White spot disease has been found to be infecting prawns in another farm on the Logan river on Friday.
A fifth prawn farm has tested positive for the highly contagious virus more than a month after the initial case was identified.
The farm has been destocked and chlorine was being used to treat the water at the premises four kilometres down river from the first case.
Biosecurity Queensland has ramped up surveillance at nearby farms and locations in an effort to keep the virus in check.
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The government department is also investigating the source of the virus, testing a 'range of materials' to identify potential for further spread.
Since the first case the movement of all crustaceans, worms and salt water has been halted in the Albert and Logan rivers which are under a biosecurity emergency order.
Testing will continue on the Logan, Brisbane and Pine rivers as well as in Moreton Bay, but Biosecurity Queensland does not believe the disease has established itself in the wild crustacean population.
The aquaculture industry is currently developing a compensation scheme, but has not been finalised and farmers will not have access to compensation other than statutory compensation.
Statutory compensation is only available for stock that would not have become infected and those provisions have not been enacted.