Troponin I, fast skeletal muscle is a protein that in humans is encoded by the TNNI2 gene.
The TNNI2 gene is located at 11p15.5 in the human chromosomal genome, encoding the fast twitch skeletal muscle troponin I (fsTnI). fsTnI is a 21.3 kDa protein consisting of 182 amino acids including the first methionine with an isoelectric point (pI) of 8.74. It is the inhibitory subunit of the troponin complex in fast twitch skeletal muscle fibers.
Three homologous genes have evolved in vertebrates, encoding three muscle type-specific isoforms of TnI. Sequence analysis, immunological distance, and examination of evolutionarily suppressed conformational states showed that the TnI genes have evolved in close linkage with the genes encoding troponin T (TnT), another subunit of the troponin complex. The fast TnI-fast TnT gene pair represents the original TnI and TnT genes (Fig. 1). The three muscle fiber type-specific TnI-TnT gene pairs were likely originated from a TnI-like ancestor gene that presumably duplicated to form a closely linked fast TnI-like and fast TnT-like gene pair. A later duplication events resulted in emergences of a slow TnI-like and cardiac TnT-like gene pair that was further duplicated to give rise of the present-day slow TnI-cardiac TnT and cardiac TnI-slow TnT gene pairs. The seemingly scrambled ssTnI and cTnI gene pair is actually functionally related as they co-express and form troponin complex in the embryonic heart. The overlapping of enhancer elements of the TnT gene promoter with the upstream TnI gene structure may be a critical factor in the preservation of the close linkage of TnI and TnT gene pairs
Ayy ayy ayy ok ok juicy gucci juicy gucci half a brick, half a brick, zaytoven, zaytiggi, ayy ayy ayy ok ok ok ayy ayy so icey entertainment
[Over intro: Tony Yayo]
Yea, half a brick, yeaaaa
G-unit
Ayyy, ayyy, okkk, ayyyy
Yea, g-unit
[Chorus: x2]
Quarter Brick, half a brick,
Whole brick (Aaay!)
Quarter pound, half a pound,
Whole pound (Okay!)
100 pillz, 1000 pillz, servin major
Weight
JuiceMan and Gucci Mane make the trap
[Tony Yayo:]
Quarter brick, half a brick, whole key and yay
Got me in that louie, gucci and that hermeez
200 thousand that's 3 bricks a day
170 real cats the other 30's like flake
I'm an og in the a, looking fresh to death
I gotta thousand e pills and that crystal meth
I got that rose breath, I got them dior feet
And we lookin like money on bolter cresh street
Yeaaaaaa
[Chorus x2]
[OJ Da Juiceman:]
I'm boomin I'm buggin I'm termin all the
Baites
Rap game ezy but da dope game gravy
Young juice man and my life is the
Japerz
With stupid fruity crazy swag jumpin in
Yo lader
Banana donk chevy interior like the
Lakers
Lebron james wrist when I'm fuckin with
Yaper
Hit the trap, stay down watch the paper
Wake up
Boomin out da house and J askin for a
Waiter
Half a brick whole brick got me buyin jacob
Worried still walking in a head of a gator
Half a brick whole brick got me buyin jacob
Worried still walking in a head of a gator
[Chorus x2]
[Gucci Mane:]
I'm twerkin birds in so we workin
Packin a truck stop to train a back in
We big flip jug we tote it off the
Forklift
The way my plug kick ya think he
Had a black belt
My scale so big big boy can weight
It's damn self
2000 pounds of mid I sold dat shit my
Damn self
Washer full of cash dryer full of X
Pillz
Red rag in my pocket same color my
Vette is
My number lower than a ese from texas a
Quarter mil in da mail is an investment
A sniper rifle like a soldier in the
Desert
A eagle on me boy I'm known to tote a
Desert
I sack a ounce up before I sold a
Record
He won't a brick I told him meet me by
The checkers
I sack a pound up before I sold a
Record
He want a bet I told him meet me by
The checkers
[Chorus x2]
[OJ Da Juiceman:]
Young juice man God dammit I'm the shit
Boomin on the chain working with 50 bricks
1000 pound bail tryna make a mega grill
Posted on the cresh with that 4.5 on my hip
34, 34 tuck behind my heel
Tell 60 dawg with that extra limb
502 dark dawg with thate extra kid
I got that stupid bank cause I got that stupid wheel
[Chorus x2]
Ayy ayy ayy ok
Damn damn say say
Dam damn damn juice juice