Human Fibroblast-Like Synoviocytes-Rheumatoid Arthritis: HFLS-RA

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• published: 20 Aug 2009
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Human Fibroblast-Like Synoviocytes isolated from an RA patient undergoing normal growth in culture (for 4 days post-thawing, with medium changed every other day). Note the elongated fibroblast-like shape of the cells and healthy cell divisions throughout the observation period. Fibroblast-like synoviocytes* are found in the joints, where they are responsible for producing glycoproteins that make up most of the synovial fluid, essential for joint lubrication. Like the name suggests, these cells are related to fibroblasts and share the origins and some of the markers with them. During rheumatoid arthritis, the joint becomes constantly inflamed, and, concurrently, synoviocytes acquire abnormal features and altered gene expression, contributing to destruction of the joint tissue. * See also: www.cellapplications.com/primary-cells/human-fibroblast-synoviocytes-rheumatoid-arthritis-hfls-ra At Cell Applications, Inc.primary Human Fibroblast-Like Synoviocytes-Rheumatoid Arthritis (HFLS-RA) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doublings. The cells can be shipped frozen or proliferating, with all the media reagents included. HFLS are long known for their role in joint destruction by producing cytokines and metalloproteinases that contribute to cartilage degradation.(1,2) Proinflammatory cytokines induce the proliferation, collagenase and aggrecanase production and GM-CSF secretion on HFLS.(3,4) Ongoing arthritis research also has shown that HFLS express apoptosis and P53 mutations.(5,6) Cell Applications, Inc. provides three types of HFLS that are useful cellular models for studying the differences between HFLS-RA and HFLS-OA, such as the expression and regulation of proteases(7) and integrin subunits.(8). * Normal HFLS (from healthy individuals) * HFLS-RA (from patients with Rheumatoid Arthritis) * HFLS-OA (from patients with Osteoarthritis) 1. Firestein, G.S. et al, J. Immunol. 149:1054 (1992)* 2. Firestein, G.S. et al, Arthritis and Rheumatism 37(5):644 (1994)* 3. Alvaro, J.M. et al, J. Clin. Immunol. 13(3):212 (1993)* 4. Yamanishi, Y. et al, J. Immunol. 168(3):1405 (2002) 5. Firestein, G.S. et al, J. Clin. Invest. 96:1631 (1995) 6. Firestein G.S. et al, Am. J. Pathol. 148(6):2143 (1996) 7. Firestein, G.S. et al, Am. J. Pathol. 148(6):2143 (1996) 8. Rinaldi, N. et al, Ann. Rheum. Dis. 56(12):729 (1997) Each lot is tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, and fungi. Products are for research use only. They are not intended for human, animal, or diagnostic applications. For more product information and a reference list * www.cellapplications.com Follow Cell Applications, Inc. * LinkedIn: www.linkedin.com/company/cell-applications-inc. * Facebook: www.facebook.com/cellapplications * Twitter: witter.com/cellappsinc * Google+: plus.google.com/+Cellapplications