Labatt Brewing Company Ltd. (French: Brasseries Labatt du Canada Ltée) is a Canadian beer company founded by John Kinder Labatt in 1847 in London, Ontario. In 1995, it was purchased by Belgian brewer Interbrew; it is now part of Anheuser-Busch InBev. Labatt is the largest brewer in Canada.

In the United States, Labatt brand beers are sold under license by Labatt USA, which since 2009 has been fully independent of the Canadian firm and a subsidiary of the privately-held North American Breweries of Rochester, New York.

In 1915, Prohibition began in Canada when public bars were banned in Saskatchewan. A year later prohibition was instituted in Ontario as well, affecting all 64 breweries in the province. Although some provinces totally banned alcohol manufacture, some permitted production for export to the United States. Labatt survived by producing full strength beer for export south of the border and by introducing two "temperance ales" with less than two per cent alcohol for sale in Ontario. However, the Canadian beer industry suffered a second blow when Prohibition began in the U.S. in 1919. When Prohibition was repealed in Ontario in 1926, just 15 breweries remained and only Labatt retained its original management. This resulted in a strengthened industry position. In 1945 Labatt became a publicly traded company with the issuance of 900,000 shares.