Yo-yo

A yo-yo (also spelled yoyo) is a toy which in its simplest form is an object consisting of an axle connected to two disks, and a length of string looped around the axle, similar to a slender spool. It is played by holding the free end of the string known as the handle (usually by inserting one finger in a slip knot) allowing gravity or the force of a throw to spin the yo-yo and unwind the string (similar to how a pullstring works), then allowing the yo-yo to wind itself back to one's hand, exploiting its spin (and the associated rotational energy). This is often called "yo-yoing". First made popular in the 1920s, yo-yoing remains a popular pastime of many generations and cultures. It was first invented in ancient Greece.

In the simplest play, the string is intended to be wound on the spool by hand; The yo-yo is thrown downwards, hits the end of the string, then winds up the string toward the hand, and finally the yo-yo is grabbed, ready to be thrown again. One of the most basic tricks is called the sleeper, where the yo-yo spins at the end of the string for a noticeable amount of time before returning to the hand.

YOYO-1

YOYO-1 is a green fluorescent dye used in DNA staining. It belongs to the family of monomethine cyanine dyes and is a tetracationic homodimer of Oxazole Yellow (abbreviated YO, hence the name YOYO), typically available as tetraiodide salt. In aqueous buffer, free YOYO-1 dye (absorption: λ_max 458 nm, emission: λ_max 564 nm) has very low fluorescence quantum yield, however the intensity of fluorescence increases 3200 times upon binding to double-stranded DNA (absorption: λ_max 489 nm, emission: λ_max 509 nm).

Synthesis

YOYO-1 is prepared by alkylation of N,N,N’,N’-tetramethyl-1,3-propanediamine with 2 equivalents of N-(3-iodopropyl) analogue of Oxazole Yellow, which is available in three steps from 2-mercaptobenzoxazole:

References

Blood

Blood is a bodily fluid in humans and other animals that delivers necessary substances such as nutrients and oxygen to the cells and transports metabolic waste products away from those same cells. When it reaches the lungs, gas exchange occurs when carbon dioxide is diffused out of the blood into the pulmonary alveoli and oxygen is diffused into the blood. This oxygenated blood is pumped to the left hand side of the heart in the pulmonary vein and enters the left atrium. From here it passes through the mitral valve, through the ventricle and taken all around the body by the aorta. Blood contains antibodies, nutrients, oxygen and much more to help the body work.

In vertebrates, it is composed of blood cells suspended in blood plasma. Plasma, which constitutes 55% of blood fluid, is mostly water (92% by volume), and contains dissipated proteins, glucose, mineral ions, hormones, carbon dioxide (plasma being the main medium for excretory product transportation), and blood cells themselves. Albumin is the main protein in plasma, and it functions to regulate the colloidal osmotic pressure of blood. The blood cells are mainly red blood cells (also called RBCs or erythrocytes), white blood cells (also called WBCs or leukocytes) and platelets. The most abundant cells in vertebrate blood are red blood cells. These contain hemoglobin, an iron-containing protein, which facilitates oxygen transport by reversibly binding to this respiratory gas and greatly increasing its solubility in blood. In contrast, carbon dioxide is almost entirely transported extracellularly dissolved in plasma as bicarbonate ion.

Vs. (Pearl Jam album)

Vs. is the second studio album by the American rock band Pearl Jam, released on October 19, 1993 through Epic Records. After a relentless touring schedule in support of their 1991 debut album Ten, Pearl Jam headed into the studio in early 1993 facing the challenge of following up the commercial success of its debut. The resulting album, Vs., featured a rawer and more aggressive sound compared with the band's previous release. It was the band's first collaboration with producer Brendan O'Brien.

Pearl Jam decided to scale back its commercial efforts for Vs., including declining to produce music videos for any of the album’s singles. Upon its release, Vs. set the record for most copies of an album sold in its first week, a record it held for five years. Vs. occupied the number one spot on the Billboard 200 chart for five weeks, the longest duration for a Pearl Jam album. The album has been certified seven times platinum by the RIAA in the United States.

Recording

For its second album, Pearl Jam felt the pressures of trying to match the success of its debut album, Ten. In a 2002 interview, guitarist Mike McCready said, "The band was blown up pretty big and everything was pretty crazy."Vs. was the first Pearl Jam album to have production duties handled by producer Brendan O'Brien. It was also the band's first album with drummer Dave Abbruzzese, who had joined the band in August 1991 and toured for the album Ten. Rehearsals for Vs. began in February 1993 at Potatohead Studio in Seattle, Washington. The band then moved to The Site in Nicasio, California in March 1993 to begin recording. Abbruzzese called the tranquil recording site "paradise" while lead vocalist Eddie Vedder said, "I fucking hate it here...I've had a hard time...How do you make a rock record here?"

Blood (TV series)

Blood (Hangul: 블러드; RR: Beulleodeu) is a 2015 South Korean television series starring Ahn Jae-hyun, Ji Jin-hee, Ku Hye-sun and Son Soo-hyun. It aired on KBS2 from February 16 to April 21, 2015 on Mondays and Tuesdays at 22:00 for 20 episodes.

Plot

Park Ji-sang is a doctor specializing in hepato-pancreato-biliary surgery at Taemin Cancer Hospital, the best hospital in the country. He is also a vampire. Despite seeming cold and unfeeling, Ji-sang masks his soft heart and inner pain and yearns for closeness with people. He believes very strongly in the sanctity of human life, and suppresses his thirst for blood to treat terminally ill patients and save lives.

Among his colleagues is Yoo Ri-ta, a hotshot physician who entered medical school at the age of 17 and is also the niece of the chaebol group chairman who owns the hospital. Ri-ta is highly capable but snooty and prideful, yet Ji-sang finds himself falling for her. He also gets drawn into a conflict between good and evil as he encounters Lee Jae-wook, a two-faced hospital chief who gains everyone's trust with his gentle demeanor, but inwardly harbors a dangerous ambition for power and a talent for cruelty.