Hi-Risers are a type of highly customized automobile, typically a traditional, full-size, body on frame, V8 powered, rear wheel drive American-built sedan modified by significantly increasing the ground clearance and adding large-diameter wheels with low-profile tires. Depending on the model and style of body, autos customized in this manner can be labeled "donk," "box," or "bubble."
Hi-risers originally grew out of the Dirty South subculture, but the trend has spread across the United States. Vehicles customized in the hi-riser style are distinguished by their oversized (even disproportionate) wheels, ranging from 20 inches to 30 inches or more in diameter (largest being 50 inch), as well as fanciful custom paint-jobs and expensive audio equipment. Suspension modifications similar to those employed on lifted pickup trucks are made to give adequate clearance for the large wheels. Often the suspension is modified so the front end sits slightly higher than the rear end, giving the car a swaggering appearance. Because of the exaggerated look gained from installing a lifted suspension and enormous wheels, donks are also known as "hi-risers" or "sky-scrapers."
"Donk" is the fifth and final single by American rapper Soulja Boy's first studio album, souljaboytellem.com.
A remix to the song was released in mid 2008. The remix features Yung Joc.
This song was sampled on the track "Pop It 4 Pimp" with Juvenile and Webbie on Bun B's 2nd album II Trill. The "Yup!" and the "Chase on wit' it!" lyrics are what that song samples. It was also sampled on Nicki Minaj's "Itty Bitty Piggy" on her third mixtape, Beam Me Up Scotty.
Bouncy techno is a hardcore dance music rave style that developed in the early 1990s from Scotland and North England. Described as an accessible gabber-like form, it was popularised by Scott Brown under numerous aliases. The sound became prominent in the northern United Kingdom rave scene before it broke into the hardcore homeland of the Netherlands through Paul Elstak, where it became known there as happy hardcore (i.e.: happy gabber) and funcore .
A subsequent mainstream-aimed Eurodance tangent appeared in Germany and itself back into the Netherlands. The music of Brown also changed the Southern England happy breakbeat style away from its breakbeat foundation and into a bouncy derivative. These different country entrails created a single pan European hardcore briefly in the mid-1990s. Bouncy techno rapidly declined from this point for a variety of reasons.
The breakbeat hardcore style that dominated raves across England was generally not popular in Scotland. This is attributed to regional music,cultural and racial differences across the UK; with breakbeat regarded in Scotland as a "black English thing" and an "alien musical culture".
Deoxyribonucleic acid (i/diˈɒksiˌraɪboʊnjʊˌkliːɪk, -ˌkleɪɪk/;DNA) is a molecule that carries most of the genetic instructions used in the development, functioning and reproduction of all known living organisms and many viruses. DNA is a nucleic acid; alongside proteins and carbohydrates, nucleic acids compose the three major macromolecules essential for all known forms of life. Most DNA molecules consist of two biopolymer strands coiled around each other to form a double helix. The two DNA strands are known as polynucleotides since they are composed of simpler units called nucleotides. Each nucleotide is composed of a nitrogen-containing nucleobase—either cytosine (C), guanine (G), adenine (A), or thymine (T)—as well as a monosaccharide sugar called deoxyribose and a phosphate group. The nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone. According to base pairing rules (A with T, and C with G), hydrogen bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA. The total amount of related DNA base pairs on Earth is estimated at 5.0 x 1037, and weighs 50 billion tonnes. In comparison, the total mass of the biosphere has been estimated to be as much as 4 TtC (trillion tons of carbon).
DNA2.0 provides products and services for life science and Synthetic biology research. DNA2.0 also provides free access to research tools such as Gene Designer, DNA Atlas and a gRNA designer.
DNA2.0 was founded in 2003, in Menlo Park, California. The company is privately held and continues to have all research, development and production in Menlo Park, California. It began and continues as a gene synthesis and protein engineering provider to academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. Gene Synthesis rapidly replaced molecular cloning for many academic and corporate labs, as "foundries for the biotechnology age" allowing made-to-order genes for biological research. DNA2.0 was featured on the PBS show Nova ScienceNow to show how genes are created synthetically in a lab. In 2008, the company supplied some of the DNA stretches used to create a synthetic bacterial genome.Dan Rather Reports included DNA2.0 in their episode on Synthetic Biology and how it is solving "some of the most important problems facing the world." In 2009, The Scientist named the codon design algorithms (now tradmarked as GeneGPS) developed by DNA2.0 as one of the Top 10 Innovations of the year for Life Sciences. DNA2.0 developed the Electra Vector System, a universal cloning system that utilizes the type IIS restriction enzyme SapI and T4 DNA ligase in a single-tube reaction. DNA2.0 has made some molecular components, such as synthetic fluorescent proteins, available in open-access collections of DNA parts (BioBricks Foundation). DNA2.0 is a founding member of the International Gene Synthesis Consortium (IGSC) to promote biosecurity in the gene-synthesis industry. There are over 1,100 published scientific articles using DNA2.0 products and/or services, of which 43 include company employees as an author(s).