AFM Principle - How AFM Works
To watch the new version of this video has narration,
https://youtu.be/5FsAWr5AwFo?list=PLH4cAUjlEqR0LPdWN_8zMvf_thJCe6SmA
Nano World
- 'Nano', from the
Greek word for 'dwarf', corresponds to a prefix denoting a factor of 10-9. Thus, on nanometer is 10-9m, which is the length scale at which intermolecular forces and quantum effects take hold. To put the nanocale in a more understandable perspective, consider that the size of an atom relative to an apple is similar to the size of an apple relative to the planet
Earth!
Atomic Force Microscopes (AFMs) give us a window into this nanoscale world.
AFM Principle
-
Surface Sensing
An AFM uses a cantilever with a very sharp tip to scan over a sample surface. As the tip approaches the surface, the close-range, attractive forces between the surface and the tip cause the cantilever to deflect towards the surface. However, as the cantilever is brought even closer to the surface, such that the tip is in contact with the surface, increasingly repulsive force take over and cause the cantilever to deflect away from the surface.
- detection
Method
A laser beam is used to detect cantilever deflections towards or away from the surface. By reflecting an incident beam off the flat top of the cantilever, any cantilever deflections will cause slight changes in the direction of the reflected beam. A position-sensitive photodiode (PSPD) can be used to track these changes. Thus, if an AFM tip passes over araised surface feature, the resulting cantilever deflection (and the subsequent change in direction of reflected beam) is recorded by the PSPD.
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Imaging
AFM images the topography of a sample surface by scanning the cantilever over a region of interest. The raised and lowered features on the sample surface influence the deflection of the cantilever, which is monitored by the PSPD. By using a feedback loop to control the height of the tip above the surface-thus maintaining constant laser position - the AFM can generate an accurate topographic map of the surface features.
Learn more about AFM
Modes at
http://www.parkafm.com