- published: 07 Dec 2014
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The patch clamp technique is a laboratory technique in electrophysiology that allows the study of single or multiple ion channels in cells. The technique can be applied to a wide variety of cells, but is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells. It can also be applied to the study of bacterial ion channels in specially prepared giant spheroplasts.
The patch clamp technique is a refinement of the voltage clamp. Erwin Neher and Bert Sakmann developed the patch clamp in the late 1970s and early 1980s. This discovery made it possible to record the currents of single ion channel molecules for the first time, which improved understanding of the involvement of channels in fundamental cell processes such as action potentials and nerve activity. Neher and Sakmann received the Nobel Prize in Physiology or Medicine in 1991 for this work.
Patch clamp recording uses a glass micropipette called a patch pipette as a recording electrode, and another electrode in the bath around the cell, as a reference ground electrode. Depending on what the researcher is trying to measure, the diameter of the pipette tip used may vary, but it is usually in the micrometer range. This small size is used to enclose a membrane surface area or "patch" that often contains just one or a few ion channel molecules. This type of electrode is distinct from the "sharp microelectrode" used to puncture cells in traditional intracellular recordings, in that it is sealed onto the surface of the cell membrane, rather than inserted through it.
From Purves neuroscience fifth edition. © 2012 Sinauer Associates, Inc.
This lecture explains about the patch clamp technique process and the set up and recording with patch clamp technique. It also explains the role of patch clamp technique to understand the action potential and membrane potential of a neuron. The patch clamp technique is a laboratory technique in electrophysiology that allows the study of single or multiple ion channels in cells. The technique can be applied to a wide variety of cells, but is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells. It can also be applied to the study of bacterial ion channels in specially prepared giant spheroplasts. The patch clamp technique is a refinement of the voltage clamp. Erwin Neher and Bert Sakmann developed the patch clamp in the...
We discuss the Patch clamp technique and how it contributes to our understanding of voltage gated ion channels.
Virtual tour of whole cell patch clamp electrophysiology at the Allen Institute for Brain Science.
In this first part of the unscripted video, I give you a very simplistic overview of what equipment you might need or encounter when setting up a single-cell patch clamp rig for whole-cell patch and electrophysiological recordings from neurons. Video # 2 here: http://youtu.be/Kjfny-0nvJM In video: 0:00-2-26: Intro and assumptions 2:27-11:22: General overview of what a patch-clamp is 11:23-16:45: Microscope, air table, fixed stage, microscope manipulator 16:45-17:45: Cameras for imaging and recording NOTE: I do not endorse vendors, and my opinions are based on what works for me. I suggest using this as a start-point to look up equipment that will be relevant for your own applications. Thanks!
In this second part of the unscripted video, I give you a very simplistic overview of what equipment you might need or encounter when setting up a single-cell patch clamp rig for whole-cell patch and electrophysiological recordings from neurons. Video # 3 here: http://youtu.be/KadJsXFMsPA In video: 0:00-3:41: Recording chamber, perfusion methods, in-line heater 3:42-7:18: Headstage, micromanipulator 7:25-10:03: Use of Faraday cage, grounding wire 10:04-11:23: Where to physically place the set-up in a lab
Researchers at Georgia Tech and MIT have developed a way to automate the process of finding and recording information from neurons in the living brain. The researchers have shown that a robotic arm guided by a cell-detecting computer algorithm can identify and record from neurons in the living mouse brain with better accuracy and speed than a human experimenter. Using this technique, scientists could classify the thousands of different types of cells in the brain, map how they connect to each other, and figure out how diseased cells differ from normal cells. Reference: S. Kodandaramaiah, G. Franzesi, B. Chow, E. Boyden, C.R. Forest, Automated whole-cell patch clamp electrophysiology of neurons in vivo. Nature Methods, Vol. 9(6), p. 585-587, May 2012. (www.nature.com/nmeth/journal/v9/n6/a...
Con este vídeo se pretende ayudar al estudiante de los grados y postgrados de materias relacionadas con Ciencias de la Salud a: Conocer los materiales y procedimientos para el registro intracelular de la actividad eléctrica de las neuronas, mediante preparaciones in vitro y utilizando la técnica de “patch clamp” Conocer los protocolos que permiten definir la excitabilidad neuronal y los tipos de neurotransmisores / receptores postsinápticos implicados en la transmisión de información entre neuronas. ISBN: 978-84-16326-03-7
Odpovědi nervových buněk na přirozené neuropřenašeče i látky jako nikotin studujeme pomocí metody terčíkového zámku (patch clamp). Více o této metodě i oblastech výzkumu, ve kterých ji využíváme, se dočtete na stránkách Oddělení buněčné neurofyziologie (http://www.fgu.cas.cz/departments/bunecna-neurofyziologie).
From Purves neuroscience fifth edition. © 2012 Sinauer Associates, Inc.
This lecture explains about the patch clamp technique process and the set up and recording with patch clamp technique. It also explains the role of patch clamp technique to understand the action potential and membrane potential of a neuron. The patch clamp technique is a laboratory technique in electrophysiology that allows the study of single or multiple ion channels in cells. The technique can be applied to a wide variety of cells, but is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells. It can also be applied to the study of bacterial ion channels in specially prepared giant spheroplasts. The patch clamp technique is a refinement of the voltage clamp. Erwin Neher and Bert Sakmann developed the patch clamp in the...
We discuss the Patch clamp technique and how it contributes to our understanding of voltage gated ion channels.
Virtual tour of whole cell patch clamp electrophysiology at the Allen Institute for Brain Science.
In this first part of the unscripted video, I give you a very simplistic overview of what equipment you might need or encounter when setting up a single-cell patch clamp rig for whole-cell patch and electrophysiological recordings from neurons. Video # 2 here: http://youtu.be/Kjfny-0nvJM In video: 0:00-2-26: Intro and assumptions 2:27-11:22: General overview of what a patch-clamp is 11:23-16:45: Microscope, air table, fixed stage, microscope manipulator 16:45-17:45: Cameras for imaging and recording NOTE: I do not endorse vendors, and my opinions are based on what works for me. I suggest using this as a start-point to look up equipment that will be relevant for your own applications. Thanks!
In this second part of the unscripted video, I give you a very simplistic overview of what equipment you might need or encounter when setting up a single-cell patch clamp rig for whole-cell patch and electrophysiological recordings from neurons. Video # 3 here: http://youtu.be/KadJsXFMsPA In video: 0:00-3:41: Recording chamber, perfusion methods, in-line heater 3:42-7:18: Headstage, micromanipulator 7:25-10:03: Use of Faraday cage, grounding wire 10:04-11:23: Where to physically place the set-up in a lab
Researchers at Georgia Tech and MIT have developed a way to automate the process of finding and recording information from neurons in the living brain. The researchers have shown that a robotic arm guided by a cell-detecting computer algorithm can identify and record from neurons in the living mouse brain with better accuracy and speed than a human experimenter. Using this technique, scientists could classify the thousands of different types of cells in the brain, map how they connect to each other, and figure out how diseased cells differ from normal cells. Reference: S. Kodandaramaiah, G. Franzesi, B. Chow, E. Boyden, C.R. Forest, Automated whole-cell patch clamp electrophysiology of neurons in vivo. Nature Methods, Vol. 9(6), p. 585-587, May 2012. (www.nature.com/nmeth/journal/v9/n6/a...
Con este vídeo se pretende ayudar al estudiante de los grados y postgrados de materias relacionadas con Ciencias de la Salud a: Conocer los materiales y procedimientos para el registro intracelular de la actividad eléctrica de las neuronas, mediante preparaciones in vitro y utilizando la técnica de “patch clamp” Conocer los protocolos que permiten definir la excitabilidad neuronal y los tipos de neurotransmisores / receptores postsinápticos implicados en la transmisión de información entre neuronas. ISBN: 978-84-16326-03-7
Odpovědi nervových buněk na přirozené neuropřenašeče i látky jako nikotin studujeme pomocí metody terčíkového zámku (patch clamp). Více o této metodě i oblastech výzkumu, ve kterých ji využíváme, se dočtete na stránkách Oddělení buněčné neurofyziologie (http://www.fgu.cas.cz/departments/bunecna-neurofyziologie).
This lecture explains about the patch clamp technique process and the set up and recording with patch clamp technique. It also explains the role of patch clamp technique to understand the action potential and membrane potential of a neuron. The patch clamp technique is a laboratory technique in electrophysiology that allows the study of single or multiple ion channels in cells. The technique can be applied to a wide variety of cells, but is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells. It can also be applied to the study of bacterial ion channels in specially prepared giant spheroplasts. The patch clamp technique is a refinement of the voltage clamp. Erwin Neher and Bert Sakmann developed the patch clamp in the...
Covers electrophysiology methods used to study ion channels and examples of ion channel diversity. 2:44 Voltage clamp methods 6:29 Patch clamp: an extension of voltage-clamp 9:18 Na+ current voltage dependence 11:13 K+ current voltage dependence 11:38 Types of current recorded 15:46 Rectifying K+ currents 16:42 K+ channel rectification in IV plots 19:11 IUPHAR/BPS Guide to Pharmacology 20:04 Ion channel families 21:00 Ion channel diversity 21:52 Distinct Ca2+ channel currents mediated by different alpha subunits 23:26 Distinct K+ currents mediated by related alpha subunits 24:21 Heteromeric vs. homomeric Kv channels 25:36 Why so many ion channel subtypes?
Selectivity & their mechanisms, patch clamp, question answering
Abstract The command signal such as voltage step or current injection is applied to the recorded cell during the whole-cell patch-clamp experiment. The generation of command waveforms in the protocol is critically important in the experimental design. In this webinar, Dr. Jeffrey Tang demonstrates how to employ useful features in Axon™ pCLAMP™ Software in order to create customized command waveforms for fulfilling the needs of each individual experimental design. Learn how to create customized command waveforms such as: - Customized analog output in sweep basis - Theta burst stimulation - Action potential waveform - Sine waveform - Chirp waveform - Paired-pulse stimulation with varied inter-pulse intervals
This tutorial was given as part of the HEKA Electrophysiology Update webinar on on June 27, 2013. The HEKA Electrophysiology Update is a series of webinars and user meetings that provide step-by-step guidance to the researcher who is new to the field and showcase new products to the experienced user. The webinar on June 27, 2013 webinar featured three presentations: - Telly Galiatsatos, BS, General Manager, HEKA Instruments USA: Chart Recording with PATCHMASTER™ Software - Jan Dolzer, PhD, Vice President Sales & Marketing, HEKA Elektronik: Where Two Worlds Meet: The EPC 800 Dual-control Patch Clamp Amplifier - Hubert Affolter, PhD, Senior Software Architect, HEKA Elektronik Germany: Combining Imaging and Electrophysiology A video showing the complete event, and PDF versio...
Presentation by Timm Danker from NMI, Germany: Automated patch clamp on cardiac ion channels and multiwell MEA recordings on Human iPSC-derived cardiomyocytes: a complementary approach for predictable proarhytmia assessments
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