Natalie du Toit (pronounced /dʉˈtɔɪ/; born 29 January 1984) is a South African swimmer. She is best known for the gold medals she won at the 2004 Paralympic Games as well as the Commonwealth Games. She was one of two Paralympians to compete at the 2008 Summer Olympics in Beijing; the other being table tennis player Natalia Partyka. Du Toit became the first amputee ever to qualify for the Olympics, where she placed 16th in the 10K, "Marathon", swim.

Du Toit was born in Cape Town, South Africa and attended Wynberg Girls' High School. She began competing internationally in swimming at the age of 14. In February 2001 her left leg was amputated at the knee after she was hit by a car while riding her scooter back to school after swimming practice. Three months later, before she had started walking again, she was back in the pool with the intention of competing in the 2002 Commonwealth Games. Du Toit swims without the aid of a prosthetic limb.

She completed her scholastic education at the Reddam House in Cape Town after which she studied for a Bachelor of Science degree at the University of Cape Town, specializing in genetics and physiology. In her free time she does motivational speaking.