-
A Senior PLOS Editor Speaks! Advice on Getting Published in PLOS One......
8/28/13 - A Senior PLOS Editor Speaks! Advice on Getting Published in PLOS One and Observations about Trends in Publishing Speaker: Elizabeth Silva, M.Sc. Ph...
-
Martin Solveig « +1 » (feat. Sam White) [Official Video]
Martin Solveig « +1 » (feat. Sam White) is OUT NOW! Grab your copy on Beatport HERE: http://btprt.dj/1HhXDPV
Subscribe to Spinnin' TV now : http://bit.ly/SPINNINTV
Catchy stuff as ever from the mighty Martin Solveig. The Frenchman delivers a funked up house groove, incorporating feelgood pop vocals and steady basslines lifting you up as the melody gets stronger. Always good to have a +1, this o
-
Part 1 - Editing and Publishing in PLoS One Journal
Talk given by J. David Creswell, a professor in the Psychology department, on editing and publishing in PLoS One journals. Part 1 of the "Open Scholarship fo...
-
in PLOS - parody of Lady Gaga's "Applause"
A tale about the pressure to publish in fashionable, high-impact journals and the relief that can be found by publishing in an open-access journal such as PL...
-
PLOS ONE Structured Review Form
We are excited to announce a new review form for PLOS ONE. The form focuses the review process on our editorial criteria.
-
PLoS ONE : Scorpion Sheds ‘Tail’ to Escape: Consequences and Implications of Autotomy...
Scorpion Sheds ‘Tail’ to Escape: Consequences and Implications of Autotomy in Scorpions (Buthidae: Ananteris). Camilo I. Mattoni et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0116639
Autotomy, the voluntary shedding or detachment of a body part at a determined cleavage plane, is a common anti-predation defense mechanism in several animal taxa, including arthropods. Among arachnid
-
PLoS ONE : Live Imaging of Companion Cells and Sieve Elements in Arabidopsis Leaves
Live Imaging of Companion Cells and Sieve Elements in Arabidopsis Leaves. Thibaud Cayla et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118122
The phloem is a complex tissue composed of highly specialized cells with unique subcellular structures and a compact organization that is challenging to study in vivo at cellular resolution. We used confocal scanning laser microscopy and sub
-
Los - One And Only (ft. Vicente)
Subscribe to SwagyTracks for more music daily !
http://bit.ly/SubscribeSwagy
.........
• Los -
http://www.youtube.com/CherfinMusic
https://www.facebook.com/CherfinMusic
Download this track •
http://snd.sc/1dJPR0j
Picture credit •
http://www.flickr.com/photos/oscarjones/6881719510/sizes/k/in/gallery-isaac_rangel-72157629498080810/
If you are the owner of this picture and want it removed,contact
-
Terror Bird - animation of the bite of Andalgalornis based on PLoS ONE research
Research on the anatomy and feeding mechanics of extinct phorusrhacids ("terror birds") was published in an article in PLoS ONE (http://bit.ly/aQr9ND). The a...
-
PLoS ONE : Comparison of Human Induced Pluripotent Stem-Cell Derived Cardiomyocytes with Human...
KeSimpulan | Comparison of Human Induced Pluripotent Stem-Cell Derived Cardiomyocytes with Human Mesenchymal Stem Cells following Acute Myocardial Infarction. Lucas Citro et al. (2014), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0116281
-
PLoS ONE : Inferior Cerebellar Hypoplasia Resembling a Dandy-Walker-Like Malformation in Purebred...
Inferior Cerebellar Hypoplasia Resembling a Dandy-Walker-Like Malformation in Purebred Eurasier Dogs with Familial Non-Progressive Ataxia: A Retrospective and Prospective Clinical Cohort Study. Filipa Bernardino et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0117670
Cerebellar malformations can be inherited or caused by insults during cerebellar development. To date, only sporadic
-
PLoS ONE : Differentiation of Apical and Basal Dendrites in Pyramidal Cells and Granule Cells...
Differentiation of Apical and Basal Dendrites in Pyramidal Cells and Granule Cells in Dissociated Hippocampal Cultures. You Kure Wu et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118482
Hippocampal pyramidal cells and dentate granule cells develop morphologically distinct dendritic arbors, yet also share some common features. Both cell types form a long apical dendrite which exten
-
PLoS ONE : Posture Affects How Robots and Infants Map Words to Objects
Posture Affects How Robots and Infants Map Words to Objects. Anthony F. Morse et al (2015), PLoS ONE http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116012
For infants, the first problem in learning a word is to map the word to its referent; a second problem is to remember that mapping when the word and/or referent are again encountered. Recent infant studies suggest that spatia
-
PLoS ONE : Rapid Screening of Gene Function by Systemic Delivery of Morpholino Oligonucleotides...
Rapid Screening of Gene Function by Systemic Delivery of Morpholino Oligonucleotides to Live Mouse Embryos. Kathryn S. McClelland et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0114932
Traditional gene targeting methods in mice are complex and time consuming, especially when conditional deletion methods are required. Here, we describe a novel technique for assessing gene function
-
PLoS ONE : Structure, Dynamics, and Interaction of Mycobacterium tuberculosis (Mtb) DprE1...
Structure, Dynamics, and Interaction of Mycobacterium tuberculosis (Mtb) DprE1 and DprE2 Examined by Molecular Modeling, Simulation, and Electrostatic Studies. Isha Bhutani et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0119771
The enzymes decaprenylphosphoryl-β-D-ribose oxidase (DprE1) and decaprenylphosphoryl-β-D-ribose-2-epimerase(DprE2) catalyze epimerization of decaprenylphos
-
PLoS ONE : Pain Inhibition by Optogenetic Activation of Specific Anterior Cingulate Cortical Neurons
Pain Inhibition by Optogenetic Activation of Specific Anterior Cingulate Cortical Neurons. Ling Gu et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0117746
Cumulative evidence from both humans and animals suggests that the anterior cingulate cortex (ACC) is important for pain-related perception, and thus a likely target for pain relief therapy. However, use of existing electrode base
-
PLoS ONE : Drinking Songs: Alcohol Effects on Learned Song of Zebra Finches
KeSimpulan | Drinking Songs: Alcohol Effects on Learned Song of Zebra Finches. Christopher R. Olson et al. (2014), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0115427
-
PLoS ONE: Multiple Requirements of PLK1 during Mouse Oocyte Maturation
Multiple Requirements of PLK1 during Mouse Oocyte Maturation. Petr Solc et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0116783
Polo-like kinase 1 (PLK1) orchestrates multiple events of cell division. Although PLK1 function has been intensively studied in centriole-containing and rapidly cycling somatic cells, much less is known about its function in the meiotic divisions of mammali
-
Plos One: Skorpion smider halen - og anus - under flugtforsøg
Video fra Plos One viser, hvordan visse skorpioner er i stand til at smide hale og anus for at flygte. Skorpionerne helede hurtigt og levede op til otte måneder efter i fangenskab.
-
PLoS ONE : In Vivo Imaging and Characterization of Actin Microridges
In Vivo Imaging and Characterization of Actin Microridges. Pui-ying Lam et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0115639
Actin microridges form labyrinth like patterns on superficial epithelial cells across animal species. This highly organized assembly has been implicated in mucus retention and in the mechanical structure of mucosal surfaces, however the mechanisms that reg
-
PLoS ONE : Function and Evolutionary Origin of Unicellular Camera-Type Eye Structure
Function and Evolutionary Origin of Unicellular Camera-Type Eye Structure. Shiho Hayakawa et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118415
The ocelloid is an extraordinary eyespot organelle found only in the dinoflagellate family Warnowiaceae. It contains retina- and lens-like structures called the retinal body and the hyalosome. The ocelloid has been an evolutionary enigma b
-
Plos One y la dilatación de las pupilas.
Maricuajis: la semana pasada tuvimos problemas para subir el vídeo, pero ya estamos aquí otra vez!!! Bechotos!!! ^oo^. Más ...
-
PLoS ONE : How Do “Mute” Cicadas Produce Their Calling Songs?
How Do “Mute” Cicadas Produce Their Calling Songs? Changqing Luo et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118554
Insects have evolved a variety of structures and mechanisms to produce sounds, which are used for communication both within and between species. Among acoustic insects, cicada males are particularly known for their loud and diverse sounds which function importantl
A Senior PLOS Editor Speaks! Advice on Getting Published in PLOS One......
8/28/13 - A Senior PLOS Editor Speaks! Advice on Getting Published in PLOS One and Observations about Trends in Publishing Speaker: Elizabeth Silva, M.Sc. Ph......
8/28/13 - A Senior PLOS Editor Speaks! Advice on Getting Published in PLOS One and Observations about Trends in Publishing Speaker: Elizabeth Silva, M.Sc. Ph...
wn.com/A Senior Plos Editor Speaks Advice On Getting Published In Plos One......
8/28/13 - A Senior PLOS Editor Speaks! Advice on Getting Published in PLOS One and Observations about Trends in Publishing Speaker: Elizabeth Silva, M.Sc. Ph...
Martin Solveig « +1 » (feat. Sam White) [Official Video]
Martin Solveig « +1 » (feat. Sam White) is OUT NOW! Grab your copy on Beatport HERE: http://btprt.dj/1HhXDPV
Subscribe to Spinnin' TV now : http://bit.ly/SPIN...
Martin Solveig « +1 » (feat. Sam White) is OUT NOW! Grab your copy on Beatport HERE: http://btprt.dj/1HhXDPV
Subscribe to Spinnin' TV now : http://bit.ly/SPINNINTV
Catchy stuff as ever from the mighty Martin Solveig. The Frenchman delivers a funked up house groove, incorporating feelgood pop vocals and steady basslines lifting you up as the melody gets stronger. Always good to have a +1, this one will surely get the party started.
Video director : Martin Solveig
Executive producer : Benjamin Benhamou for Monsieur L’Agent (Paris)
Artistic supervision : Julien Drapier, Nathanael Day
Director of photography : Jacques Ballard
First assistant director : Alexis de la Mure
Editing and special effects : Birdy Ben for El Motion Lab (Paris)
Editing : Christian Georges
Color grading : Arthur Paux
Art Departement : Sebastien Cachon
Style : Lucille D
Choreographer : Arthur Harel & Jonathan Debrouwer
Dancer : Antoinette Gomis aka Krazy Lock
Dancer : Ylva Kalf
Dancer : Taylor Katle
Dancer : Abdoulaye Barry
Model : Aliane Uwimana
Model : Julia Karolina Johansen
Model : Yassine Chekkouh
Special thanks : Ludivine Gutierrez and Yeoram Kalfa
wn.com/Martin Solveig « 1 » (Feat. Sam White) Official Video
Martin Solveig « +1 » (feat. Sam White) is OUT NOW! Grab your copy on Beatport HERE: http://btprt.dj/1HhXDPV
Subscribe to Spinnin' TV now : http://bit.ly/SPINNINTV
Catchy stuff as ever from the mighty Martin Solveig. The Frenchman delivers a funked up house groove, incorporating feelgood pop vocals and steady basslines lifting you up as the melody gets stronger. Always good to have a +1, this one will surely get the party started.
Video director : Martin Solveig
Executive producer : Benjamin Benhamou for Monsieur L’Agent (Paris)
Artistic supervision : Julien Drapier, Nathanael Day
Director of photography : Jacques Ballard
First assistant director : Alexis de la Mure
Editing and special effects : Birdy Ben for El Motion Lab (Paris)
Editing : Christian Georges
Color grading : Arthur Paux
Art Departement : Sebastien Cachon
Style : Lucille D
Choreographer : Arthur Harel & Jonathan Debrouwer
Dancer : Antoinette Gomis aka Krazy Lock
Dancer : Ylva Kalf
Dancer : Taylor Katle
Dancer : Abdoulaye Barry
Model : Aliane Uwimana
Model : Julia Karolina Johansen
Model : Yassine Chekkouh
Special thanks : Ludivine Gutierrez and Yeoram Kalfa
- published: 08 Jun 2015
- views: 301
Part 1 - Editing and Publishing in PLoS One Journal
Talk given by J. David Creswell, a professor in the Psychology department, on editing and publishing in PLoS One journals. Part 1 of the "Open Scholarship fo......
Talk given by J. David Creswell, a professor in the Psychology department, on editing and publishing in PLoS One journals. Part 1 of the "Open Scholarship fo...
wn.com/Part 1 Editing And Publishing In Plos One Journal
Talk given by J. David Creswell, a professor in the Psychology department, on editing and publishing in PLoS One journals. Part 1 of the "Open Scholarship fo...
in PLOS - parody of Lady Gaga's "Applause"
A tale about the pressure to publish in fashionable, high-impact journals and the relief that can be found by publishing in an open-access journal such as PL......
A tale about the pressure to publish in fashionable, high-impact journals and the relief that can be found by publishing in an open-access journal such as PL...
wn.com/In Plos Parody Of Lady Gaga's Applause
A tale about the pressure to publish in fashionable, high-impact journals and the relief that can be found by publishing in an open-access journal such as PL...
PLOS ONE Structured Review Form
We are excited to announce a new review form for PLOS ONE. The form focuses the review process on our editorial criteria....
We are excited to announce a new review form for PLOS ONE. The form focuses the review process on our editorial criteria.
wn.com/Plos One Structured Review Form
We are excited to announce a new review form for PLOS ONE. The form focuses the review process on our editorial criteria.
- published: 13 Dec 2012
- views: 25944
-
author: PLOS Media
PLoS ONE : Scorpion Sheds ‘Tail’ to Escape: Consequences and Implications of Autotomy...
Scorpion Sheds ‘Tail’ to Escape: Consequences and Implications of Autotomy in Scorpions (Buthidae: Ananteris). Camilo I. Mattoni et al (2015), PLoS ONE, http://...
Scorpion Sheds ‘Tail’ to Escape: Consequences and Implications of Autotomy in Scorpions (Buthidae: Ananteris). Camilo I. Mattoni et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0116639
Autotomy, the voluntary shedding or detachment of a body part at a determined cleavage plane, is a common anti-predation defense mechanism in several animal taxa, including arthropods. Among arachnids, autotomy has been observed in harvestmen, mites, and spiders, always involving the loss of legs. Autotomy of the opisthosoma (abdomen) was recently reported in a single species of the Neotropical buthid scorpion genus Ananteris Thorell, 1891, but few details were revealed. Based on observations in the field and laboratory, examination of material in museum collections, and scanning electron microscopy, we document autotomy of the metasoma (the hind part of the opisthosoma, or ‘tail’) in fourteen species of Ananteris. Autotomy is more common in males than females, and has not been observed in juveniles. When the scorpion is held by the metasoma, it is voluntarily severed at the joints between metasomal segments I and II, II and III, or III and IV, allowing the scorpion to escape. After detachment, the severed metasoma moves (twitches) automatically, much like the severed tail of a lizard or the severed leg of a spider, and reacts to contact, even attempting to sting. The severed surface heals rapidly, scar tissue forming in five days. The lost metasomal segments and telson cannot be regenerated. Autotomy of the metasoma and telson results in permanent loss of the posterior part of the scorpion’s digestive system (the anus is situated posteriorly on metasomal segment V) and the ability to inject venom by stinging. After autotomy, scorpions do not defecate and can only capture small prey items. However, males can survive and mate successfully for up to eight months in the laboratory. In spite of diminished predation ability after autotomy, survival allows males to reproduce. Autotomy in Ananteris therefore appears to be an effective, adaptive, anti-predation escape mechanism.
wn.com/Plos One Scorpion Sheds ‘Tail’ To Escape Consequences And Implications Of Autotomy...
Scorpion Sheds ‘Tail’ to Escape: Consequences and Implications of Autotomy in Scorpions (Buthidae: Ananteris). Camilo I. Mattoni et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0116639
Autotomy, the voluntary shedding or detachment of a body part at a determined cleavage plane, is a common anti-predation defense mechanism in several animal taxa, including arthropods. Among arachnids, autotomy has been observed in harvestmen, mites, and spiders, always involving the loss of legs. Autotomy of the opisthosoma (abdomen) was recently reported in a single species of the Neotropical buthid scorpion genus Ananteris Thorell, 1891, but few details were revealed. Based on observations in the field and laboratory, examination of material in museum collections, and scanning electron microscopy, we document autotomy of the metasoma (the hind part of the opisthosoma, or ‘tail’) in fourteen species of Ananteris. Autotomy is more common in males than females, and has not been observed in juveniles. When the scorpion is held by the metasoma, it is voluntarily severed at the joints between metasomal segments I and II, II and III, or III and IV, allowing the scorpion to escape. After detachment, the severed metasoma moves (twitches) automatically, much like the severed tail of a lizard or the severed leg of a spider, and reacts to contact, even attempting to sting. The severed surface heals rapidly, scar tissue forming in five days. The lost metasomal segments and telson cannot be regenerated. Autotomy of the metasoma and telson results in permanent loss of the posterior part of the scorpion’s digestive system (the anus is situated posteriorly on metasomal segment V) and the ability to inject venom by stinging. After autotomy, scorpions do not defecate and can only capture small prey items. However, males can survive and mate successfully for up to eight months in the laboratory. In spite of diminished predation ability after autotomy, survival allows males to reproduce. Autotomy in Ananteris therefore appears to be an effective, adaptive, anti-predation escape mechanism.
- published: 29 Jan 2015
- views: 24
PLoS ONE : Live Imaging of Companion Cells and Sieve Elements in Arabidopsis Leaves
Live Imaging of Companion Cells and Sieve Elements in Arabidopsis Leaves. Thibaud Cayla et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118122
T...
Live Imaging of Companion Cells and Sieve Elements in Arabidopsis Leaves. Thibaud Cayla et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118122
The phloem is a complex tissue composed of highly specialized cells with unique subcellular structures and a compact organization that is challenging to study in vivo at cellular resolution. We used confocal scanning laser microscopy and subcellular fluorescent markers in companion cells and sieve elements, for live imaging of the phloem in Arabidopsis leaves. This approach provided a simple framework for identifying phloem cell types unambiguously. It highlighted the compactness of the meshed network of organelles within companion cells. By contrast, within the sieve elements, unknown bodies were observed in association with the PP2-A1:GFP, GFP:RTM1 and RTM2:GFP markers at the cell periphery. The phloem lectin PP2-A1:GFP marker was found in the parietal ground matrix. Its location differed from that of the P-protein filaments, which were visualized with SEOR1:GFP and SEOR2:GFP. PP2-A1:GFP surrounded two types of bodies, one of which was identified as mitochondria. This location suggested that it was embedded within the sieve element clamps, specific structures that may fix the organelles to each another or to the plasma membrane in the sieve tubes. GFP:RTM1 was associated with a class of larger bodies, potentially corresponding to plastids. PP2-A1:GFP was soluble in the cytosol of immature sieve elements. The changes in its subcellular localization during differentiation provide an in vivo blueprint for monitoring this process. The subcellular features obtained with these companion cell and sieve element markers can be used as landmarks for exploring the organization and dynamics of phloem cells in vivo.
wn.com/Plos One Live Imaging Of Companion Cells And Sieve Elements In Arabidopsis Leaves
Live Imaging of Companion Cells and Sieve Elements in Arabidopsis Leaves. Thibaud Cayla et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118122
The phloem is a complex tissue composed of highly specialized cells with unique subcellular structures and a compact organization that is challenging to study in vivo at cellular resolution. We used confocal scanning laser microscopy and subcellular fluorescent markers in companion cells and sieve elements, for live imaging of the phloem in Arabidopsis leaves. This approach provided a simple framework for identifying phloem cell types unambiguously. It highlighted the compactness of the meshed network of organelles within companion cells. By contrast, within the sieve elements, unknown bodies were observed in association with the PP2-A1:GFP, GFP:RTM1 and RTM2:GFP markers at the cell periphery. The phloem lectin PP2-A1:GFP marker was found in the parietal ground matrix. Its location differed from that of the P-protein filaments, which were visualized with SEOR1:GFP and SEOR2:GFP. PP2-A1:GFP surrounded two types of bodies, one of which was identified as mitochondria. This location suggested that it was embedded within the sieve element clamps, specific structures that may fix the organelles to each another or to the plasma membrane in the sieve tubes. GFP:RTM1 was associated with a class of larger bodies, potentially corresponding to plastids. PP2-A1:GFP was soluble in the cytosol of immature sieve elements. The changes in its subcellular localization during differentiation provide an in vivo blueprint for monitoring this process. The subcellular features obtained with these companion cell and sieve element markers can be used as landmarks for exploring the organization and dynamics of phloem cells in vivo.
- published: 25 Feb 2015
- views: 9
Los - One And Only (ft. Vicente)
Subscribe to SwagyTracks for more music daily !
http://bit.ly/SubscribeSwagy
.........
• Los -
http://www.youtube.com/CherfinMusic
https://www.facebook.com/Ch...
Subscribe to SwagyTracks for more music daily !
http://bit.ly/SubscribeSwagy
.........
• Los -
http://www.youtube.com/CherfinMusic
https://www.facebook.com/CherfinMusic
Download this track •
http://snd.sc/1dJPR0j
Picture credit •
http://www.flickr.com/photos/oscarjones/6881719510/sizes/k/in/gallery-isaac_rangel-72157629498080810/
If you are the owner of this picture and want it removed,contact me and I will delete the video as soon as possible,no drama!
.........
Secondary music channel •
http://www.youtube.com/VibeySounds
Follow SwagyTracks on
Facebook • http://www.facebook.com/SwagyTracks
Twitter • https://twitter.com/SwagyTracks
SoundCloud • https://soundcloud.com/SwagyTracks
Instagram • http://instagram.com/swagytracks
Tumblr • http://swagytracks.tumblr.com
.........
There is no copyright infringement intended for the song or picture. If you have an issue with me posting this song or picture please contact me through one of my social networks or YouTube private messaging system. Once I have received your message and determined you are the proper owner of this content I will have it removed, no drama at all. This channel is strictly for promotion towards the artists of the music. I try to help promote their music and their social networks.
wn.com/Los One And Only (Ft. Vicente)
Subscribe to SwagyTracks for more music daily !
http://bit.ly/SubscribeSwagy
.........
• Los -
http://www.youtube.com/CherfinMusic
https://www.facebook.com/CherfinMusic
Download this track •
http://snd.sc/1dJPR0j
Picture credit •
http://www.flickr.com/photos/oscarjones/6881719510/sizes/k/in/gallery-isaac_rangel-72157629498080810/
If you are the owner of this picture and want it removed,contact me and I will delete the video as soon as possible,no drama!
.........
Secondary music channel •
http://www.youtube.com/VibeySounds
Follow SwagyTracks on
Facebook • http://www.facebook.com/SwagyTracks
Twitter • https://twitter.com/SwagyTracks
SoundCloud • https://soundcloud.com/SwagyTracks
Instagram • http://instagram.com/swagytracks
Tumblr • http://swagytracks.tumblr.com
.........
There is no copyright infringement intended for the song or picture. If you have an issue with me posting this song or picture please contact me through one of my social networks or YouTube private messaging system. Once I have received your message and determined you are the proper owner of this content I will have it removed, no drama at all. This channel is strictly for promotion towards the artists of the music. I try to help promote their music and their social networks.
- published: 25 Sep 2013
- views: 13414
Terror Bird - animation of the bite of Andalgalornis based on PLoS ONE research
Research on the anatomy and feeding mechanics of extinct phorusrhacids ("terror birds") was published in an article in PLoS ONE (http://bit.ly/aQr9ND). The a......
Research on the anatomy and feeding mechanics of extinct phorusrhacids ("terror birds") was published in an article in PLoS ONE (http://bit.ly/aQr9ND). The a...
wn.com/Terror Bird Animation Of The Bite Of Andalgalornis Based On Plos One Research
Research on the anatomy and feeding mechanics of extinct phorusrhacids ("terror birds") was published in an article in PLoS ONE (http://bit.ly/aQr9ND). The a...
- published: 18 Aug 2010
- views: 54164
-
author: WitmerLab
PLoS ONE : Comparison of Human Induced Pluripotent Stem-Cell Derived Cardiomyocytes with Human...
KeSimpulan | Comparison of Human Induced Pluripotent Stem-Cell Derived Cardiomyocytes with Human Mesenchymal Stem Cells following Acute Myocardial Infarction. L...
KeSimpulan | Comparison of Human Induced Pluripotent Stem-Cell Derived Cardiomyocytes with Human Mesenchymal Stem Cells following Acute Myocardial Infarction. Lucas Citro et al. (2014), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0116281
wn.com/Plos One Comparison Of Human Induced Pluripotent Stem Cell Derived Cardiomyocytes With Human...
KeSimpulan | Comparison of Human Induced Pluripotent Stem-Cell Derived Cardiomyocytes with Human Mesenchymal Stem Cells following Acute Myocardial Infarction. Lucas Citro et al. (2014), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0116281
- published: 01 Jan 2015
- views: 0
PLoS ONE : Inferior Cerebellar Hypoplasia Resembling a Dandy-Walker-Like Malformation in Purebred...
Inferior Cerebellar Hypoplasia Resembling a Dandy-Walker-Like Malformation in Purebred Eurasier Dogs with Familial Non-Progressive Ataxia: A Retrospective and P...
Inferior Cerebellar Hypoplasia Resembling a Dandy-Walker-Like Malformation in Purebred Eurasier Dogs with Familial Non-Progressive Ataxia: A Retrospective and Prospective Clinical Cohort Study. Filipa Bernardino et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0117670
Cerebellar malformations can be inherited or caused by insults during cerebellar development. To date, only sporadic cases of cerebellar malformations have been reported in dogs, and the genetic background has remained obscure. Therefore, this study`s objective was to describe the clinical characteristics, imaging features and pedigree data of a familial cerebellar hypoplasia in purebred Eurasier dogs. A uniform cerebellar malformation characterized by consistent absence of the caudal portions of the cerebellar vermis and, to a lesser degree, the caudal portions of the cerebellar hemispheres in association with large retrocerebellar fluid accumulations was recognized in 14 closely related Eurasier dogs. Hydrocephalus was an additional feature in some dogs. All dogs displayed non-progressive ataxia, which had already been noted when the dogs were 5 – 6 weeks old. The severity of the ataxia varied between dogs, from mild truncal sway, subtle dysmetric gait, dysequilibrium and pelvic limb ataxia to severe cerebellar ataxia in puppies and episodic falling or rolling. Follow-up examinations in adult dogs showed improvement of the cerebellar ataxia and a still absent menace response. Epileptic seizures occurred in some dogs. The association of partial vermis agenesis with an enlarged fourth ventricle and an enlarged caudal (posterior) fossa resembled a Dandy-Walker-like malformation in some dogs. Pedigree analyses were consistent with autosomal recessive inheritance.
wn.com/Plos One Inferior Cerebellar Hypoplasia Resembling A Dandy Walker Like Malformation In Purebred...
Inferior Cerebellar Hypoplasia Resembling a Dandy-Walker-Like Malformation in Purebred Eurasier Dogs with Familial Non-Progressive Ataxia: A Retrospective and Prospective Clinical Cohort Study. Filipa Bernardino et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0117670
Cerebellar malformations can be inherited or caused by insults during cerebellar development. To date, only sporadic cases of cerebellar malformations have been reported in dogs, and the genetic background has remained obscure. Therefore, this study`s objective was to describe the clinical characteristics, imaging features and pedigree data of a familial cerebellar hypoplasia in purebred Eurasier dogs. A uniform cerebellar malformation characterized by consistent absence of the caudal portions of the cerebellar vermis and, to a lesser degree, the caudal portions of the cerebellar hemispheres in association with large retrocerebellar fluid accumulations was recognized in 14 closely related Eurasier dogs. Hydrocephalus was an additional feature in some dogs. All dogs displayed non-progressive ataxia, which had already been noted when the dogs were 5 – 6 weeks old. The severity of the ataxia varied between dogs, from mild truncal sway, subtle dysmetric gait, dysequilibrium and pelvic limb ataxia to severe cerebellar ataxia in puppies and episodic falling or rolling. Follow-up examinations in adult dogs showed improvement of the cerebellar ataxia and a still absent menace response. Epileptic seizures occurred in some dogs. The association of partial vermis agenesis with an enlarged fourth ventricle and an enlarged caudal (posterior) fossa resembled a Dandy-Walker-like malformation in some dogs. Pedigree analyses were consistent with autosomal recessive inheritance.
- published: 11 Feb 2015
- views: 71
PLoS ONE : Differentiation of Apical and Basal Dendrites in Pyramidal Cells and Granule Cells...
Differentiation of Apical and Basal Dendrites in Pyramidal Cells and Granule Cells in Dissociated Hippocampal Cultures. You Kure Wu et al (2015), PLoS ONE http:...
Differentiation of Apical and Basal Dendrites in Pyramidal Cells and Granule Cells in Dissociated Hippocampal Cultures. You Kure Wu et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118482
Hippocampal pyramidal cells and dentate granule cells develop morphologically distinct dendritic arbors, yet also share some common features. Both cell types form a long apical dendrite which extends from the apex of the cell soma, while short basal dendrites are developed only in pyramidal cells. Using quantitative morphometric analyses of mouse hippocampal cultures, we evaluated the differences in dendritic arborization patterns between pyramidal and granule cells. Furthermore, we observed and described the final apical dendrite determination during dendritic polarization by time-lapse imaging. Pyramidal and granule cells in culture exhibited similar dendritic patterns with a single principal dendrite and several minor dendrites so that the cell types were not readily distinguished by appearance. While basal dendrites in granule cells are normally degraded by adulthood in vivo, cultured granule cells retained their minor dendrites. Asymmetric growth of a single principal dendrite harboring the Golgi was observed in both cell types soon after the onset of dendritic growth. Time-lapse imaging revealed that up until the second week in culture, final principal dendrite designation was not stabilized, but was frequently replaced by other minor dendrites. Before dendritic polarity was stabilized, the Golgi moved dynamically within the soma and was repeatedly repositioned at newly emerging principal dendrites. Our results suggest that polarized growth of the apical dendrite is regulated by cell intrinsic programs, while regression of basal dendrites requires cue(s) from the extracellular environment in the dentate gyrus. The apical dendrite designation is determined from among multiple growing dendrites of young developing neurons.
wn.com/Plos One Differentiation Of Apical And Basal Dendrites In Pyramidal Cells And Granule Cells...
Differentiation of Apical and Basal Dendrites in Pyramidal Cells and Granule Cells in Dissociated Hippocampal Cultures. You Kure Wu et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118482
Hippocampal pyramidal cells and dentate granule cells develop morphologically distinct dendritic arbors, yet also share some common features. Both cell types form a long apical dendrite which extends from the apex of the cell soma, while short basal dendrites are developed only in pyramidal cells. Using quantitative morphometric analyses of mouse hippocampal cultures, we evaluated the differences in dendritic arborization patterns between pyramidal and granule cells. Furthermore, we observed and described the final apical dendrite determination during dendritic polarization by time-lapse imaging. Pyramidal and granule cells in culture exhibited similar dendritic patterns with a single principal dendrite and several minor dendrites so that the cell types were not readily distinguished by appearance. While basal dendrites in granule cells are normally degraded by adulthood in vivo, cultured granule cells retained their minor dendrites. Asymmetric growth of a single principal dendrite harboring the Golgi was observed in both cell types soon after the onset of dendritic growth. Time-lapse imaging revealed that up until the second week in culture, final principal dendrite designation was not stabilized, but was frequently replaced by other minor dendrites. Before dendritic polarity was stabilized, the Golgi moved dynamically within the soma and was repeatedly repositioned at newly emerging principal dendrites. Our results suggest that polarized growth of the apical dendrite is regulated by cell intrinsic programs, while regression of basal dendrites requires cue(s) from the extracellular environment in the dentate gyrus. The apical dendrite designation is determined from among multiple growing dendrites of young developing neurons.
- published: 24 Feb 2015
- views: 9
PLoS ONE : Posture Affects How Robots and Infants Map Words to Objects
Posture Affects How Robots and Infants Map Words to Objects. Anthony F. Morse et al (2015), PLoS ONE http://journals.plos.org/plosone/article?id=10.1371/journal...
Posture Affects How Robots and Infants Map Words to Objects. Anthony F. Morse et al (2015), PLoS ONE http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116012
For infants, the first problem in learning a word is to map the word to its referent; a second problem is to remember that mapping when the word and/or referent are again encountered. Recent infant studies suggest that spatial location plays a key role in how infants solve both problems. Here we provide a new theoretical model and new empirical evidence on how the body – and its momentary posture – may be central to these processes. The present study uses a name-object mapping task in which names are either encountered in the absence of their target (experiments 1–3, 6 & 7), or when their target is present but in a location previously associated with a foil (experiments 4, 5, 8 & 9). A humanoid robot model (experiments 1–5) is used to instantiate and test the hypothesis that body-centric spatial location, and thus the bodies’ momentary posture, is used to centrally bind the multimodal features of heard names and visual objects. The robot model is shown to replicate existing infant data and then to generate novel predictions, which are tested in new infant studies (experiments 6–9). Despite spatial location being task-irrelevant in this second set of experiments, infants use body-centric spatial contingency over temporal contingency to map the name to object. Both infants and the robot remember the name-object mapping even in new spatial locations. However, the robot model shows how this memory can emerge –not from separating bodily information from the word-object mapping as proposed in previous models of the role of space in word-object mapping – but through the body’s momentary disposition in space.
wn.com/Plos One Posture Affects How Robots And Infants Map Words To Objects
Posture Affects How Robots and Infants Map Words to Objects. Anthony F. Morse et al (2015), PLoS ONE http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116012
For infants, the first problem in learning a word is to map the word to its referent; a second problem is to remember that mapping when the word and/or referent are again encountered. Recent infant studies suggest that spatial location plays a key role in how infants solve both problems. Here we provide a new theoretical model and new empirical evidence on how the body – and its momentary posture – may be central to these processes. The present study uses a name-object mapping task in which names are either encountered in the absence of their target (experiments 1–3, 6 & 7), or when their target is present but in a location previously associated with a foil (experiments 4, 5, 8 & 9). A humanoid robot model (experiments 1–5) is used to instantiate and test the hypothesis that body-centric spatial location, and thus the bodies’ momentary posture, is used to centrally bind the multimodal features of heard names and visual objects. The robot model is shown to replicate existing infant data and then to generate novel predictions, which are tested in new infant studies (experiments 6–9). Despite spatial location being task-irrelevant in this second set of experiments, infants use body-centric spatial contingency over temporal contingency to map the name to object. Both infants and the robot remember the name-object mapping even in new spatial locations. However, the robot model shows how this memory can emerge –not from separating bodily information from the word-object mapping as proposed in previous models of the role of space in word-object mapping – but through the body’s momentary disposition in space.
- published: 18 Mar 2015
- views: 13
PLoS ONE : Rapid Screening of Gene Function by Systemic Delivery of Morpholino Oligonucleotides...
Rapid Screening of Gene Function by Systemic Delivery of Morpholino Oligonucleotides to Live Mouse Embryos. Kathryn S. McClelland et al (2015), PLoS ONE, http:/...
Rapid Screening of Gene Function by Systemic Delivery of Morpholino Oligonucleotides to Live Mouse Embryos. Kathryn S. McClelland et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0114932
Traditional gene targeting methods in mice are complex and time consuming, especially when conditional deletion methods are required. Here, we describe a novel technique for assessing gene function by injection of modified antisense morpholino oligonucleotides (MOs) into the heart of mid-gestation mouse embryos. After allowing MOs to circulate through the embryonic vasculature, target tissues were explanted, cultured and analysed for expression of key markers. We established proof-of-principle by partially phenocopying known gene knockout phenotypes in the fetal gonads (Stra8, Sox9) and pancreas (Sox9). We also generated a novel double knockdown of Gli1 and Gli2, revealing defects in Leydig cell differentiation in the fetal testis. Finally, we gained insight into the roles of Adamts19 and Ctrb1, genes of unknown function in sex determination and gonadal development. These studies reveal the utility of this method as a means of first-pass analysis of gene function during organogenesis before committing to detailed genetic analysis.
wn.com/Plos One Rapid Screening Of Gene Function By Systemic Delivery Of Morpholino Oligonucleotides...
Rapid Screening of Gene Function by Systemic Delivery of Morpholino Oligonucleotides to Live Mouse Embryos. Kathryn S. McClelland et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0114932
Traditional gene targeting methods in mice are complex and time consuming, especially when conditional deletion methods are required. Here, we describe a novel technique for assessing gene function by injection of modified antisense morpholino oligonucleotides (MOs) into the heart of mid-gestation mouse embryos. After allowing MOs to circulate through the embryonic vasculature, target tissues were explanted, cultured and analysed for expression of key markers. We established proof-of-principle by partially phenocopying known gene knockout phenotypes in the fetal gonads (Stra8, Sox9) and pancreas (Sox9). We also generated a novel double knockdown of Gli1 and Gli2, revealing defects in Leydig cell differentiation in the fetal testis. Finally, we gained insight into the roles of Adamts19 and Ctrb1, genes of unknown function in sex determination and gonadal development. These studies reveal the utility of this method as a means of first-pass analysis of gene function during organogenesis before committing to detailed genetic analysis.
- published: 28 Jan 2015
- views: 4
PLoS ONE : Structure, Dynamics, and Interaction of Mycobacterium tuberculosis (Mtb) DprE1...
Structure, Dynamics, and Interaction of Mycobacterium tuberculosis (Mtb) DprE1 and DprE2 Examined by Molecular Modeling, Simulation, and Electrostatic Studies. ...
Structure, Dynamics, and Interaction of Mycobacterium tuberculosis (Mtb) DprE1 and DprE2 Examined by Molecular Modeling, Simulation, and Electrostatic Studies. Isha Bhutani et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0119771
The enzymes decaprenylphosphoryl-β-D-ribose oxidase (DprE1) and decaprenylphosphoryl-β-D-ribose-2-epimerase(DprE2) catalyze epimerization of decaprenylphosporyl ribose (DPR) todecaprenylphosporyl arabinose (DPA) and are critical for the survival of Mtb. Crystal structures of DprE1 so far reported display significant disordered regions and no structural information is known for DprE2. We used homology modeling, protein threading, molecular docking and dynamics studies to investigate the structural and dynamic features of Mtb DprE1 and DprE2 and DprE1-DprE2 complex. A three-dimensional model for DprE2 was generated using the threading approach coupled with ab initio modeling. A 50 ns simulation of DprE1 and DprE2 revealed the overall stability of the structures. Principal Component Analysis (PCA) demonstrated the convergence of sampling in both DprE1 and DprE2. In DprE1, residues in the 269–330 area showed considerable fluctuation in agreement with the regions of disorder observed in the reported crystal structures. In DprE2, large fluctuations were detected in residues 95–113, 146–157, and 197–226. The study combined docking and MD simulation studies to map and characterize the key residues involved in DprE1-DprE2 interaction. A 60 ns MD simulation for DprE1-DprE2 complex was also performed. Analysis of data revealed that the docked complex is stabilized by H-bonding, hydrophobic and ionic interactions. The key residues of DprE1 involved in DprE1-DprE2 interactions belong to the disordered region. We also examined the docked complex of DprE1-BTZ043 to investigate the binding pocket of DprE1 and its interactions with the inhibitor BTZ043. In summary, we hypothesize that DprE1-DprE2 interaction is crucial for the synthesis of DPA and DprE1-DprE2 complex may be a new therapeutic target amenable to pharmacological validation. The findings have important implications in tuberculosis (TB) drug discovery and will facilitate drug development efforts against TB.
wn.com/Plos One Structure, Dynamics, And Interaction Of Mycobacterium Tuberculosis (Mtb) Dpre1...
Structure, Dynamics, and Interaction of Mycobacterium tuberculosis (Mtb) DprE1 and DprE2 Examined by Molecular Modeling, Simulation, and Electrostatic Studies. Isha Bhutani et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0119771
The enzymes decaprenylphosphoryl-β-D-ribose oxidase (DprE1) and decaprenylphosphoryl-β-D-ribose-2-epimerase(DprE2) catalyze epimerization of decaprenylphosporyl ribose (DPR) todecaprenylphosporyl arabinose (DPA) and are critical for the survival of Mtb. Crystal structures of DprE1 so far reported display significant disordered regions and no structural information is known for DprE2. We used homology modeling, protein threading, molecular docking and dynamics studies to investigate the structural and dynamic features of Mtb DprE1 and DprE2 and DprE1-DprE2 complex. A three-dimensional model for DprE2 was generated using the threading approach coupled with ab initio modeling. A 50 ns simulation of DprE1 and DprE2 revealed the overall stability of the structures. Principal Component Analysis (PCA) demonstrated the convergence of sampling in both DprE1 and DprE2. In DprE1, residues in the 269–330 area showed considerable fluctuation in agreement with the regions of disorder observed in the reported crystal structures. In DprE2, large fluctuations were detected in residues 95–113, 146–157, and 197–226. The study combined docking and MD simulation studies to map and characterize the key residues involved in DprE1-DprE2 interaction. A 60 ns MD simulation for DprE1-DprE2 complex was also performed. Analysis of data revealed that the docked complex is stabilized by H-bonding, hydrophobic and ionic interactions. The key residues of DprE1 involved in DprE1-DprE2 interactions belong to the disordered region. We also examined the docked complex of DprE1-BTZ043 to investigate the binding pocket of DprE1 and its interactions with the inhibitor BTZ043. In summary, we hypothesize that DprE1-DprE2 interaction is crucial for the synthesis of DPA and DprE1-DprE2 complex may be a new therapeutic target amenable to pharmacological validation. The findings have important implications in tuberculosis (TB) drug discovery and will facilitate drug development efforts against TB.
- published: 19 Mar 2015
- views: 10
PLoS ONE : Pain Inhibition by Optogenetic Activation of Specific Anterior Cingulate Cortical Neurons
Pain Inhibition by Optogenetic Activation of Specific Anterior Cingulate Cortical Neurons. Ling Gu et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone...
Pain Inhibition by Optogenetic Activation of Specific Anterior Cingulate Cortical Neurons. Ling Gu et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0117746
Cumulative evidence from both humans and animals suggests that the anterior cingulate cortex (ACC) is important for pain-related perception, and thus a likely target for pain relief therapy. However, use of existing electrode based ACC stimulation has not significantly reduced pain, at least in part due to the lack of specificity and likely co-activation of both excitatory and inhibitory neurons. Herein, we report a dramatic reduction of pain behavior in transgenic mice by optogenetic stimulation of the inhibitory neural circuitry of the ACC expressing channelrhodopsin-2. Electrophysiological measurements confirmed that stimulation of ACC inhibitory neurons is associated with decreased neural activity in the ACC. Further, a distinct optogenetic stimulation intensity and frequency-dependent inhibition of spiking activity in the ACC was observed. Moreover, we confirmed specific electrophysiological responses from different neuronal units in the thalamus, in response to particular types of painful stimuli (i,e., formalin injection, pinch), which we found to be modulated by optogenetic control of the ACC inhibitory neurons. These results underscore the inhibition of the ACC as a clinical alternative in inhibiting chronic pain, and leads to a better understanding of the pain processing circuitry of the cingulate cortex.
wn.com/Plos One Pain Inhibition By Optogenetic Activation Of Specific Anterior Cingulate Cortical Neurons
Pain Inhibition by Optogenetic Activation of Specific Anterior Cingulate Cortical Neurons. Ling Gu et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0117746
Cumulative evidence from both humans and animals suggests that the anterior cingulate cortex (ACC) is important for pain-related perception, and thus a likely target for pain relief therapy. However, use of existing electrode based ACC stimulation has not significantly reduced pain, at least in part due to the lack of specificity and likely co-activation of both excitatory and inhibitory neurons. Herein, we report a dramatic reduction of pain behavior in transgenic mice by optogenetic stimulation of the inhibitory neural circuitry of the ACC expressing channelrhodopsin-2. Electrophysiological measurements confirmed that stimulation of ACC inhibitory neurons is associated with decreased neural activity in the ACC. Further, a distinct optogenetic stimulation intensity and frequency-dependent inhibition of spiking activity in the ACC was observed. Moreover, we confirmed specific electrophysiological responses from different neuronal units in the thalamus, in response to particular types of painful stimuli (i,e., formalin injection, pinch), which we found to be modulated by optogenetic control of the ACC inhibitory neurons. These results underscore the inhibition of the ACC as a clinical alternative in inhibiting chronic pain, and leads to a better understanding of the pain processing circuitry of the cingulate cortex.
- published: 25 Feb 2015
- views: 2
PLoS ONE : Drinking Songs: Alcohol Effects on Learned Song of Zebra Finches
KeSimpulan | Drinking Songs: Alcohol Effects on Learned Song of Zebra Finches. Christopher R. Olson et al. (2014), PLoS ONE http://dx.doi.org/10.1371/journal.po...
KeSimpulan | Drinking Songs: Alcohol Effects on Learned Song of Zebra Finches. Christopher R. Olson et al. (2014), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0115427
wn.com/Plos One Drinking Songs Alcohol Effects On Learned Song Of Zebra Finches
KeSimpulan | Drinking Songs: Alcohol Effects on Learned Song of Zebra Finches. Christopher R. Olson et al. (2014), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0115427
- published: 25 Dec 2014
- views: 5
PLoS ONE: Multiple Requirements of PLK1 during Mouse Oocyte Maturation
Multiple Requirements of PLK1 during Mouse Oocyte Maturation. Petr Solc et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0116783
Polo-like kinase ...
Multiple Requirements of PLK1 during Mouse Oocyte Maturation. Petr Solc et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0116783
Polo-like kinase 1 (PLK1) orchestrates multiple events of cell division. Although PLK1 function has been intensively studied in centriole-containing and rapidly cycling somatic cells, much less is known about its function in the meiotic divisions of mammalian oocytes, which arrest for a long period of time in prophase before meiotic resumption and lack centrioles for spindle assembly. Here, using specific small molecule inhibition combined with live mouse oocyte imaging, we comprehensively characterize meiotic PLK1’s functions. We show that PLK1 becomes activated at meiotic resumption on microtubule organizing centers (MTOCs) and later at kinetochores. PLK1 is required for efficient meiotic resumption by promoting nuclear envelope breakdown. PLK1 is also needed to recruit centrosomal proteins to acentriolar MTOCs to promote normal spindle formation, as well as for stable kinetochore-microtubule attachment. Consequently, PLK1 inhibition leads to metaphase I arrest with misaligned chromosomes activating the spindle assembly checkpoint (SAC). Unlike in mitosis, the metaphase I arrest is not bypassed by the inactivation of the SAC. We show that PLK1 is required for the full activation of the anaphase promoting complex/cyclosome (APC/C) by promoting the degradation of the APC/C inhibitor EMI1 and is therefore essential for entry into anaphase I. Moreover, our data suggest that PLK1 is required for proper chromosome segregation and the maintenance of chromosome condensation during the meiosis I-II transition, independently of the APC/C. Thus, our results define the meiotic roles of PLK1 in oocytes and reveal interesting differential requirements of PLK1 between mitosis and oocyte meiosis in mammals.
wn.com/Plos One Multiple Requirements Of Plk1 During Mouse Oocyte Maturation
Multiple Requirements of PLK1 during Mouse Oocyte Maturation. Petr Solc et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0116783
Polo-like kinase 1 (PLK1) orchestrates multiple events of cell division. Although PLK1 function has been intensively studied in centriole-containing and rapidly cycling somatic cells, much less is known about its function in the meiotic divisions of mammalian oocytes, which arrest for a long period of time in prophase before meiotic resumption and lack centrioles for spindle assembly. Here, using specific small molecule inhibition combined with live mouse oocyte imaging, we comprehensively characterize meiotic PLK1’s functions. We show that PLK1 becomes activated at meiotic resumption on microtubule organizing centers (MTOCs) and later at kinetochores. PLK1 is required for efficient meiotic resumption by promoting nuclear envelope breakdown. PLK1 is also needed to recruit centrosomal proteins to acentriolar MTOCs to promote normal spindle formation, as well as for stable kinetochore-microtubule attachment. Consequently, PLK1 inhibition leads to metaphase I arrest with misaligned chromosomes activating the spindle assembly checkpoint (SAC). Unlike in mitosis, the metaphase I arrest is not bypassed by the inactivation of the SAC. We show that PLK1 is required for the full activation of the anaphase promoting complex/cyclosome (APC/C) by promoting the degradation of the APC/C inhibitor EMI1 and is therefore essential for entry into anaphase I. Moreover, our data suggest that PLK1 is required for proper chromosome segregation and the maintenance of chromosome condensation during the meiosis I-II transition, independently of the APC/C. Thus, our results define the meiotic roles of PLK1 in oocytes and reveal interesting differential requirements of PLK1 between mitosis and oocyte meiosis in mammals.
- published: 08 Feb 2015
- views: 3
Plos One: Skorpion smider halen - og anus - under flugtforsøg
Video fra Plos One viser, hvordan visse skorpioner er i stand til at smide hale og anus for at flygte. Skorpionerne helede hurtigt og levede op til otte måneder...
Video fra Plos One viser, hvordan visse skorpioner er i stand til at smide hale og anus for at flygte. Skorpionerne helede hurtigt og levede op til otte måneder efter i fangenskab.
wn.com/Plos One Skorpion Smider Halen Og Anus Under Flugtforsøg
Video fra Plos One viser, hvordan visse skorpioner er i stand til at smide hale og anus for at flygte. Skorpionerne helede hurtigt og levede op til otte måneder efter i fangenskab.
- published: 04 Feb 2015
- views: 6
PLoS ONE : In Vivo Imaging and Characterization of Actin Microridges
In Vivo Imaging and Characterization of Actin Microridges. Pui-ying Lam et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0115639
Actin microridge...
In Vivo Imaging and Characterization of Actin Microridges. Pui-ying Lam et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0115639
Actin microridges form labyrinth like patterns on superficial epithelial cells across animal species. This highly organized assembly has been implicated in mucus retention and in the mechanical structure of mucosal surfaces, however the mechanisms that regulate actin microridges remain largely unknown. Here we characterize the composition and dynamics of actin microridges on the surface of zebrafish larvae using live imaging. Microridges contain phospho-tyrosine, cortactin and VASP, but not focal adhesion kinase. Time-lapse imaging reveals dynamic changes in the length and branching of microridges in intact animals. Transient perturbation of the microridge pattern occurs before cell division with rapid re-assembly during and after cytokinesis. Microridge assembly is maintained with constitutive activation of Rho or inhibition of myosin II activity. However, expression of dominant negative RhoA or Rac alters microridge organization, with an increase in distance between microridges. Latrunculin A treatment and photoconversion experiments suggest that the F-actin filaments are actively treadmilling in microridges. Accordingly, inhibition of Arp2/3 or PI3K signaling impairs microridge structure and length. Taken together, actin microridges in zebrafish represent a tractable in vivo model to probe pattern formation and dissect Arp2/3-mediated actin dynamics in vivo.
wn.com/Plos One In Vivo Imaging And Characterization Of Actin Microridges
In Vivo Imaging and Characterization of Actin Microridges. Pui-ying Lam et al (2015), PLoS ONE, http://dx.doi.org/10.1371/journal.pone.0115639
Actin microridges form labyrinth like patterns on superficial epithelial cells across animal species. This highly organized assembly has been implicated in mucus retention and in the mechanical structure of mucosal surfaces, however the mechanisms that regulate actin microridges remain largely unknown. Here we characterize the composition and dynamics of actin microridges on the surface of zebrafish larvae using live imaging. Microridges contain phospho-tyrosine, cortactin and VASP, but not focal adhesion kinase. Time-lapse imaging reveals dynamic changes in the length and branching of microridges in intact animals. Transient perturbation of the microridge pattern occurs before cell division with rapid re-assembly during and after cytokinesis. Microridge assembly is maintained with constitutive activation of Rho or inhibition of myosin II activity. However, expression of dominant negative RhoA or Rac alters microridge organization, with an increase in distance between microridges. Latrunculin A treatment and photoconversion experiments suggest that the F-actin filaments are actively treadmilling in microridges. Accordingly, inhibition of Arp2/3 or PI3K signaling impairs microridge structure and length. Taken together, actin microridges in zebrafish represent a tractable in vivo model to probe pattern formation and dissect Arp2/3-mediated actin dynamics in vivo.
- published: 29 Jan 2015
- views: 1
PLoS ONE : Function and Evolutionary Origin of Unicellular Camera-Type Eye Structure
Function and Evolutionary Origin of Unicellular Camera-Type Eye Structure. Shiho Hayakawa et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118415
...
Function and Evolutionary Origin of Unicellular Camera-Type Eye Structure. Shiho Hayakawa et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118415
The ocelloid is an extraordinary eyespot organelle found only in the dinoflagellate family Warnowiaceae. It contains retina- and lens-like structures called the retinal body and the hyalosome. The ocelloid has been an evolutionary enigma because of its remarkable resemblance to the multicellular camera-type eye. To determine if the ocelloid is functionally photoreceptive, we investigated the warnowiid dinoflagellate Erythropsidinium. Here, we show that the morphology of the retinal body changed depending on different illumination conditions and the hyalosome manifests the refractile nature. Identifying a rhodopsin gene fragment in Erythropsidinium ESTs that is expressed in the retinal body by in situ hybridization, we also show that ocelloids are actually light sensitive photoreceptors. The rhodopsin gene identified is most closely related to bacterial rhodopsins. Taken together, we suggest that the ocelloid is an intracellular camera-type eye, which might be originated from endosymbiotic origin.
wn.com/Plos One Function And Evolutionary Origin Of Unicellular Camera Type Eye Structure
Function and Evolutionary Origin of Unicellular Camera-Type Eye Structure. Shiho Hayakawa et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118415
The ocelloid is an extraordinary eyespot organelle found only in the dinoflagellate family Warnowiaceae. It contains retina- and lens-like structures called the retinal body and the hyalosome. The ocelloid has been an evolutionary enigma because of its remarkable resemblance to the multicellular camera-type eye. To determine if the ocelloid is functionally photoreceptive, we investigated the warnowiid dinoflagellate Erythropsidinium. Here, we show that the morphology of the retinal body changed depending on different illumination conditions and the hyalosome manifests the refractile nature. Identifying a rhodopsin gene fragment in Erythropsidinium ESTs that is expressed in the retinal body by in situ hybridization, we also show that ocelloids are actually light sensitive photoreceptors. The rhodopsin gene identified is most closely related to bacterial rhodopsins. Taken together, we suggest that the ocelloid is an intracellular camera-type eye, which might be originated from endosymbiotic origin.
- published: 03 Mar 2015
- views: 5
Plos One y la dilatación de las pupilas.
Maricuajis: la semana pasada tuvimos problemas para subir el vídeo, pero ya estamos aquí otra vez!!! Bechotos!!! ^oo^. Más ......
Maricuajis: la semana pasada tuvimos problemas para subir el vídeo, pero ya estamos aquí otra vez!!! Bechotos!!! ^oo^. Más ...
wn.com/Plos One Y La Dilatación De Las Pupilas.
Maricuajis: la semana pasada tuvimos problemas para subir el vídeo, pero ya estamos aquí otra vez!!! Bechotos!!! ^oo^. Más ...
PLoS ONE : How Do “Mute” Cicadas Produce Their Calling Songs?
How Do “Mute” Cicadas Produce Their Calling Songs? Changqing Luo et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118554
Insects have evolved a v...
How Do “Mute” Cicadas Produce Their Calling Songs? Changqing Luo et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118554
Insects have evolved a variety of structures and mechanisms to produce sounds, which are used for communication both within and between species. Among acoustic insects, cicada males are particularly known for their loud and diverse sounds which function importantly in communication. The main method of sound production in cicadas is the tymbal mechanism, and a relative small number of cicada species possess both tymbal and stridulatory organs. However, cicadas of the genus Karenia do not have any specialized sound-producing structures, so they are referred to as “mute”. This denomination is quite misleading, as they indeed produce sounds. Here, we investigate the sound-producing mechanism and acoustic communication of the “mute” cicada, Karenia caelatata, and discover a new sound-production mechanism for cicadas: i.e., K. caelatata produces impact sounds by banging the forewing costa against the operculum. The temporal, frequency and amplitude characteristics of the impact sounds are described. Morphological studies and reflectance-based analyses reveal that the structures involved in sound production of K. caelatata (i.e., forewing, operculum, cruciform elevation, and wing-holding groove on scutellum) are all morphologically modified. Acoustic playback experiments and behavioral observations suggest that the impact sounds of K. caelatata are used in intraspecific communication and function as calling songs. The new sound-production mechanism expands our knowledge on the diversity of acoustic signaling behavior in cicadas and further underscores the need for more bioacoustic studies on cicadas which lack tymbal mechanism.
wn.com/Plos One How Do “Mute” Cicadas Produce Their Calling Songs
How Do “Mute” Cicadas Produce Their Calling Songs? Changqing Luo et al (2015), PLoS ONE http://dx.doi.org/10.1371/journal.pone.0118554
Insects have evolved a variety of structures and mechanisms to produce sounds, which are used for communication both within and between species. Among acoustic insects, cicada males are particularly known for their loud and diverse sounds which function importantly in communication. The main method of sound production in cicadas is the tymbal mechanism, and a relative small number of cicada species possess both tymbal and stridulatory organs. However, cicadas of the genus Karenia do not have any specialized sound-producing structures, so they are referred to as “mute”. This denomination is quite misleading, as they indeed produce sounds. Here, we investigate the sound-producing mechanism and acoustic communication of the “mute” cicada, Karenia caelatata, and discover a new sound-production mechanism for cicadas: i.e., K. caelatata produces impact sounds by banging the forewing costa against the operculum. The temporal, frequency and amplitude characteristics of the impact sounds are described. Morphological studies and reflectance-based analyses reveal that the structures involved in sound production of K. caelatata (i.e., forewing, operculum, cruciform elevation, and wing-holding groove on scutellum) are all morphologically modified. Acoustic playback experiments and behavioral observations suggest that the impact sounds of K. caelatata are used in intraspecific communication and function as calling songs. The new sound-production mechanism expands our knowledge on the diversity of acoustic signaling behavior in cicadas and further underscores the need for more bioacoustic studies on cicadas which lack tymbal mechanism.
- published: 25 Feb 2015
- views: 2