- published: 25 Nov 2016
- views: 190811
Lentivirus
Lentivirus (lente-, Latin for "slow") is a genus of viruses of the Retroviridae family, characterized by a long incubation period. Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells, so they are one of the most efficient methods of a gene delivery vector.HIV, SIV, FIV, EIAV, and Visna are all examples of lentiviruses.
Classification
Five serogroups of lentiviruses are recognized, reflecting the vertebrate hosts with which they are associated (primates, sheep and goats, horses, cats, and cattle). The primate lentiviruses are distinguished by the use of CD4 protein as a receptor and the absence of dUTPase. Some groups have cross-reactive gag antigens (e.g., the ovine, caprine and feline lentiviruses). Antibodies to gag antigens in lions and other large felids indicate the existence of other viruses related to FIV and the ovine/caprine lentiviruses.
Morphology
This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Lentivirus
Podcasts:
-
The Basics of the Recombinant Lentivirus System
How do recombinant lentivirus systems work? Lentiviruses are members of the Retroviridae family of viruses, with HIV-1 being the most widely studied. Lentiviral vectors are able to deliver genes into a broad range of mammalian cell lines and tissue types and to infect both dividing and non-dividing cells. Recombinant lentivirus systems and lentiviral transfection/transduction are powerful tools and methods for gene delivery and gene therapy. Because they can stably integrate transgenes into the host genome, lentiviruses are unique tools for generating stable cell lines for long term expression. In this video we will introduce you to the basic structure and genome map of the HIV Lentivirus and explain the differences and advantages of using first, second, and third generation recombinant le...
published: 25 Nov 2016 -
Lentivirus in Cell Engineering
Lentiviruses are one of the most commonly used viral vectors for gene delivery in cell engineering. By integrating its genetic payload into the host cell genome, lentiviruses ensure that the transgene is passed on to generations of cells, allowing for stable expression of the transgene over time. With broad tissue and cell tropism as well as an ability to infect dividing and non-dividing cells, lentiviruses have become the vector of choice for researchers when they need to create a stable cell line. GenScript provides researchers with the highly intact and functional quality lentivirus particles to ensure their next cell engineering project is a success! To order your high quality lentivirus packaging today, visit https://www.genscript.com/research-grade-lentiviral-vector-packaging.html. ...
published: 27 Apr 2021 -
The Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!
Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged? Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs. In this video, we'll outline the steps/protocol for packaging lentiviruses, including: • Considerations before you start [1:02] • Basic packaging protocol [01:33] • Storing your lentiviruses [4:19] • Infection test experiment [4:56] • Additional tips and tricks for lentivirus production After watching this video, you’ll be well on your way t...
published: 19 Jun 2020 -
Lentivirus Transduction Protocol: Infecting your target cells
So you've packaged and harvested your lentiviruses and you're ready to infect your target cells! But, what lentivirus transduction protocol should you use? Depending on whether you're infecting adherent or suspension cells or difficult-to-transduce cell types, we have different infection recommendations for you! In this video, we'll explain: → How to perform a lentiviral transduction experiment → An overview of the spinoculation and reverse transduction methods → How to confirm transgene expression Lentiviruses are extremely useful gene delivery systems for delivering cDNA, shRNA, and sgRNA to stably express, knockdown, or knockout your gene of interest in your target cells. Once you learn how to use lentiviruses for your own experiment, you'll be able to target not only dividing cells b...
published: 23 Jul 2021 -
Production of Lentivirus
This video describes a procedure which to package lentiviruses. Packaging refers to the preparation of competent virus from DNA vectors. Lentiviral vector production systems are based on a 'split' system, where the natural viral genome has been split into individual helper plasmid constructs. This splitting of the different viral elements into four separate vectors diminishes the risk of creating a replication-capable virus by adventitious recombination of the lentiviral genome. Here, a vector containing the shRNA of interest and three packaging vectors (p-VSVG, pRSV, pMDL) are transiently transfected into human 293 cells.
published: 17 Jul 2023 -
2nd vs 3rd Gen Lentivirus #shorts
published: 30 Jan 2023 -
Lentiviral Infection
( http://www.abnova.com ) - Lentivirus is a genus of slow viruses of the Retroviridae family that have long incubation periods and cause chronic, progressive, often fatal diseases affecting many organs in humans. This video shows you how to infect culture cells with lentivirus. It is an important step before the production of lentiviral vector. More videos at Abnova http://www.abnova.com
published: 01 Jul 2011 -
Lentivirus Titration: How to Determine Viral Titer
So, you've harvested your lentiviruses and are ready to infect your cells! Hold on a minute - do you know how many lentiviral particles you have? It's time to calculate your lentivirus titer! In this fourth video of our lentivirus series we'll show you various methods for determining viral titer. This video covers: • Functional vs. Physical Titer • How to determine functional titer: FACS, Colony Formation Assay, qPCR • How to determine physical titer: p24 ELISA, qRT-PCR • Which titering method you should use • How to calculate titer There are two different types of titer measurements: functional and physical titer. Functional titer measures how many viral particles are actually able to infect cells, whereas physical titer simply measures how many total viral particles there are, both fun...
published: 09 Feb 2021 -
Lentivirus VIH
published: 23 Jan 2020 -
Gene delivery tools webinar | Lentivirus
Learn all about lentivirus in this webinar from our experts
published: 08 Dec 2022
developed with YouTube
7:00
The Basics of the Recombinant Lentivirus System
- Order: Reorder
- Duration: 7:00
- Uploaded Date: 25 Nov 2016
- views: 190811
How do recombinant lentivirus systems work? Lentiviruses are members of the Retroviridae family of viruses, with HIV-1 being the most widely studied. Lentiviral...
How do recombinant lentivirus systems work? Lentiviruses are members of the Retroviridae family of viruses, with HIV-1 being the most widely studied. Lentiviral vectors are able to deliver genes into a broad range of mammalian cell lines and tissue types and to infect both dividing and non-dividing cells. Recombinant lentivirus systems and lentiviral transfection/transduction are powerful tools and methods for gene delivery and gene therapy. Because they can stably integrate transgenes into the host genome, lentiviruses are unique tools for generating stable cell lines for long term expression. In this video we will introduce you to the basic structure and genome map of the HIV Lentivirus and explain the differences and advantages of using first, second, and third generation recombinant lentivirus packaging systems. In the recombinant system, the HIV lentiviral genome is split into multiple plasmids (e.g. lentiviral transfer plasmids, packaging plasmids and envelop plasmids) that are delivered separately to ensure the introduced lentivirus cannot replicate in host cells. Thus, though natural lentiviruses such as HIV, SIV, FIV, EIAV, and Visna can cause chronic and even fatal diseases, scientists have managed to reduce the biosafety risks to a minimal level and convert this group of viruses into a tool for research and treatment of diseases such as cancers.
For more information on the recombinant lentivirus expression system and for a list of the sources used, please visit:
➜ Knowledge Base: https://info.abmgood.com/lentivirus-system-introduction
Check out our other video series:
➜ Cell Culture - An Introduction: https://youtu.be/RpDke-Sadzo
➜ CRISPR Cas9: https://youtu.be/1aJxXWkE3Ek?list=PLTt9kKfqE_0Ei8_rQsrfm01-zQtABTn0Z
➜ Next Generation Sequencing: https://youtu.be/jFCD8Q6qSTM?list=PLTt9kKfqE_0Gem8hIcJEn7YcesuuKdt_n
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
➜ Facebook: https://goo.gl/hc9KrG
➜ Twitter: https://goo.gl/gGGtT9
➜ LinkedIn: https://goo.gl/kSmbht
➜ Google+: https://goo.gl/5bRNwC
https://wn.com/The_Basics_Of_The_Recombinant_Lentivirus_System
How do recombinant lentivirus systems work? Lentiviruses are members of the Retroviridae family of viruses, with HIV-1 being the most widely studied. Lentiviral vectors are able to deliver genes into a broad range of mammalian cell lines and tissue types and to infect both dividing and non-dividing cells. Recombinant lentivirus systems and lentiviral transfection/transduction are powerful tools and methods for gene delivery and gene therapy. Because they can stably integrate transgenes into the host genome, lentiviruses are unique tools for generating stable cell lines for long term expression. In this video we will introduce you to the basic structure and genome map of the HIV Lentivirus and explain the differences and advantages of using first, second, and third generation recombinant lentivirus packaging systems. In the recombinant system, the HIV lentiviral genome is split into multiple plasmids (e.g. lentiviral transfer plasmids, packaging plasmids and envelop plasmids) that are delivered separately to ensure the introduced lentivirus cannot replicate in host cells. Thus, though natural lentiviruses such as HIV, SIV, FIV, EIAV, and Visna can cause chronic and even fatal diseases, scientists have managed to reduce the biosafety risks to a minimal level and convert this group of viruses into a tool for research and treatment of diseases such as cancers.
For more information on the recombinant lentivirus expression system and for a list of the sources used, please visit:
➜ Knowledge Base: https://info.abmgood.com/lentivirus-system-introduction
Check out our other video series:
➜ Cell Culture - An Introduction: https://youtu.be/RpDke-Sadzo
➜ CRISPR Cas9: https://youtu.be/1aJxXWkE3Ek?list=PLTt9kKfqE_0Ei8_rQsrfm01-zQtABTn0Z
➜ Next Generation Sequencing: https://youtu.be/jFCD8Q6qSTM?list=PLTt9kKfqE_0Gem8hIcJEn7YcesuuKdt_n
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
➜ Facebook: https://goo.gl/hc9KrG
➜ Twitter: https://goo.gl/gGGtT9
➜ LinkedIn: https://goo.gl/kSmbht
➜ Google+: https://goo.gl/5bRNwC
1:34
Lentivirus in Cell Engineering
- Order: Reorder
- Duration: 1:34
- Uploaded Date: 27 Apr 2021
- views: 24994
Lentiviruses are one of the most commonly used viral vectors for gene delivery in cell engineering. By integrating its genetic payload into the host cell genome...
Lentiviruses are one of the most commonly used viral vectors for gene delivery in cell engineering. By integrating its genetic payload into the host cell genome, lentiviruses ensure that the transgene is passed on to generations of cells, allowing for stable expression of the transgene over time. With broad tissue and cell tropism as well as an ability to infect dividing and non-dividing cells, lentiviruses have become the vector of choice for researchers when they need to create a stable cell line. GenScript provides researchers with the highly intact and functional quality lentivirus particles to ensure their next cell engineering project is a success!
To order your high quality lentivirus packaging today, visit https://www.genscript.com/research-grade-lentiviral-vector-packaging.html.
www.genscript.com
#WeMakeProteinandMore
https://wn.com/Lentivirus_In_Cell_Engineering
Lentiviruses are one of the most commonly used viral vectors for gene delivery in cell engineering. By integrating its genetic payload into the host cell genome, lentiviruses ensure that the transgene is passed on to generations of cells, allowing for stable expression of the transgene over time. With broad tissue and cell tropism as well as an ability to infect dividing and non-dividing cells, lentiviruses have become the vector of choice for researchers when they need to create a stable cell line. GenScript provides researchers with the highly intact and functional quality lentivirus particles to ensure their next cell engineering project is a success!
To order your high quality lentivirus packaging today, visit https://www.genscript.com/research-grade-lentiviral-vector-packaging.html.
www.genscript.com
#WeMakeProteinandMore
- published: 27 Apr 2021
- views: 24994
6:08
The Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!
- Order: Reorder
- Duration: 6:08
- Uploaded Date: 19 Jun 2020
- views: 66423
Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after ...
Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged?
Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.
In this video, we'll outline the steps/protocol for packaging lentiviruses, including:
• Considerations before you start [1:02]
• Basic packaging protocol [01:33]
• Storing your lentiviruses [4:19]
• Infection test experiment [4:56]
• Additional tips and tricks for lentivirus production
After watching this video, you’ll be well on your way to becoming a lentivirus production champion!
[4:05] Note: An error in pronunciation. It is a 0.45 µm (micron) filter (not micromolar) that is recommended for use.
You can read more about how to package lentiviruses and get a hard copy of the protocol here:
➜ https://info.abmgood.com/lentivirus-system-production-protocol
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
➜ Facebook: https://www.facebook.com/appliedbiological.materials/
➜ Twitter: https://twitter.com/abm_good
➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material
➜ Instagram: https://www.instagram.com/abm_good
Background music credit: "Back To Portland" by TrackTribe
https://wn.com/The_Basics_Of_Lentivirus_Production_Packaging_Protocol,_Tips,_And_More
Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged?
Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.
In this video, we'll outline the steps/protocol for packaging lentiviruses, including:
• Considerations before you start [1:02]
• Basic packaging protocol [01:33]
• Storing your lentiviruses [4:19]
• Infection test experiment [4:56]
• Additional tips and tricks for lentivirus production
After watching this video, you’ll be well on your way to becoming a lentivirus production champion!
[4:05] Note: An error in pronunciation. It is a 0.45 µm (micron) filter (not micromolar) that is recommended for use.
You can read more about how to package lentiviruses and get a hard copy of the protocol here:
➜ https://info.abmgood.com/lentivirus-system-production-protocol
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
➜ Facebook: https://www.facebook.com/appliedbiological.materials/
➜ Twitter: https://twitter.com/abm_good
➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material
➜ Instagram: https://www.instagram.com/abm_good
Background music credit: "Back To Portland" by TrackTribe
- published: 19 Jun 2020
- views: 66423
9:36
Lentivirus Transduction Protocol: Infecting your target cells
- Order: Reorder
- Duration: 9:36
- Uploaded Date: 23 Jul 2021
- views: 42254
So you've packaged and harvested your lentiviruses and you're ready to infect your target cells! But, what lentivirus transduction protocol should you use? Depe...
So you've packaged and harvested your lentiviruses and you're ready to infect your target cells! But, what lentivirus transduction protocol should you use? Depending on whether you're infecting adherent or suspension cells or difficult-to-transduce cell types, we have different infection recommendations for you! In this video, we'll explain:
→ How to perform a lentiviral transduction experiment
→ An overview of the spinoculation and reverse transduction methods
→ How to confirm transgene expression
Lentiviruses are extremely useful gene delivery systems for delivering cDNA, shRNA, and sgRNA to stably express, knockdown, or knockout your gene of interest in your target cells. Once you learn how to use lentiviruses for your own experiment, you'll be able to target not only dividing cells but also post-mitotic, non-dividing cells like neurons.
→ For more information about lentiviruses, access our free learning resources: https://info.abmgood.com/lentivirus-system
→ Access our full protocol for lentivirus transduction:
https://www.abmgood.com/lentiviral-system-documents
Request a free sample of our ViralEntry™ Transduction Enhancer:
→ https://www.abmgood.com/Transduction-Enhancers.html
Watch our video "Multiplicity of Infection (MOI): What is it and how do I calculate it?":
→ https://www.youtube.com/watch?v=rfw_rcP5XOA
Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences:
➜ Facebook: https://www.facebook.com/appliedbiological.materials/
➜ Twitter: https://twitter.com/abm_good
➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material
➜ Instagram: https://www.instagram.com/abm_good
➜ Blog: https://info.abmgood.com/blog
https://wn.com/Lentivirus_Transduction_Protocol_Infecting_Your_Target_Cells
So you've packaged and harvested your lentiviruses and you're ready to infect your target cells! But, what lentivirus transduction protocol should you use? Depending on whether you're infecting adherent or suspension cells or difficult-to-transduce cell types, we have different infection recommendations for you! In this video, we'll explain:
→ How to perform a lentiviral transduction experiment
→ An overview of the spinoculation and reverse transduction methods
→ How to confirm transgene expression
Lentiviruses are extremely useful gene delivery systems for delivering cDNA, shRNA, and sgRNA to stably express, knockdown, or knockout your gene of interest in your target cells. Once you learn how to use lentiviruses for your own experiment, you'll be able to target not only dividing cells but also post-mitotic, non-dividing cells like neurons.
→ For more information about lentiviruses, access our free learning resources: https://info.abmgood.com/lentivirus-system
→ Access our full protocol for lentivirus transduction:
https://www.abmgood.com/lentiviral-system-documents
Request a free sample of our ViralEntry™ Transduction Enhancer:
→ https://www.abmgood.com/Transduction-Enhancers.html
Watch our video "Multiplicity of Infection (MOI): What is it and how do I calculate it?":
→ https://www.youtube.com/watch?v=rfw_rcP5XOA
Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences:
➜ Facebook: https://www.facebook.com/appliedbiological.materials/
➜ Twitter: https://twitter.com/abm_good
➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material
➜ Instagram: https://www.instagram.com/abm_good
➜ Blog: https://info.abmgood.com/blog
- published: 23 Jul 2021
- views: 42254
11:43
Production of Lentivirus
- Order: Reorder
- Duration: 11:43
- Uploaded Date: 17 Jul 2023
- views: 565
This video describes a procedure which to package lentiviruses. Packaging refers to the preparation of competent virus from DNA vectors. Lentiviral vector produ...
This video describes a procedure which to package lentiviruses. Packaging refers to the preparation of competent virus from DNA vectors. Lentiviral vector production systems are based on a 'split' system, where the natural viral genome has been split into individual helper plasmid constructs. This splitting of the different viral elements into four separate vectors diminishes the risk of creating a replication-capable virus by adventitious recombination of the lentiviral genome. Here, a vector containing the shRNA of interest and three packaging vectors (p-VSVG, pRSV, pMDL) are transiently transfected into human 293 cells.
https://wn.com/Production_Of_Lentivirus
This video describes a procedure which to package lentiviruses. Packaging refers to the preparation of competent virus from DNA vectors. Lentiviral vector production systems are based on a 'split' system, where the natural viral genome has been split into individual helper plasmid constructs. This splitting of the different viral elements into four separate vectors diminishes the risk of creating a replication-capable virus by adventitious recombination of the lentiviral genome. Here, a vector containing the shRNA of interest and three packaging vectors (p-VSVG, pRSV, pMDL) are transiently transfected into human 293 cells.
- published: 17 Jul 2023
- views: 565
0:59
2nd vs 3rd Gen Lentivirus #shorts
- Order: Reorder
- Duration: 0:59
- Uploaded Date: 30 Jan 2023
- views: 1183
- published: 30 Jan 2023
- views: 1183
2:42
Lentiviral Infection
- Order: Reorder
- Duration: 2:42
- Uploaded Date: 01 Jul 2011
- views: 20622
( http://www.abnova.com ) - Lentivirus is a genus of slow viruses of the Retroviridae family that have long incubation periods and cause chronic, progressive, o...
( http://www.abnova.com ) - Lentivirus is a genus of slow viruses of the Retroviridae family that have long incubation periods and cause chronic, progressive, often fatal diseases affecting many organs in humans. This video shows you how to infect culture cells with lentivirus. It is an important step before the production of lentiviral vector. More videos at Abnova http://www.abnova.com
https://wn.com/Lentiviral_Infection
( http://www.abnova.com ) - Lentivirus is a genus of slow viruses of the Retroviridae family that have long incubation periods and cause chronic, progressive, often fatal diseases affecting many organs in humans. This video shows you how to infect culture cells with lentivirus. It is an important step before the production of lentiviral vector. More videos at Abnova http://www.abnova.com
- published: 01 Jul 2011
- views: 20622
9:16
Lentivirus Titration: How to Determine Viral Titer
- Order: Reorder
- Duration: 9:16
- Uploaded Date: 09 Feb 2021
- views: 31011
So, you've harvested your lentiviruses and are ready to infect your cells! Hold on a minute - do you know how many lentiviral particles you have? It's time to c...
So, you've harvested your lentiviruses and are ready to infect your cells! Hold on a minute - do you know how many lentiviral particles you have? It's time to calculate your lentivirus titer!
In this fourth video of our lentivirus series we'll show you various methods for determining viral titer. This video covers:
• Functional vs. Physical Titer
• How to determine functional titer: FACS, Colony Formation Assay, qPCR
• How to determine physical titer: p24 ELISA, qRT-PCR
• Which titering method you should use
• How to calculate titer
There are two different types of titer measurements: functional and physical titer. Functional titer measures how many viral particles are actually able to infect cells, whereas physical titer simply measures how many total viral particles there are, both functional and non-functional.
Calculating viral titer is a foundational skill for every cell biology lab and we hope our video helps you plan your own viral titering experiment with confidence!
For more learning resources on lentiviruses, visit:
➜ https://info.abmgood.com/lentivirus-system
Get a FREE sample of our ViralEntry™ Transduction Enhancer:
➜ https://www.abmgood.com/Transduction-Enhancers.html
Learn more about our abmVacations+ Rewards Program🎁 :
➜ https://www.abmgood.com/rewards-program.html
Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences:
➜ Facebook: https://www.facebook.com/appliedbiological.materials/
➜ Twitter: https://twitter.com/abm_good
➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material
➜ Instagram: https://www.instagram.com/abm_good
➜ Blog: https://info.abmgood.com/blog
https://wn.com/Lentivirus_Titration_How_To_Determine_Viral_Titer
So, you've harvested your lentiviruses and are ready to infect your cells! Hold on a minute - do you know how many lentiviral particles you have? It's time to calculate your lentivirus titer!
In this fourth video of our lentivirus series we'll show you various methods for determining viral titer. This video covers:
• Functional vs. Physical Titer
• How to determine functional titer: FACS, Colony Formation Assay, qPCR
• How to determine physical titer: p24 ELISA, qRT-PCR
• Which titering method you should use
• How to calculate titer
There are two different types of titer measurements: functional and physical titer. Functional titer measures how many viral particles are actually able to infect cells, whereas physical titer simply measures how many total viral particles there are, both functional and non-functional.
Calculating viral titer is a foundational skill for every cell biology lab and we hope our video helps you plan your own viral titering experiment with confidence!
For more learning resources on lentiviruses, visit:
➜ https://info.abmgood.com/lentivirus-system
Get a FREE sample of our ViralEntry™ Transduction Enhancer:
➜ https://www.abmgood.com/Transduction-Enhancers.html
Learn more about our abmVacations+ Rewards Program🎁 :
➜ https://www.abmgood.com/rewards-program.html
Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences:
➜ Facebook: https://www.facebook.com/appliedbiological.materials/
➜ Twitter: https://twitter.com/abm_good
➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material
➜ Instagram: https://www.instagram.com/abm_good
➜ Blog: https://info.abmgood.com/blog
- published: 09 Feb 2021
- views: 31011
34:46
Lentivirus VIH
- Order: Reorder
- Duration: 34:46
- Uploaded Date: 23 Jan 2020
- views: 40653
- published: 23 Jan 2020
- views: 40653
35:45
Gene delivery tools webinar | Lentivirus
- Order: Reorder
- Duration: 35:45
- Uploaded Date: 08 Dec 2022
- views: 1187
Learn all about lentivirus in this webinar from our experts
Learn all about lentivirus in this webinar from our experts
https://wn.com/Gene_Delivery_Tools_Webinar_|_Lentivirus
Learn all about lentivirus in this webinar from our experts
- published: 08 Dec 2022
- views: 1187
7:00
The Basics of the Recombinant Lentivirus System
How do recombinant lentivirus systems work? Lentiviruses are members of the Retroviridae f...
published: 25 Nov 2016
The Basics of the Recombinant Lentivirus System
The Basics of the Recombinant Lentivirus System
- Report rights infringement
- published: 25 Nov 2016
- views: 190811
How do recombinant lentivirus systems work? Lentiviruses are members of the Retroviridae family of viruses, with HIV-1 being the most widely studied. Lentiviral vectors are able to deliver genes into a broad range of mammalian cell lines and tissue types and to infect both dividing and non-dividing cells. Recombinant lentivirus systems and lentiviral transfection/transduction are powerful tools and methods for gene delivery and gene therapy. Because they can stably integrate transgenes into the host genome, lentiviruses are unique tools for generating stable cell lines for long term expression. In this video we will introduce you to the basic structure and genome map of the HIV Lentivirus and explain the differences and advantages of using first, second, and third generation recombinant lentivirus packaging systems. In the recombinant system, the HIV lentiviral genome is split into multiple plasmids (e.g. lentiviral transfer plasmids, packaging plasmids and envelop plasmids) that are delivered separately to ensure the introduced lentivirus cannot replicate in host cells. Thus, though natural lentiviruses such as HIV, SIV, FIV, EIAV, and Visna can cause chronic and even fatal diseases, scientists have managed to reduce the biosafety risks to a minimal level and convert this group of viruses into a tool for research and treatment of diseases such as cancers.
For more information on the recombinant lentivirus expression system and for a list of the sources used, please visit:
➜ Knowledge Base: https://info.abmgood.com/lentivirus-system-introduction
Check out our other video series:
➜ Cell Culture - An Introduction: https://youtu.be/RpDke-Sadzo
➜ CRISPR Cas9: https://youtu.be/1aJxXWkE3Ek?list=PLTt9kKfqE_0Ei8_rQsrfm01-zQtABTn0Z
➜ Next Generation Sequencing: https://youtu.be/jFCD8Q6qSTM?list=PLTt9kKfqE_0Gem8hIcJEn7YcesuuKdt_n
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
➜ Facebook: https://goo.gl/hc9KrG
➜ Twitter: https://goo.gl/gGGtT9
➜ LinkedIn: https://goo.gl/kSmbht
➜ Google+: https://goo.gl/5bRNwC
1:34
Lentivirus in Cell Engineering
Lentiviruses are one of the most commonly used viral vectors for gene delivery in cell eng...
published: 27 Apr 2021
Lentivirus in Cell Engineering
Lentivirus in Cell Engineering
- Report rights infringement
- published: 27 Apr 2021
- views: 24994
Lentiviruses are one of the most commonly used viral vectors for gene delivery in cell engineering. By integrating its genetic payload into the host cell genome, lentiviruses ensure that the transgene is passed on to generations of cells, allowing for stable expression of the transgene over time. With broad tissue and cell tropism as well as an ability to infect dividing and non-dividing cells, lentiviruses have become the vector of choice for researchers when they need to create a stable cell line. GenScript provides researchers with the highly intact and functional quality lentivirus particles to ensure their next cell engineering project is a success!
To order your high quality lentivirus packaging today, visit https://www.genscript.com/research-grade-lentiviral-vector-packaging.html.
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6:08
The Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!
Want to package recombinant lentiviruses? What packaging cell line should you use? How do ...
published: 19 Jun 2020
The Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!
The Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!
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- published: 19 Jun 2020
- views: 66423
Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged?
Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.
In this video, we'll outline the steps/protocol for packaging lentiviruses, including:
• Considerations before you start [1:02]
• Basic packaging protocol [01:33]
• Storing your lentiviruses [4:19]
• Infection test experiment [4:56]
• Additional tips and tricks for lentivirus production
After watching this video, you’ll be well on your way to becoming a lentivirus production champion!
[4:05] Note: An error in pronunciation. It is a 0.45 µm (micron) filter (not micromolar) that is recommended for use.
You can read more about how to package lentiviruses and get a hard copy of the protocol here:
➜ https://info.abmgood.com/lentivirus-system-production-protocol
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Background music credit: "Back To Portland" by TrackTribe
9:36
Lentivirus Transduction Protocol: Infecting your target cells
So you've packaged and harvested your lentiviruses and you're ready to infect your target ...
published: 23 Jul 2021
Lentivirus Transduction Protocol: Infecting your target cells
Lentivirus Transduction Protocol: Infecting your target cells
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- published: 23 Jul 2021
- views: 42254
So you've packaged and harvested your lentiviruses and you're ready to infect your target cells! But, what lentivirus transduction protocol should you use? Depending on whether you're infecting adherent or suspension cells or difficult-to-transduce cell types, we have different infection recommendations for you! In this video, we'll explain:
→ How to perform a lentiviral transduction experiment
→ An overview of the spinoculation and reverse transduction methods
→ How to confirm transgene expression
Lentiviruses are extremely useful gene delivery systems for delivering cDNA, shRNA, and sgRNA to stably express, knockdown, or knockout your gene of interest in your target cells. Once you learn how to use lentiviruses for your own experiment, you'll be able to target not only dividing cells but also post-mitotic, non-dividing cells like neurons.
→ For more information about lentiviruses, access our free learning resources: https://info.abmgood.com/lentivirus-system
→ Access our full protocol for lentivirus transduction:
https://www.abmgood.com/lentiviral-system-documents
Request a free sample of our ViralEntry™ Transduction Enhancer:
→ https://www.abmgood.com/Transduction-Enhancers.html
Watch our video "Multiplicity of Infection (MOI): What is it and how do I calculate it?":
→ https://www.youtube.com/watch?v=rfw_rcP5XOA
Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences:
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11:43
Production of Lentivirus
This video describes a procedure which to package lentiviruses. Packaging refers to the pr...
published: 17 Jul 2023
Production of Lentivirus
Production of Lentivirus
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- published: 17 Jul 2023
- views: 565
This video describes a procedure which to package lentiviruses. Packaging refers to the preparation of competent virus from DNA vectors. Lentiviral vector production systems are based on a 'split' system, where the natural viral genome has been split into individual helper plasmid constructs. This splitting of the different viral elements into four separate vectors diminishes the risk of creating a replication-capable virus by adventitious recombination of the lentiviral genome. Here, a vector containing the shRNA of interest and three packaging vectors (p-VSVG, pRSV, pMDL) are transiently transfected into human 293 cells.
0:59
2nd vs 3rd Gen Lentivirus #shorts
published: 30 Jan 2023
2nd vs 3rd Gen Lentivirus #shorts
2nd vs 3rd Gen Lentivirus #shorts
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- published: 30 Jan 2023
- views: 1183
2:42
Lentiviral Infection
( http://www.abnova.com ) - Lentivirus is a genus of slow viruses of the Retroviridae fami...
published: 01 Jul 2011
Lentiviral Infection
Lentiviral Infection
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- published: 01 Jul 2011
- views: 20622
( http://www.abnova.com ) - Lentivirus is a genus of slow viruses of the Retroviridae family that have long incubation periods and cause chronic, progressive, often fatal diseases affecting many organs in humans. This video shows you how to infect culture cells with lentivirus. It is an important step before the production of lentiviral vector. More videos at Abnova http://www.abnova.com
9:16
Lentivirus Titration: How to Determine Viral Titer
So, you've harvested your lentiviruses and are ready to infect your cells! Hold on a minut...
published: 09 Feb 2021
Lentivirus Titration: How to Determine Viral Titer
Lentivirus Titration: How to Determine Viral Titer
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- published: 09 Feb 2021
- views: 31011
So, you've harvested your lentiviruses and are ready to infect your cells! Hold on a minute - do you know how many lentiviral particles you have? It's time to calculate your lentivirus titer!
In this fourth video of our lentivirus series we'll show you various methods for determining viral titer. This video covers:
• Functional vs. Physical Titer
• How to determine functional titer: FACS, Colony Formation Assay, qPCR
• How to determine physical titer: p24 ELISA, qRT-PCR
• Which titering method you should use
• How to calculate titer
There are two different types of titer measurements: functional and physical titer. Functional titer measures how many viral particles are actually able to infect cells, whereas physical titer simply measures how many total viral particles there are, both functional and non-functional.
Calculating viral titer is a foundational skill for every cell biology lab and we hope our video helps you plan your own viral titering experiment with confidence!
For more learning resources on lentiviruses, visit:
➜ https://info.abmgood.com/lentivirus-system
Get a FREE sample of our ViralEntry™ Transduction Enhancer:
➜ https://www.abmgood.com/Transduction-Enhancers.html
Learn more about our abmVacations+ Rewards Program🎁 :
➜ https://www.abmgood.com/rewards-program.html
Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences:
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35:45
Gene delivery tools webinar | Lentivirus
Learn all about lentivirus in this webinar from our experts
published: 08 Dec 2022
Gene delivery tools webinar | Lentivirus
Gene delivery tools webinar | Lentivirus
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- published: 08 Dec 2022
- views: 1187
Learn all about lentivirus in this webinar from our experts
Lentivirus
Lentivirus (lente-, Latin for "slow") is a genus of viruses of the Retroviridae family, characterized by a long incubation period. Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells, so they are one of the most efficient methods of a gene delivery vector.HIV, SIV, FIV, EIAV, and Visna are all examples of lentiviruses.
Classification
Five serogroups of lentiviruses are recognized, reflecting the vertebrate hosts with which they are associated (primates, sheep and goats, horses, cats, and cattle). The primate lentiviruses are distinguished by the use of CD4 protein as a receptor and the absence of dUTPase. Some groups have cross-reactive gag antigens (e.g., the ovine, caprine and feline lentiviruses). Antibodies to gag antigens in lions and other large felids indicate the existence of other viruses related to FIV and the ovine/caprine lentiviruses.
Morphology
This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Lentivirus
The Basics of the Recombinant Lentivirus System...
Lentivirus in Cell Engineering...
The Basics of Lentivirus Production/Packaging: Pro...
Lentivirus Transduction Protocol: Infecting your t...
Production of Lentivirus...
2nd vs 3rd Gen Lentivirus #shorts...
Lentiviral Infection...
Lentivirus Titration: How to Determine Viral Titer...
Lentivirus VIH...
Gene delivery tools webinar | Lentivirus...
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The findings were published in the journal Blood. [...]. This is an abstract of the document ... Attachments Original Link Permalink. Disclaimer ... (noodl. 118635320) .
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Researchers at Children's Hospital of Philadelphia Find Lentiviral Gene Transfer Improves Human Alpha Globin Production for the Treatment of Alpha Thalassemia
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Houston Forward Times
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FDA Approves Groundbreaking Cell-Based Gene Therapies for Sickle Cell Disease
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13 Dec 2023
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Vector Biomed Selected As Preferred Lentiviral Vector Manufacturing Partner For California Institute For Regenerative Medicine
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