The ACT's special outlaw motorcycle gang police taskforce is investigating a suspicious fire at the Comancheros Fyshwick clubhouse early Thursday morning.
At about 12.15am police arrived to the Isa Street building to find it well alight, with ACT Fire and Rescue battling the fire.
Firefighters extinguished the fire, which ACT Policing's Detective Superintendent Ben Cartwright said left the building significantly damaged.
"The exact cost of that damage is yet to be determined," he said.
"Inside there was extensive damage to property and there was smoke and water damage to either side of the premises."
Superintendent Cartwright said he was not aware of anyone being in or near the building at the time of the fire.
Police established a crime scene overnight which remained in place on Thursday morning.
At 8am, two ACT Fire & Rescue vehicles and one police van were parked on the driveway between the Auto Drive Transmissions store and Eglaze Australia.
An Isa Street business owner said he regularly saw bikies "hanging around the shed" down the driveway between the two businesses.
A civil dispute between the owner of the building and the Comancheros, one of Canberra's three bikie gangs, will form part of police enquiries.
Superintendent Cartwright said it was too early to determine whether other bikie gangs were involved and police would look at all possible motivations as well as how the fire was lit.
Superintendent Cartwright expressed confidence in the ACT Policing's anti-bikie arm, which he said was doing "everything in its powers" to target bikie-related crime.
"These people who commit these crimes, be it gang members or any other members of the community, must understand ACT policing take it very seriously," he said.
"They could endanger anyone. We will do anything we can to put them before the courts."
Before 2014, the Rebels was the only known bikie gang in the ACT.
Since joined by Comancheros and Nomads, tensions between the rival gangs have erupted, with shootings at Canberra homes and a a targeted arson attack at a Tuggeranong tattoo parlour.
The federal government recently announced a national anti-gangs squad officer will join ACT Policing from February 1, giving the squad a presence in every Australian state and territory for the first time.
It's expected the National Anti-Gangs Squad liaison officer will bolster the work of Taskforce Nemesis, by sharing information about bikie activities from the other states and territory.
Last year the ACT government ditched its proposal to establish anti-consorting laws, used in NSW and Queensland to disrupt bikie and crime groups by preventing their members from meeting or speaking.
The government cited struggles to draft laws that satisfy police while respecting human rights.
The proposal was opposed by Greens minister Shane Rattenbury, much of Labor's Left faction and the Human Rights Commission.
On Friday, Superintendent Cartwright reiterated ACT Policing had a clear view on anti-consorting but "it was a matter for the ACT government".
Police will assess CCTV footage from businesses on Isa Street but urged anyone with information regarding Thursday's fire to contact Crime Stoppers on 1800 333 000 and quote reference number 6060766.
with Finbar O'Mallon