- published: 14 Oct 2013
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Dolly (5 July 1996 – 14 February 2003) was a female domestic sheep, and the first mammal to be cloned from an adult somatic cell, using the process of nuclear transfer. She was cloned by Ian Wilmut, Keith Campbell and colleagues at the Roslin Institute and the biotechnology company PPL Therapeutics near Edinburgh in Scotland. She was born on 5 July 1996 and she lived until the age of six, at which point she died from a progressive lung disease. She has been called "the world's most famous sheep" by sources including BBC News and Scientific American. The cell used as the donor for the cloning of Dolly was taken from a mammary gland, and the production of a healthy clone therefore proved that a cell taken from a specific part of the body could recreate a whole individual. On Dolly's name, Wilmut stated "Dolly is derived from a mammary gland cell and we couldn't think of a more impressive pair of glands than Dolly Parton's".
Dolly was born on 5 July 1996 to three mothers (one provided the egg, another the DNA and a third carried the cloned embryo to term). She was created using the technique of somatic cell nuclear transfer, where the cell nucleus from an adult cell is transferred into an unfertilised oocyte (developing egg cell) that has had its nucleus removed. The hybrid cell is then stimulated to divide by an electric shock, and when it develops into a blastocyst it is implanted in a surrogate mother. Dolly was the first clone produced from a cell taken from an adult mammal. The production of Dolly showed that genes in the nucleus of such a mature differentiated somatic cell are still capable of reverting back to an embryonic totipotent state, creating a cell that can then go on to develop into any part of an animal. Dolly's existence was announced to the public on 22 February 1997..
The Sheep is a character, created by Charles Lutwidge Dodgson, aka Lewis Carroll. It appeared in Dodgson's book, Through the Looking-Glass, the sequel to his book Alice's Adventures in Wonderland.
The Sheep is first mentioned in the fifth chapter of Through the Looking-Glass, "Wool and Water". The White Queen is talking to Alice, when she suddenly starts "baa-ing" and then seems to 'wrap herself in wool'. Alice figures out she is in a shop, and that The White Queen has turned into a sheep. The Sheep sits in her chair knitting as Alice looks around the shop. She gives Alice a pair of her knitting needles, and asks her if she can row. As Alice begins to answer, she realizes that they are in a little boat, and that the needles have turned into oars. As they glide along the water, the Sheep repeatedly shouts out "Feather," and tells Alice that they will be catching crabs. Alice's attention is then put onto some scented rushes growing in the water. She tries picking them, but they are only 'dream rushes' and melt away. She then quickly catches a crab, which she actually didn't see, and they are all suddenly in the shop again. Alice buys an egg from the Sheep (that ends up turning into Humpty Dumpty) and the two part ways.