERPIT Hysteria Facebook: http://www.facebook.com/hysteriarecords Hysteria Twitter: http://twitter.com/hysteriarecords Hysteria Instagram: https://instagram.com/hysteriarecords Hysteria Soundcloud: https://soundcloud.com/hysteriarecords Twitter Bassjackers: https://twitter.com/bassjackers Facebook Bassjackers: https://facebook.com/Bassjackers Soundcloud Bassjackers: https://soundcloud.com/bassjackers Twitter MAKJ: https://twitter.com/DJMAKJ Facebook MAKJ: https://www.facebook.com/MAKJOfficial Soundcloud MAKJ: https://soundcloud.com/makj Camera & Editing : Shane Muller Www.shanemuller.nl BASSJACKERS AND MAKJ SMASH HIT DERP
wenn ihr ein anderes Lied gern Hören wollt schreibt mich an mit @HARDSTYLE und ich mach mein bestes draus ;) ~Hardstyle is my style~
Over? Did you say it's over? Nothing is over until we decide it is!!! Early Hardstyle Never Die!!! ___________________ COPYRIGHT NOTICE: If YOU are the owner of the track or the official label, please send me a private message via youtube to delete the track except of contacting youtube!! I will accept your decision and i will delete the track as soon as possible I would really appreciate that :) Tracklist 01. ID (ID - ID) 02. Derb - Coole Sau (Isaac Remix) 03. Thomas Rubin - Cold Night (DJ Scot Project Remix) 04. Hennes & Cold - Second Trip (DJ Scot Project Remix) 05. Arome - Hands Up! (DJ Scot Project Remix) 06. A.S.Y.S. - Acid Nightmare 07. Derb - In Africa 08. Kernkraft - Zombie Nation 09. Spacefrog - Follow Me (Derb Remix) 10. Elektrochemie LK - Schall 11. D...
One of my favorite songs ever hope you enjoy as much as me!
Get it here: https://grotesque.choons.at/derbus Remember this stunner from back in the days? Well, Grotesque label boss RAM himself decided to pimp it up a whole lot… He enriches the original with a powerful and blazing synth line, finishing it off with a massive drop, making it the perfect anthem for the upcoming festival season! It’s fast-paced and infectious, aiming to destroy every dance floor making you jump till exhaustion. Subscribe to Black Hole Recordings TV: https://blackhole.choons.at/youtube Spotify: https://blackhole.choons.at/spotify Facebook: https://www.facebook.com/blackholerecordings Twitter: http://twitter.com/BlackHoleRec
Daily uploads - to delivering you the Best and Freshest Bounce ► Mikey P - Derb • Download - https://soundcloud.com/mikeypdj/derb ---------------------------------------------------------------------------- • Support Mikey P » https://soundcloud.com/mikeypdj » https://www.facebook.com/mikeypofficial » http://www.twitter.com/mikeypdj » http://www.youtube.com/djmikeypuerari » https://instagram.com/mikeypdj ---------------------------------------------------------------------------- • Stay Connected » Subscribe For Daily EDM ? [http://bit.do/TheBounceUnited] » Facebook: https://www.facebook.com/Bounceunited ---------------------------------------------------------------------------- • Picture - http://wall.alphacoders.com/big.php?i=463768 This channel is strictly based on the prom...
I'm trying to walk up the stairs
My hands are snatchin' at the slivers of light
I'm stickin' to the steps
Each one a release
From the place below
I'm on a mission
On the hunt for clean
Clear vapour skies
'Cos I'm choking on my own
I need some air
The dark skies behind me
Begging you to scrape off, your disco paint
It's open to the night
And I'm sick as a hospital
And empty factories
You look so tiny, so very unimportant
I'm nearly there, and everything feels fine
Don't wanna look behind me
Don't wanna look beneath me
Every movement
Every vibration
Every movement
Every vibration
High above piss city
Watch the pigs-ear people
All dead in their drows
Some suffer in silence
Someone sparks
Some gorge sucking on silicone
I've got the urge to jump
Watch life whizz by
Fast foward flash
But hold on boy
I feel... alive
Don't wanna look behind me
Don't wanna look beneath me
Every movement
Every vibration
Every movement
Every vibration
Don't wanna look behind me
Don't wanna look beneath me
Every movement
Every vibration
Every movement
Every vibration
Don't wanna look behind me
Don't wanna look beneath me
Every movement
Every vibration
Every movement