A V/Line train carrying about 80 passengers has been derailed after hitting a car abandoned on the tracks near Bendigo.
Passengers were forced to exit the train in scrub near Kangaroo Flat.
The car was parked across the tracks.
V/Line is arranging replacement buses between Bendigo and Melbourne.
Paramedics are at the scene, but there are no confirmed reports of serious injuries at this stage.
V/Line said two passengers have minor injuries.
"V/Line can confirm there has been an incident involving a car and a V/Line train near Phillis Street, Kangaroo Flat, on the Bendigo line," V/Line said.
"Emergency services are at the site which is approximately 140 kilometres north of Melbourne."
The train involved is the 12.14pm Southern Cross Station to Epsom service, with approximately 80 passengers, a conductor and driver on board.
All services on the Bendigo line have been suspended until further notice.
The collision happened on the same day V/Line released startling vision of a near-hit on the Ballarat line.
A group of trail bike riders were riding on the railway tracks, one of whom came within centimetres of being hit and killed by a high-speed train.
Train service alterations will be in place this afternoon on the Bendigo line due to a train striking an abandoned car near Kangaroo Flat.
— V/Line Bendigo Line (@vline_bendigo) February 15, 2017
A @vline_bendigo train has struck an abandoned car near #KangarooFlat. Eight people being treated by @AmbulanceVic. https://t.co/YzcafkXmtA pic.twitter.com/ZT3W728zu3
— VicEmergency (@vicemergency) February 15, 2017
A V/Line train has derailed at Kangaroo Flat pic.twitter.com/hwLN4WJg0X
— Natalie Croxon (@natcroxon) February 15, 2017