Fragment antigen-binding

The fragment antigen-binding (Fab) fragment is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. The variable domain contains the paratope (the antigen-binding site), comprising a set of complementarity determining regions, at the amino terminal end of the monomer. Each arm of the Y thus binds an epitope on the antigen.

In an experimental setting, Fc and Fab fragments can be generated in the laboratory. The enzyme papain can be used to cleave an immunoglobulin monomer into two Fab fragments and an Fc fragment. The enzyme pepsin cleaves below hinge region, so a F(ab')₂ fragment and a pFc' fragment is formed. Recently another enzyme for generation of F(ab')₂ has been commercially available. The enzyme IdeS (Immunoglobulin degrading enzyme from Streptococcus pyogenes, trade name FabRICATOR) cleaves IgG in a sequence specific manner at neutral pH. The F(ab')₂ fragment can be split into two Fab' fragments by mild reduction.

Fab (website)

Fab is an e-commerce company founded in 2010. Once estimated at a worth of over 1 billion dollars, in November 2014, the company was rumored to be in talks to sell for 15 million. In March 2015, PCH International acquired Fab for an undisclosed sum.

It grew from 175,000 members in June 2011, to over 10 million as of December 2012. As of April 30, 2013, Fab has disclosed that it receives 6 million unique visitors per month and have sold over 7 million products since launch. Fab is headquartered in Manhattan and operates a warehouse in Keasbey, New Jersey. The company has office locations in NYC, Pune (India), and Berlin.

History

Fab was founded in February 2010 by Jason Goldberg (entrepreneur) (formerly of XING AG and Jobster) and chief designer Bradford Shellhammer. The site was originally created as a social network for gay men and their friends before pivoting on June 9, 2011 into its model of daily design inspirations and sales. In November 2011, five months after re-launching, Fab reached 1 million members. The company reached this number faster than Facebook, Twitter and Groupon. In September 2012, Fab removed the membership requirements to browse the site. In December 2012, Fab passed 10 million members, up from 7.5 million members in September. Fab also announced it had sold more than 4.3 million products since its launch, averaging 5.4 products sold per minute. In July 2013, fab eliminated 100 positions at its Berlin office as a profit-building measure. Fab laid off 101 more staff, primarily at its New York office in October 2013, further reducing their workforce by 19 percent.

Fab Five

Fab Five may refer to:

See also

Summertime

Summertime may refer to:

Seasons and time of day

Art, entertainment, and media

Film

Literature

Music

Albums

Summertime (The Maybes? song)

"Summertime" is the third single released by The Maybes? from their debut album, Promise. It was released on 25 August 2008 on Xtra Mile Recordings as a download and 7" Record.

Track listing

Download Single

7" Single

Personnel

References

External links

Charles L. Mee

Charles L. Mee (born September 15, 1938) is an American playwright, historian and author known for his collage-like style of playwriting, which makes use of radical reconstructions of found texts. He is also a professor of theater at Columbia University.

Early life and career

Mee was born in Evanston, Illinois, in 1938. He contracted polio at the age of fourteen. His memoir A Nearly Normal Life (1999) tells how that event informed the rest of his life.

After graduating from Harvard University in 1960, Mee moved to Greenwich Village and became a part of the Off-Off-Broadway scene. Between 1962 and 1964, his plays were presented at venues that included La MaMa E.T.C., Caffe Cino, Theatre Genesis, and the Ontological-Hysteric Theater.

In 1961 Mee began work at American Heritage publishing company and eventually became the editor of the hardback bi-monthly Horizon: A Magazine of the Arts. He was also the Advising Editor and then Contributing Editor of Tulane Drama Review – now called TDR and published from New York University – until 1964 and its Associate Editor from 1964 to 1965.