- published: 22 Oct 2013
- views: 1359
In honor of PLOS Biology's 10th anniversary, PLOS Co-Founder Michael Eisen and PLOS Biology Advisory Board Chief Jonathan Eisen discuss the history and future of the journal and Open Access.
Cell Cycle-Dependent Differentiation Dynamics Balances Growth and Endocrine Differentiation in the Pancreas. Yung Hae Kim et al (2015), PLoS Biology http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002111 Organogenesis relies on the spatiotemporal balancing of differentiation and proliferation driven by an expanding pool of progenitor cells. In the mouse pancreas, lineage tracing at the population level has shown that the expanding pancreas progenitors can initially give rise to all endocrine, ductal, and acinar cells but become bipotent by embryonic day 13.5, giving rise to endocrine cells and ductal cells. However, the dynamics of individual progenitors balancing self-renewal and lineage-specific differentiation has never been described. Using three-dimensional live...
Conformational Changes during Pore Formation by the Perforin-Related Protein Pleurotolysin. Natalya Lukoyanova et al (2015), PLoS Biology http://dx.doi.org/10.1371/journal.pbio.1002049 Membrane attack complex/perforin-like (MACPF) proteins comprise the largest superfamily of pore-forming proteins, playing crucial roles in immunity and pathogenesis. Soluble monomers assemble into large transmembrane pores via conformational transitions that remain to be structurally and mechanistically characterised. Here we present an 11 Å resolution cryo-electron microscopy (cryo-EM) structure of the two-part, fungal toxin Pleurotolysin (Ply), together with crystal structures of both components (the lipid binding PlyA protein and the pore-forming MACPF component PlyB). These data reveal a 13-fold pore 80...
Bistability in a Metabolic Network Underpins the De Novo Evolution of Colony Switching in Pseudomonas fluorescens. Jenna Gallie et al (2015), PLoS Biology http://dx.doi.org/10.1371/journal.pbio.1002109 Phenotype switching is commonly observed in nature. This prevalence has allowed the elucidation of a number of underlying molecular mechanisms. However, little is known about how phenotypic switches arise and function in their early evolutionary stages. The first opportunity to provide empirical insight was delivered by an experiment in which populations of the bacterium Pseudomonas fluorescens SBW25 evolved, de novo, the ability to switch between two colony phenotypes. Here we unravel the molecular mechanism behind colony switching, revealing how a single nucleotide change in a gene enmesh...
Composition, Formation, and Regulation of the Cytosolic C-ring, a Dynamic Component of the Type III Secretion Injectisome. Andreas Diepold et al. (2015), PLoS Biology, http://dx.doi.org/10.1371/journal.pbio.1002039 Many gram-negative pathogens employ a type III secretion injectisome to translocate effector proteins into eukaryotic host cells. While the structure of the distal “needle complex” is well documented, the composition and role of the functionally important cytosolic complex remain less well understood. Using functional fluorescent fusions, we found that the C-ring, an essential and conserved cytosolic component of the system, is composed of ~22 copies of SctQ (YscQ in Yersinia enterocolitica), which require the presence of YscQC, the product of an internal translation initiation...
Alpha-catenin-Dependent Recruitment of the Centrosomal Protein CAP350 to Adherens Junctions Allows Epithelial Cells to Acquire a Columnar Shape. Maria P. Gavilan et al (2015), PLoS Biology http://dx.doi.org/10.1371/journal.pbio.1002087 Epithelial morphogenesis involves a dramatic reorganisation of the microtubule cytoskeleton. How this complex process is controlled at the molecular level is still largely unknown. Here, we report that the centrosomal microtubule (MT)-binding protein CAP350 localises at adherens junctions in epithelial cells. By two-hybrid screening, we identified a direct interaction of CAP350 with the adhesion protein α-catenin that was further confirmed by co-immunoprecipitation experiments. Block of epithelial cadherin (E-cadherin)-mediated cell-cell adhesion or α-caten...
A Low-Cost, Hands-on Module to Characterize Antimicrobial Compounds Using an Interdisciplinary, Biophysical Approach. Karishma S. Kaushik et al. (2015), PLoS Biology, http://dx.doi.org/10.1371/journal.pbio.1002044 We have developed a hands-on experimental module that combines biology experiments with a physics-based analytical model in order to characterize antimicrobial compounds. To understand antibiotic resistance, participants perform a disc diffusion assay to test the antimicrobial activity of different compounds and then apply a diffusion-based analytical model to gain insights into the behavior of the active antimicrobial component. In our experience, this module was robust, reproducible, and cost-effective, suggesting that it could be implemented in diverse settings such as underg...
Viruses Roll the Dice: The Stochastic Behavior of Viral Genome Molecules Accelerates Viral Adaptation at the Cell and Tissue Levels. Shuhei Miyashita et al (2015), PLoS Biology http://dx.doi.org/10.1371/journal.pbio.1002094 Recent studies on evolutionarily distant viral groups have shown that the number of viral genomes that establish cell infection after cell-to-cell transmission is unexpectedly small (1–20 genomes). This aspect of viral infection appears to be important for the adaptation and survival of viruses. To clarify how the number of viral genomes that establish cell infection is determined, we developed a simulation model of cell infection for tomato mosaic virus (ToMV), a positive-strand RNA virus. The model showed that stochastic processes that govern the replication or degra...
PLOS Biology PLOS Biology, abgekürzt PLOS Biol., ist eine wissenschaftliche Zeitschrift, die von der Public Library of Science, abgekürzt PLOS veröffentlicht wird.Die erste Ausgabe erschien 2003, sie erscheint monatlich.Veröffentlicht werden Artikel von besonderer Bedeutung und Originalität aus allen Bereichen der biologischen Wissenschaften.Die Zeitschrift gewährt einen offenen Zugang zu allen Artikeln. ✪Video ist an blinde Nutzer gerichtet ✪Text verfügbar unter der Lizens CC-BY-SA ✪Bild Quelle im Video
Noam Chomsky's presentation (and Q&A;) - by videolink - at the Academy Colloquium 'The Biology of Language', Royal Dutch Academy of Sciences (KNAW), Trippenhuis, Amsterdam, Friday 12 Dec 2014. Part 2 of 3 Some of what Chomsky says here about the evolution of language is discussed in more detail in a recent paper in PLoS Biology: Bolhuis, Tattersall, Chomsky & Berwick (2014) How could language have evolved? PLoS Biology, 12(8): e1001934. doi:10.1371/journal.pbio.1001934. Available freely online: http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001934
PLOS Biology is a peer-reviewed scientific journal covering all aspects of Biology.Publication began on October 13, 2003.It was the first journal of the Public Library of Science.PLOS Biology is an Open access journal published under the Creative Commons Attribution license. This channel is dedicated to make Wikipedia, one of the biggest knowledge databases in the world available to people with limited vision. Article available under a Creative Commons license Image source in video
Molecular Dynamics Simulations of the Bacterial UraA H+-Uracil Symporter in Lipid Bilayers Reveal a Closed State and a Selective Interaction with Cardiolipin. Antreas C. Kalli et al (2015), PLoS Computational Biology http://dx.doi.org/10.1371/journal.pcbi.1004123 The Escherichia coli UraA H+-uracil symporter is a member of the nucleobase/ascorbate transporter (NAT) family of proteins, and is responsible for the proton-driven uptake of uracil. Multiscale molecular dynamics simulations of the UraA symporter in phospholipid bilayers consisting of: 1) 1-palmitoyl 2-oleoyl-phosphatidylcholine (POPC); 2) 1-palmitoyl 2-oleoyl-phosphatidylethanolamine (POPE); and 3) a mixture of 75% POPE, 20% 1-palmitoyl 2-oleoyl-phosphatidylglycerol (POPG); and 5% 1-palmitoyl 2-oleoyl-diphosphatidylglycerol/card...
Membrane-Mediated Interaction between Strongly Anisotropic Protein Scaffolds. Yonatan Schweitzer and Michael M. Kozlov (2015), PLoS Computational Biology http://dx.doi.org/10.1371/journal.pcbi.1004054 Specialized proteins serve as scaffolds sculpting strongly curved membranes of intracellular organelles. Effective membrane shaping requires segregation of these proteins into domains and is, therefore, critically dependent on the protein-protein interaction. Interactions mediated by membrane elastic deformations have been extensively analyzed within approximations of large inter-protein distances, small extents of the protein-mediated membrane bending and small deviations of the protein shapes from isotropic spherical segments. At the same time, important classes of the realistic membrane-s...
Statistical Mechanics Provides Novel Insights into Microtubule Stability and Mechanism of Shrinkage. Ishutesh Jain et al (2015), PLoS Computational Biology http://dx.doi.org/10.1371/journal.pcbi.1004099 Microtubules are nano-machines that grow and shrink stochastically, making use of the coupling between chemical kinetics and mechanics of its constituent protofilaments (PFs). We investigate the stability and shrinkage of microtubules taking into account inter-protofilament interactions and bending interactions of intrinsically curved PFs. Computing the free energy as a function of PF tip position, we show that the competition between curvature energy, inter-PF interaction energy and entropy leads to a rich landscape with a series of minima that repeat over a length-scale determined by the...
Noam Chomsky's presentation (and Q&A;) - by videolink - at the Academy Colloquium 'The Biology of Language', Royal Dutch Academy of Sciences (KNAW), Trippenhuis, Amsterdam, Friday 12 Dec 2014. Part 1 of 3 Some of what Chomsky says here about the evolution of language is discussed in more detail in a recent paper in PLoS Biology: Bolhuis, Tattersall, Chomsky & Berwick (2014) How could language have evolved? PLoS Biology, 12(8): e1001934. doi:10.1371/journal.pbio.1001934. Available freely online: http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001934
Noam Chomsky's presentation (and Q&A;) - by videolink - at the Academy Colloquium 'The Biology of Language', Royal Dutch Academy of Sciences (KNAW), Trippenhuis, Amsterdam, Friday 12 Dec 2014. Part 3 of 3 Some of what Chomsky says here about the evolution of language is discussed in more detail in a recent paper in PLoS Biology: Bolhuis, Tattersall, Chomsky & Berwick (2014) How could language have evolved? PLoS Biology, 12(8): e1001934. doi:10.1371/journal.pbio.1001934. Available freely online: http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001934
Is science broken? The scientific method appears sacrosanct to the public but is the image we have of the rational thinker and researcher justified and moreover is it even remotely accurate? Tune in to find out! Links: https://www.youtube.com/channel/UCV1xgnhR6qh08Wc-XSwVelA https://archpedi.jamanetwork.com/article.aspx?articleid=2375127 http://journals.plos.org/plosmedicine/article?id=10.1371/journal.pmed.0020124 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294645/pdf/zii891.pdf http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002547 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479492/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479492/table/t02/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294664/figure/F1/ http://www.simplyblood.org/2016/09/non-scient...
http://genesisweek.com http://christianima.com In this episode: Bacterial antibiotic resistance is again dethrowned as one of the best pieces of evidence for evolution - and takes evolution down with it! We respond to viewer questions about the Green River Formation and the claims of a creepy YouTube atheist about the Ashley Phosphate beds. This is the season finale of Genesis Week, season 4! Random References: Glencoe Biology: Dynamics of Life http://www.glencoe.com/sec/science/biology/bio2004/ Berkeley's "Understanding Evolution" http://evolution.berkeley.edu/evolibrary/article/medicine_03 University of Montana "Teach Evolution and Make it Relevant" http://evoled.dbs.umt.edu/lessons/background.htm Evolution by Any Other Name: Antibiotic Resistance and Avoidance of the ...
What Does it Take to Make Networked and Open Science Sustainable? Ms Catriona MacCallum, Senior Advocacy Manager, Public Library of Science (PLOS), USA The traditional scholarly cycle is being disrupted by interactions between new players, new services and new platforms, resulting in an emerging network of collaborators, communities and innovative forms of scholarly discourse that extend beyond the academy. Taking advantage of this network requires a transformation in the way we think about scholarly communication, and a transformation in the publishing industry to one that is focused on services rather than on products. The internet and Open Access are just the first steps. Both provide opportunities to drive down the fixed costs of traditional publishing and increase the accessibility...
STEM Women hosted a Google+ Hangout On Air with Jonathan Eisen on how men can help with the issues of gender inequality in STEM fields. Jonathan is a Professor at UC Davis and also the Academic Editor-in-Chief for PLOS Biology (http://www.plosbiology.org/). He is a passionate advocate of gender equality in STEM (see his blog http://phylogenomics.blogspot.ie/p/posts-on-women-in-science.html). Jonathan spoke with us about his work with the NSF UC Davis Advance Program which seeks to address academic recruitment, retention and promotion of women (http://www.portal.advance.vt.edu/). He also gave a number of practical tips on what we can do to encourage women in STEM. This is an important issue that affects both men *and* women. Read a text summary of this discussion on our website: http://w...
http://genesisweek.com http://christianima.com In this esipode, Ian discusses the fossil whales found in the driest desert in the world, as well as the allegedly oldest rock crystal found in the world. He then dives into the mailbag with questions about radiodating and speciation. This is Genesis Week! Random References: Repeated mass strandings of Miocene marine mammals from Atacama Region of Chile point to sudden death at sea Nicholas Pyenson, et. al., Proceedings of the Royal Society B, 26 Feb 2014 http://rspb.royalsocietypublishing.org/content/281/1781/20133316 Scientists Solve the Mystery of a Nine-Million-Year-Old Mass Whale Die-Off Joseph Stromberg, SmithsonianMag.com http://www.smithsonianmag.com/science-nature/scientists-solve-mystery-nine-million-year-old-mass-whale-die-off...
"3D Printing Vascularized Tissue for Studies in Pathology and Regenerative Medicine" by Dr Jordan S Miller from the Microphysiological Systems Engineering and Advanced Materials Laboratory in the Rice University Department of Bioengineering. Dr Miller's essay in PLOS Biology was published on the same day this video was released: http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001882