The Kohathites were one of the three main divisions among the Levites in Biblical times, the other two being the Gershonites and the Merarites. The Bible claims that the Kohathites were all descended from the eponymous Kohath, a son of Levi, although some biblical scholars regard this as a postdictional metaphor providing an aetiology of the connectedness of the clan to others in the Israelite confederation; according to some Jewish scholars, Levite was originally just a job title, deriving from the Minaean word lawi'u meaning priest, rather than having been the name of a tribe. Although the Aaronids are described as part of the Kohathites, the text strongly differentiates between the Aaronids and the other Kohathites.
The Bible ascribes a specific religious function to the Kohathites, namely care of the vessels and objects within the sanctuary - the Ark of the Covenant, Menorah, Table of Shewbread, etc. This differentiation of religious activity between the Kohathites and other Levites, even the Aaronids, is found only in the Priestly Code, and not in passages that textual scholars attribute to other authors.