A thermocline (sometimes metalimnion) is a thin but distinct layer in a large body of fluid (e.g. water, such as an ocean or lake, or air, such as an atmosphere), in which temperature changes more rapidly with depth than it does in the layers above or below. In the ocean, the thermocline may be thought of as an invisible blanket which separates the upper mixed layer from the calm deep water below. Depending largely on season, latitude and turbulent mixing by wind, thermoclines may be a semi-permanent feature of the body of water in which they occur, or they may form temporarily in response to phenomena such as the radiative heating/cooling of surface water during the day/night. Factors that affect the depth and thickness of a thermocline include seasonal weather variations, latitude, and local environmental conditions, such as tides and currents.

Most of the heat energy of sunlight is absorbed in the first few centimeters at the ocean's surface, which heats during the day and cools at night as heat energy is lost to space by radiation. Waves mix the water near the surface layer and distribute heat to deeper water, such that the temperature may be relatively uniform in the upper 100 m (300 ft), depending on wave strength and the existence of surface turbulence caused by currents. Below this mixed layer, the temperature remains relatively stable over day/night cycles. The temperature of the deep ocean drops gradually with depth. As saline water does not freeze until it reaches −2.3 °C (colder as depth and pressure increase) the temperature well below the surface is usually not far from zero degrees.