Trail Center is an unincorporated community in Monroe County, Florida, United States. It is located in the Big Cypress National Preserve, about 4 miles (6.4 km) northwest of Fortymile Bend.
It is located at 25°48′18″N 80°52′34″W / 25.805°N 80.876°W / 25.805; -80.876, with an elevation of 7 feet (2 m).
Coordinates: 25°48′18″N 80°52′34″W / 25.805°N 80.876°W / 25.805; -80.876