• Take a spoon full 1 to 2 times a day (spoon included with the ProGSH95).


• Add 10 g (measuring scoop included) of ProGSH95 in 6 - 10oz (170 - 300 ml) of cold water or your favorite non-carbonated drink. You can even mix ProGSH95 with your yogurt.


• Mix in a plastic shaker cup. Do not use metal utensils or metal shaker to avoid denaturing the protein.


• Drink a full glass of water 5 minutes after taking your ProGSH95.


• ProGSH95 is soluble and has a pleasant taste.

• Strenghtens the immune system.


• Reduces stress.


• Helps with anti-aging.


• Improves energy and an overall sense of well being.


• Helps promote deep sleep.


• Helps improve mental acuity.


• Helps reduce inflammatory conditions.


• Helps improve the proper functioning of vital organs like the heart, lungs, liver and kidneys.


• Helps promote fitness and the level of workout performance.


• Helps to recover faster from intense workouts.

• Helps detoxify the body of some metals, chemicals, toxins and free radicals in general.


• It has the ability to detoxify from metals such as mercury, cadmium, iron, arsenic and lead.


• It also has the ability to detoxify from solvents, chemicals, xenobiotic substances, pesticides, herbicides, fungicides.


• Protects against the harmful effects from radiation treatments.

Medical Research

COMPETITION FOR GLUTATHIONE PRECURSORS BETWEEN THE IMMUNE SYSTEM AND THE SKELETAL MUSCLE: PATHOGENESIS OF CHRONIC FATIGUE SYNDROME

G. Bounous, J. Molson(2)
(1)Former Professor, Department of Surgery, McGill University, and career Investigation of the Medical Research Council of Canada
(2)1994 Quebec Cycling Champion. Road and Time Trial

MEDICAL HYPOTHESES (1999) 53(4): 347-349 - UF6D91999 HARCOURT PUBLISHERS LTD. - ARTICLE NO. MEHY. 1998.0780

SUMMARY - The chronic fatigue syndrome (CFS) is typically associated or follows a recognized or presumed infection. Abnormalities of both humoral and cellular immunity have been demonstrated in a substantial proportion of patients with CFS. The most consistent findings are of impaired lymphocyte responses to mitogen. As an antioxidant, glutathione (GSH) is essential for allowing the lymphocyte to express its full potential without being hampered by oxiradical accumulation. Hence, protracted challenge of the immunocytes may lead to cellular GSH depletion. Because GSH is also essential to aerobic muscular contraction, an undesirable competition for GSH precursors between the immune and muscular systems may develop. It is conceivable that the priority of the immune system for the survival of the host has drawn to this vital area the everdiminishing GSH precursors, thus depriving the skeletal muscle of adequate GSH precursors to sustain a normal aerobic metabolism resulting in fatigue and eventually myalgia. © 1999 Harcourt Publishers Ltd.

WHEY PROTEINS AS A FOOD SUPPLEMENT IN HIV-SEROPOSITIVE INDIVIDUALS

G. Bounous, S. Baruchel, J. Falutz, P. Gold
Departments of Surgery and Medicine, The Montreal General Hospital and McGill University, Montreal, Quebec

Invest Med, 16: 204-209, 1993

ABSTRACT – On the basis of numerous animal experiments, a pilot study was undertaken to evaluate the effect of undenatured, biologically active, dietary whey protein in 3 HIV-seropositive individuals over a period of 3 months. Whey protein concentrate was prepared so that the most thermosensitive proteins, such as serum albumin which contains 6 glutamylcysteine groups, would be in undenatured form. Whey protein powder dissolved in a drink of the patient’s choice was drunk cold in quantities that were increased progressively from 8.4 to 39.2 g per day. Patients took whey proteins without adverse side effects. In the 3 patients whose body weight had been stable in the preceding 2 months, weight gain increased progressively between 2 and 7 kg, with 2 of the patients reaching ideal body weight. Serum proteins, including albumin, remained unchanged and within normal range, indicating that protein replenishment per se was not likely the cause of increased body weight. The glutathione content of the blood mononuclear cells was, as expected, below normal values in all patients at the beginning of the study. Over the 3-month period, GSH levels increased and in one case rose by 70% to reach normal value. The increase in body weight observed in these patients did not correlate with increase in energy or protein intake. In conclusion, these preliminary data indicate that, in patients who maintain an adequate total caloric intake, the addition of “bioactive” whey protein concentrate as a significant portion of total protein intake increases body weight and shows elevation of glutathione (GSH) content of mononuclear cells toward normal levels. This pilot study will serve as a basis for a much larger clinical trial.

THE BIOLOGICAL ACTIVITY OF UNDENATURED DIETARY WHEY PROTEINS: ROLE OF GLUTATHIONE.

G. Bounous, P. Gold
Department of Surgery, Montreal General Hospital, Research Institute, Quebec

CLIN INVEST MED, 14: 296-309, 1991

ABSTRACT – This study compared the effects of different sources of whey protein concentrate (20 g/100 g diet) and of casein on the spleen, liver, and heart glutathione content of C3H/HeJ mice, and on the immune response of their spleen cells to sheep red blood cells. Body weight curves were similar in all dietary groups. Our data indicate that the humoral immune response is highest in mice fed a dietary whey protein concentrate exhibiting the highest solubility (undenatured conformation) and a greater relative concentration of the thermolabile cystine rich proteins. In addition, the mice fed this type of whey protein concentrate exhibit higher levels of tissue glutathione. The presence in the serum albumin fraction of glutamylcysteine groups (rare in food protein) and the specific intramolecular bond as related to the undenatured conformation of the molecule are considered to be key factors in the glutathione- promoting activity of the protein mixture.

WHEY PROTEINS IN CANCER PREVENTION

G. Bounous(1), G. Batist(2) and P. Gold(3)
(1)Professor of Surgery, McGill University, and Career Investigator of the Medical Research Council of Canada
(2)Director, Experimental Therapeutics, Department of Oncology, McGill University
(3)Chairman, Department of Medicine, McGill University, and Physician-in-Chief, The Montreal General Hospital

CANCER LETTERS, 57: 91-94, 1991

ABSTRACT – Epidemiological and experimental studies suggest that dietary milk products may exert an inhibitory effect on the development of several types of tumors. Some recent experiments in rodents indicate that the antitumor activity of the dairy products is in the protein fraction and more specifically in the whey protein component of milk. We and others have demonstrated that whey protein diets result in increased glutathione (GSH) concentration in a number of tissues, and that some of the beneficial effects of whey protein intake are abrogated by inhibition of GSH synthesis. Whey protein is particularly rich in substrates for GSH synthesis. We suggest that whey protein may be exerting its effect on carcinogenesis by enhancing GSH concentration.

DIETARY MILK PROTEINS INHIBIT THE DEVELOPMENT OF DIMETHYLHYDRAZINE-INDUCED MALIGNANCY

R. Papenburg(1), G. Bounous(1), D. Fleiszer(1), P. Gold(2)
Departments of (1) Surgery and (2) Medicine, The Montreal General Hospital and McGill University, Montreal, Quebec, Canada

TUMOR BIOL 11: 129-136, 1990

ABSTRACT – This study investigated the influence of two formula diets containing 20 g/100 g diet of either whey protein concentrate or casein, or Purina mouse chow on 1,2dimethylhydrazine (DMH)-induced colon carcinoma in A/J mice. Four weeks after the 24th DMH treatment the incidence of tumour and tumour area in the whey protein-fed mice was substantially less in comparison to either the casein or Purina groups. The Purina group exhibited the greatest tumour burden. At the end of the experiment all animals continuously fed the whey protein diet were found to be alive, whereas 33% of those on the casein or Purina diet had died. Animals fed Purina diet for 20 weeks and then switched to either milk protein diet for a further 8 weeks exhibited a decrease in tumour burden as compared to those animals fed the Purina diet continuously. Body weights were similar in all dietary groups. In conclusion, a whey protein diet appears to significantly influence the development of chemically induced colon tumours and the short-term survival of mice.

THE INFLUENCE OF DIETARY WHEY PROTEIN ON TISSUE GLUTATHIONE AND THE DISEASES OF AGING

Gustavo Bounous(1,2), Francine Gervais(1,3), Victor Amer(1,3), Gerald Batist(3), and Phil Gold(1,3)
The Montreal General Hospital Research Institute (1) and McGill University, Departments of Surgery (2), and Medicine (3)

CLIN INVEST MED, 12: 343-349, 1989

ABSTRACT – This study compared the effects of a whey-rich diet (20 g / 100 g diet), with that of Purina mouse chow or casein-rich diet (20 g / 100 g diet), on the liver and heart glutathione content and on the survival of old male C57BL / 6 NIA mice. The study was performed during a limited observation period of 6.3 months. In mice fed the whey protein-rich diet between 17 months and 20 months of age, the heart tissue and liver tissue glutathione content were enhanced above the corresponding values of the casein diet-fed and Purina-fed mice. Mice fed the whey protein diet at the onset of senescence, exhibited increased longevity as compared to mice fed Purina mouse chow over the 6.3 month observation period extending from the age of 21 months (corresponding to a human age of 55 years) to 26- 27 months of age (corresponding to a human age of 80 years), during which time 55% mortality was observed. The corresponding mean survival time of mice fed the defined casein diet is almost identical to that of Purina-fed controls. Body weight curves were similar in all three dietary groups. Hence, a whey protein diet appears to enhance the liver and heart glutathione concentration in aging mice and to increase longevity over a 6.3 month observation period.

IMMUNOENHANCING PROPERTY OF DIETARY WHEY PROTEIN IN MICE: ROLE OF GLUTATHIONE

G. Bounous, G. Batist, P. Gold
Montreal General Hospital, Quebec

CLIN INVEST MED, 12: 154-61, 1989

ABSTRACT – The spleen cells immune response to sheep red blood cells of C3H/HeJ mice fed a 20 g whey protein/100 g diet is substantially higher than that of mice fed an equivalent casein diet of similar nutritional efficiency. The present study indicates that the observed immunoenhancing effect of the whey protein mixture is dependent on the overall amino acid pattern resulting from the contribution of all its protein components. Whey protein contains substantially more cysteine than casein. Dietary cysteine is considered to be a rate limiting substrate for the synthesis of glutathione which is necessary for lymphocyte proliferation. Our studies show that enhancement of host humoral immune response is associated with greater and more sustained production of splenic glutathione during the antigen driven clonal expansion of the lymphocyte in whey protein fed mice in comparison to mice fed the equivalent casein or the cysteine-enriched casein diet. Hence the efficiency of dietary cysteine in inducing supernormal glutathione levels is greater when it is delivered in the whey protein than as free cysteine. Administration of S-(n-butyl) homocysteine sulfoximine, which reduces splenic glutathione level by half, produces a 4-5 fold drop in the humoral immune response of whey protein diet-fed mice. This is further evidence of the important role of glutathione in the immunoenhancing effect of dietary whey protein.

THE IMMUNOENHANCING PROPERTY OF DIETARY WHEY PROTEIN CONCENTRATE

Gustavo Bounous(1,2), Patricia A.L. Kongshavn(1,3) and Phil Gold(1,4)
(1) The Montreal General Hospital Research Institute
(2) Departments of Surgery, (3) Physiology, and (4) Medicine, McGill University, Montreal, Quebec
(ORIGINAL MANUSCRIPT SUBMITTED OCTOBER 22, 1987: ACCEPTED IN REVISED FORM JANUARY 25, 1988)

CLINICAL AND INVESTIGATIVE MEDICINE, VOL. 11, NO. 4, PP 271-278, 1988

ABSTRACT - The plaque-forming cell response to sheep red blood cells was found to be enhanced in mice fed a formula diet containing 20 g lactalbumin /100 g diet in comparison to mice fed equivalent formula diets of similar nutritional efficiency containing 20 g / 100 g diet of either casein, soy, wheat or corn protein, egg albumin, beef or fish protein, Spirulina maxima, or Scenedesmus protein, or Purina mouse chow. This effect was manifest after 2 weeks and persisted for at least 8 weeks of dietary treatment. Mixing lactalbumin with either casein or soy protein in a 20 g protein / 100 g diet formula significantly enhanced the immune response in comparison to that of mice fed diets containing 20% soy protein or casein. Key words: dietary whey protein, humoral immune response.

DIETARY WHEY PROTEIN INHIBITS THE DEVELOPMENT OF DIMETHYLHYDRAZINE-INDUCED MALIGNANCY

G. Bounous(1), R. Papenburg(1), P.A.L Kongshavn(2), P. Gold(3), and D. Fleiszer(1)
Departments of Surgery(1), Physiology(2), and Medicine(3), Montreal General Hospital and McGill University

CLIN INV MED, 11: 213-217, 1988

ABSTRACT – This study investigates the influence of two formula diets containing 20 g/100 g diet of either whey protein concentrate or casein or Purina mouse chow, on the humoral immune responsiveness and dimethylhydrazine induced colon carcinogenesis in A/J mice. After 20 weeks of dimethylhydrazine treatment, the number of plaque forming cells per spleen, following intravenous inoculation with 5 x 10 6 sheep red blood cells, was nearly three times greater in the whey protein-fed group than in the casein-fed mice although both values were substantially below normal. After 24 weeks of dimethylhydrazine treatment the incidence of tumors in the whey protein-fed mice was substantially lower than that in mice fed either the casein or Purina diet. Similarly, the tumor area was less in the whey protein group in comparison to either the casein or Purina groups, with some difference between casein and Purina groups. Body weight curves were similar in all dietary groups.
In conclusion, a whey protein diet appears to significantly inhibit the incidence and growth of chemically induced colon tumors in mice.

TREATMENT OF OBSTRUCTIVE AIRWAY DISEASE WITH A CYSTEINE DONOR PROTEIN SUPPLEMENT: A CASE REPORT

Bryce Lothian MD(1), Vijaylaxmi Grey PhD(1,2), R. John Kimoff MD(3), Larry Lands MD PhD(1,4)
(1)Department of Pediatrics, (2)Department of Biochemistry, (4)Division of Respiratory Medicine, McGill University Health Centre - Montreal Children’s Hospital, Montreal, Quebec, Canada
(3)Division of Respiratory Medicine, McGill University Health Centre-Royal Victoria Hospital, Montreal, Quebec,Canada

ACCEPTED FOR PUBLICATION IN “CHEST”

ABSTRACT: Oxidant/antioxidant imbalance can occur in obstructive airways disease, as a result of ongoing inflammation. Glutathione plays a major role in pulmonary antioxidant protection. As an alternative or complement to anti-inflammatory therapy, augmenting antioxidant protection could diminish the effects of inflammation. We describe a case of a patient with obstructive lung disease, responsive to corticosteroids, with low whole blood glutathione levels. Following one month of supplementation with a whey-based oral supplement, designed to provide glutathione precursors, whole blood glutathione levels and pulmonary function significantly and dramatically increased. The potential for such supplementation in pulmonary inflammatory conditions deserves further study.

WHEY PROTEIN CONCENTRATE (WPC) AND GLUTATHIONE MODULATION IN CANCER TREATMENT

Gustavo Bounous, M.D., F.R.C.S. (C)

Anticancer Research 20: 4785-4792, 2000

ABSTRACT - The glutathione (GSH) antioxidant system is foremost among the cellular protective mechanisms. Depletion of this small molecule is a common consequence of increased formation of reactive oxygen species during increased cellular activities. This phenomenon can occur in the lymphocytes during the development of the immune response and in the muscular cells during strenuous exercise. It is not surprising that so much research has been done, and is still being done on this small tripeptide molecule. Whey protein concentrate has been shown to represent an effective and safe cysteine donor for GSH replenishment during GSH depletion in immune deficiency states. Cysteine is the crucial limiting amino acid for intracellular GSH synthesis. Animal experiments showed that the concentrates of whey proteins also exhibit anti-carcinogenesis and anticancer activity. They do this via their effect on increasing GSH concentration in relevant tissues, and may have anti-tumor effect on low volume of tumor via stimulation of immunity through the GSH pathway. It is considered that oxygen radical generation is frequently a critical step in carcinogenesis, hence the effect of GSH on free radicals as well as carcinogen detoxification, could be important in inhibiting carcinogenesis induced by a number of different mechanisms. Case reports are resented which strongly suggest an anti-tumor effect of a whey protein dietary supplement in some urogenital cancers. This nontoxic dietary intervention, which is not based on the principles of current cancer chemotherapy, will hopefully attract the attention of laboratory and clinical oncologists.

MILK WHEY PROTEIN DECREASES OXYGEN FREE RADICAL PRODUCTION IN A MURINE MODEL OF CHRONIC IRON-OVERLOAD CARDIOMYOPATHY

WJ Bartfay, MT Davis, JM Medves, S Lugowski

CAN J CARDIOL VOL 19, NO 10, SEPTEMBER 2003

ABSTRACT – Chronic iron overload is a major cause of organ failure worldwide, but its pathogenesis remains to be elucidated. To examine in an experimental murine model of iron-overload cardiomyopathy the relation between milk whey protein and, first, the production of reactive oxygen free radical species and, second, antioxidant reserve status. B6D2F1 mice were randomly assigned to four treatment groups (n=8 per treatment group): placebo control; iron only; whey only; and iron with whey. Reactive oxygen free radical species in the heart were quantified by the cytotoxic aldehydes alondialdehyde (MDA), 4-hydroxynonenal (HNE) and hexanal, while antioxidant reserve status was quantified by glutathione (GSH) and glutathione peroxidase (GPx) activity in the heart tissue. Significantly decreased concentrations (pmol/100 mg wet weight tissue) of MDA (2468 ± 261), HNE (912 ± 38) and hexanal (5385 ± 927) were observed in the heart tissue of the group receiving iron with whey, in comparison with the iron-only treatment group (MDA 9307 ± 387, HNE 1416 ± 157, hexanal 14,874 ± 2955; P<0.001). Significantly increased GPx (141 ± 38 IU/L) and GSH (521 ± 136 IU/L) activity were observed in mice receiving iron with whey, in comparison with mice receiving iron only (GPx 100 ± 10 IU/L, GSH 446 ± 33 IU/L; P<0.001). Mice receiving iron treatments with whey supplementation had significantly lower concentrations of cytotoxic aldehydes and significantly higher cardiac levels of GPx and GSH activity than did iron-only treated mice. Additional basic research is warranted to examine the exact mechanisms by which milk whey protein protects the heart.