- published: 04 Nov 2011
- views: 288735
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Check out our free eLearning resources at ncbionetwork.org/educational-resources/elearning.
This short animation demonstrates the inner workings of a spectrophotometer. Practice using a virtual spectrophotometer: http://ncbionetwork.org/educational-resources/interactive-elearning-tools/spectrophotometry
Watch the most exciting lab safety video ever! "Zombie College: The 5 Rules of Lab Safety" http://youtu.be/S6WARqVdWrE
Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches the exit slit. Then the light interacts with the sample. From this point, the detector measures the transmittance and absorbance of the sample. Transmittance refers to the amount of light that passes completely through the sample and strikes the detector. Absorbance is a measurement of light that is absorbed by the sample. The detector senses the light being transmitted through the sample and converts this information into a digital display.
The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it interacts with light.
In this video, we'll demonstrate how to use a spectrophotometer to perform a basic experiment: determining the absorbance spectrum of red food coloring.
Included in the video is a short animation that illustrates the inner workings of a spectrophotometer.
Special thanks to Joe Issa and the Gaston College Biotechnology department for helping to make this video.
Practice using a virtual spec: http://www.ncbionetwork.org/educational-resources/elearning
- published: 07 Nov 2011
- views: 157705
Spectrophotometry, transmittance, absorbance and the Beer-Lambert Law. Created by Sal Khan.
Watch the next lesson: https://www.khanacademy.org/science/chemistry/chem-kinetics/spectrophotometry-tutorial/v/spectrophotometry-example?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry
Missed the previous lesson? https://www.khanacademy.org/science/chemistry/chem-kinetics/arrhenius-equation/v/catalysts?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry
Chemistry on Khan Academy: Did you know that everything is made out of chemicals? Chemistry is the study of matter: its composition, properties, and reactivity. This material roughly covers a first-year high school or college course, and a good understanding of algebra is helpful.
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s Chemistry channel: https://www.youtube.com/channel/UCyEot66LrwWFEMONvrIBh3A?sub_confirmation=1
Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
- published: 15 Oct 2010
- views: 285040
This video goes over the calibration and use of the Genesys 20 spectrophotometer used in the general chemistry lab.
- published: 04 Sep 2014
- views: 21497
http://www.fireflysci.com/calibration-kits/photometric-linearity-potassium-dichromate-kit
Learn how to calibrate a UV VIS spectrophotometer using potassium dichromate calibration filters.
I’m going to turn on our spectrophotometer and let it heat up for 45 minutes.
Let’s go ahead and choose a wavelength to calibrate. For potassium dichromate there are four different wavelengths you can choose. I’m going to set our machine to test 235nm.
Now we have to insert the perchloric acid blank that came with our potassium dichromate kit. When we insert the blank we’re going to get a reading, so we have to blank the machine now with the blank still in the cuvette holder.
When the machine is reading zero we can insert one of the potassium dichromate filters. Let’s go with the 20 mg/L filter. Now we’ll check the reading against the certificate of calibration.
Let’s go ahead and test 40 mg/L at 235 nm.
When you switch to different wavelengths, you have to insert the blank and blank the machine.
The reason we use a blank is because we only want to measure the absorbance of the potassium dichromate and not the cuvette or the perchloric acid. The blank accounts for the percholic acid and the cuvette so when we zero the machine we are eliminating these factors and leaving us only with the potassium dichromate.
Remember to check your certificate of calibration for the tolerance of your potassium dichromate kit. Also check your spectrophotometers manual to find the photometric accuracy tolerance of your machine and add this to the tolerance on the certificate of calibration.
Thanks for taking the time to watch this video. Please give us a thumbs up and leave us your questions and comments below.
If you need potassium dichromate filters you can visit our website at fireflysci.com and look for our calibration kits.
See ya next time!
- published: 21 May 2015
- views: 16224
Spectrophotometry
In chemistry, spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light, near-ultraviolet, and near-infrared, but does not cover time-resolved spectroscopic techniques.
Spectrophotometry uses photometers that can measure a light beam's intensity as a function of its color (wavelength) known as spectrophotometers. Important features of spectrophotometers are spectral bandwidth, (the range of colors it can transmit through the test sample), and the percentage of sample-transmission, and the logarithmic range of sample-absorption and sometimes a percentage of reflectance measurement.
A spectrophotometer is commonly used for the measurement of transmittance or reflectance of solutions, transparent or opaque solids, such as polished glass, or gases. However they can also be designed to measure the diffusivity on any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. Within these ranges of light, calibrations are needed on the machine using standards that vary in type depending on the wavelength of the photometric determination.
This page contains text from Wikipedia, the Free Encyclopedia -
https://wn.com/Spectrophotometry
This article is licensed under the Creative Commons Attribution-ShareAlike 3.0 Unported License, which means that you can copy and modify it as long as the entire work (including additions) remains under this license.
This article is licensed under the Creative Commons Attribution-ShareAlike 3.0 Unported License, which means that you can copy and modify it as long as the entire work (including additions) remains under this license.
Khan Academy
Khan Academy is a non-profit educational organization created in 2006 by educator Salman Khan with the aim of providing a free, world-class education for anyone, anywhere. The organization produces short lectures in the form of YouTube videos. In addition to micro lectures, the organization's website features practice exercises and tools for educators. All resources are available for free to anyone around the world. The main language of the website is English, but the content is also available in other languages.
History
The founder of the organization, Salman Khan, was born in New Orleans, Louisiana, United States to immigrant parents from Bangladesh and India. After earning three degrees from the Massachusetts Institute of Technology (a BS in mathematics, a BS in electrical engineering and computer science, and an MEng in electrical engineering and computer science), he pursued an MBA from Harvard Business School.
In late 2004, Khan began tutoring his cousin Nadia who needed help with math using Yahoo!'s Doodle notepad.When other relatives and friends sought similar help, he decided that it would be more practical to distribute the tutorials on YouTube. The videos' popularity and the testimonials of appreciative students prompted Khan to quit his job in finance as a hedge fund analyst at Connective Capital Management in 2009, and focus on the tutorials (then released under the moniker "Khan Academy") full-time.
This page contains text from Wikipedia, the Free Encyclopedia -
https://wn.com/Khan_Academy
This article is licensed under the Creative Commons Attribution-ShareAlike 3.0 Unported License, which means that you can copy and modify it as long as the entire work (including additions) remains under this license.
This article is licensed under the Creative Commons Attribution-ShareAlike 3.0 Unported License, which means that you can copy and modify it as long as the entire work (including additions) remains under this license.
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0:59How does a spectrophotometer work?
How does a spectrophotometer work?How does a spectrophotometer work?
Check out our free eLearning resources at ncbionetwork.org/educational-resources/elearning. This short animation demonstrates the inner workings of a spectrophotometer. Practice using a virtual spectrophotometer: http://ncbionetwork.org/educational-resources/interactive-elearning-tools/spectrophotometry Watch the most exciting lab safety video ever! "Zombie College: The 5 Rules of Lab Safety" http://youtu.be/S6WARqVdWrE Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches the exit slit. Then the light interacts with the sample. From this point, the detector measures the transmittance and absorbance of the sample. Transmittance refers to the amount of light that passes completely through the sample and strikes the detector. Absorbance is a measurement of light that is absorbed by the sample. The detector senses the light being transmitted through the sample and converts this information into a digital display. -
5:44How To Use A Spectrophotometer
How To Use A SpectrophotometerHow To Use A Spectrophotometer
-
6:27The Spectrophotometer: A demo and practice experiment
The Spectrophotometer: A demo and practice experimentThe Spectrophotometer: A demo and practice experiment
The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it interacts with light. In this video, we'll demonstrate how to use a spectrophotometer to perform a basic experiment: determining the absorbance spectrum of red food coloring. Included in the video is a short animation that illustrates the inner workings of a spectrophotometer. Special thanks to Joe Issa and the Gaston College Biotechnology department for helping to make this video. Practice using a virtual spec: http://www.ncbionetwork.org/educational-resources/elearning -
13:06Spectrophotometry introduction | Kinetics | Chemistry | Khan Academy
Spectrophotometry introduction | Kinetics | Chemistry | Khan AcademySpectrophotometry introduction | Kinetics | Chemistry | Khan Academy
Spectrophotometry, transmittance, absorbance and the Beer-Lambert Law. Created by Sal Khan. Watch the next lesson: https://www.khanacademy.org/science/chemistry/chem-kinetics/spectrophotometry-tutorial/v/spectrophotometry-example?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry Missed the previous lesson? https://www.khanacademy.org/science/chemistry/chem-kinetics/arrhenius-equation/v/catalysts?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry Chemistry on Khan Academy: Did you know that everything is made out of chemicals? Chemistry is the study of matter: its composition, properties, and reactivity. This material roughly covers a first-year high school or college course, and a good understanding of algebra is helpful. About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content. For free. For everyone. Forever. #YouCanLearnAnything Subscribe to Khan Academy’s Chemistry channel: https://www.youtube.com/channel/UCyEot66LrwWFEMONvrIBh3A?sub_confirmation=1 Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy -
6:48How a Simple UV-visible Spectrophotometer Works
How a Simple UV-visible Spectrophotometer Works -
3:42TRU Chemistry Labs: How To Calibrate and use the Spectrophotometer
TRU Chemistry Labs: How To Calibrate and use the SpectrophotometerTRU Chemistry Labs: How To Calibrate and use the Spectrophotometer
This video goes over the calibration and use of the Genesys 20 spectrophotometer used in the general chemistry lab. -
2:55How to use a Spectrophotometer
How to use a SpectrophotometerHow to use a Spectrophotometer
-
2:36How to Calibrate a Spectrophotometer with Potassium dichromate
How to Calibrate a Spectrophotometer with Potassium dichromateHow to Calibrate a Spectrophotometer with Potassium dichromate
http://www.fireflysci.com/calibration-kits/photometric-linearity-potassium-dichromate-kit Learn how to calibrate a UV VIS spectrophotometer using potassium dichromate calibration filters. I’m going to turn on our spectrophotometer and let it heat up for 45 minutes. Let’s go ahead and choose a wavelength to calibrate. For potassium dichromate there are four different wavelengths you can choose. I’m going to set our machine to test 235nm. Now we have to insert the perchloric acid blank that came with our potassium dichromate kit. When we insert the blank we’re going to get a reading, so we have to blank the machine now with the blank still in the cuvette holder. When the machine is reading zero we can insert one of the potassium dichromate filters. Let’s go with the 20 mg/L filter. Now we’ll check the reading against the certificate of calibration. Let’s go ahead and test 40 mg/L at 235 nm. When you switch to different wavelengths, you have to insert the blank and blank the machine. The reason we use a blank is because we only want to measure the absorbance of the potassium dichromate and not the cuvette or the perchloric acid. The blank accounts for the percholic acid and the cuvette so when we zero the machine we are eliminating these factors and leaving us only with the potassium dichromate. Remember to check your certificate of calibration for the tolerance of your potassium dichromate kit. Also check your spectrophotometers manual to find the photometric accuracy tolerance of your machine and add this to the tolerance on the certificate of calibration. Thanks for taking the time to watch this video. Please give us a thumbs up and leave us your questions and comments below. If you need potassium dichromate filters you can visit our website at fireflysci.com and look for our calibration kits. See ya next time! -
2:50Spectrophotometer Use
Spectrophotometer UseSpectrophotometer Use
-
1:32Spectrophotometer
SpectrophotometerSpectrophotometer
How to use the spectrophotometer correctly
-
How does a spectrophotometer work?
Check out our free eLearning resources at ncbionetwork.org/educational-resources/elearning. This short animation demonstrates the inner workings of a spectrophotometer. Practice using a virtual spectrophotometer: http://ncbionetwork.org/educational-resources/interactive-elearning-tools/spectrophotometry Watch the most exciting lab safety video ever! "Zombie College: The 5 Rules of Lab Safety" http://youtu.be/S6WARqVdWrE Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches the exit slit. Then the light interacts with the sample. From this point, the detecto...
published: 04 Nov 2011 -
How To Use A Spectrophotometer
published: 04 Mar 2014 -
The Spectrophotometer: A demo and practice experiment
The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it interacts with light. In this video, we'll demonstrate how to use a spectrophotometer to perform a basic experiment: determining the absorbance spectrum of red food coloring. Included in the video is a short animation that illustrates the inner workings of a spectrophotometer. Special thanks to Joe Issa and the Gaston College Biotechnology department for helping to make this video. Practice using a virtual spec: http://www.ncbionetwork.org/educational-resources/elearning
published: 07 Nov 2011 -
Spectrophotometry introduction | Kinetics | Chemistry | Khan Academy
Spectrophotometry, transmittance, absorbance and the Beer-Lambert Law. Created by Sal Khan. Watch the next lesson: https://www.khanacademy.org/science/chemistry/chem-kinetics/spectrophotometry-tutorial/v/spectrophotometry-example?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry Missed the previous lesson? https://www.khanacademy.org/science/chemistry/chem-kinetics/arrhenius-equation/v/catalysts?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry Chemistry on Khan Academy: Did you know that everything is made out of chemicals? Chemistry is the study of matter: its composition, properties, and reactivity. This material roughly covers a first-year high school or college course, and a good understanding of algebra is helpful. About Khan Academy: Khan Academy offers practice exercises,...
published: 15 Oct 2010 -
-
TRU Chemistry Labs: How To Calibrate and use the Spectrophotometer
This video goes over the calibration and use of the Genesys 20 spectrophotometer used in the general chemistry lab.
published: 04 Sep 2014 -
How to use a Spectrophotometer
published: 16 Jan 2015 -
How to Calibrate a Spectrophotometer with Potassium dichromate
http://www.fireflysci.com/calibration-kits/photometric-linearity-potassium-dichromate-kit Learn how to calibrate a UV VIS spectrophotometer using potassium dichromate calibration filters. I’m going to turn on our spectrophotometer and let it heat up for 45 minutes. Let’s go ahead and choose a wavelength to calibrate. For potassium dichromate there are four different wavelengths you can choose. I’m going to set our machine to test 235nm. Now we have to insert the perchloric acid blank that came with our potassium dichromate kit. When we insert the blank we’re going to get a reading, so we have to blank the machine now with the blank still in the cuvette holder. When the machine is reading zero we can insert one of the potassium dichromate filters. Let’s go with the 20 mg/L filter...
published: 21 May 2015 -
Spectrophotometer Use
published: 13 Jun 2017 -
Spectrophotometer
How to use the spectrophotometer correctly
published: 24 Nov 2009
How does a spectrophotometer work?
- Order: Reorder
- Duration: 0:59
- Updated: 04 Nov 2011
- views: 288735
Check out our free eLearning resources at ncbionetwork.org/educational-resources/elearning.
This short animation demonstrates the inner workings of a spectroph...
Check out our free eLearning resources at ncbionetwork.org/educational-resources/elearning.
This short animation demonstrates the inner workings of a spectrophotometer. Practice using a virtual spectrophotometer: http://ncbionetwork.org/educational-resources/interactive-elearning-tools/spectrophotometry
Watch the most exciting lab safety video ever! "Zombie College: The 5 Rules of Lab Safety" http://youtu.be/S6WARqVdWrE
Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches the exit slit. Then the light interacts with the sample. From this point, the detector measures the transmittance and absorbance of the sample. Transmittance refers to the amount of light that passes completely through the sample and strikes the detector. Absorbance is a measurement of light that is absorbed by the sample. The detector senses the light being transmitted through the sample and converts this information into a digital display.
https://wn.com/How_Does_A_Spectrophotometer_Work
Check out our free eLearning resources at ncbionetwork.org/educational-resources/elearning.
This short animation demonstrates the inner workings of a spectrophotometer. Practice using a virtual spectrophotometer: http://ncbionetwork.org/educational-resources/interactive-elearning-tools/spectrophotometry
Watch the most exciting lab safety video ever! "Zombie College: The 5 Rules of Lab Safety" http://youtu.be/S6WARqVdWrE
Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches the exit slit. Then the light interacts with the sample. From this point, the detector measures the transmittance and absorbance of the sample. Transmittance refers to the amount of light that passes completely through the sample and strikes the detector. Absorbance is a measurement of light that is absorbed by the sample. The detector senses the light being transmitted through the sample and converts this information into a digital display.
- published: 04 Nov 2011
- views: 288735
How To Use A Spectrophotometer
- Order: Reorder
- Duration: 5:44
- Updated: 04 Mar 2014
- views: 70966
- published: 04 Mar 2014
- views: 70966
The Spectrophotometer: A demo and practice experiment
- Order: Reorder
- Duration: 6:27
- Updated: 07 Nov 2011
- views: 157705
The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it i...
The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it interacts with light.
In this video, we'll demonstrate how to use a spectrophotometer to perform a basic experiment: determining the absorbance spectrum of red food coloring.
Included in the video is a short animation that illustrates the inner workings of a spectrophotometer.
Special thanks to Joe Issa and the Gaston College Biotechnology department for helping to make this video.
Practice using a virtual spec: http://www.ncbionetwork.org/educational-resources/elearning
https://wn.com/The_Spectrophotometer_A_Demo_And_Practice_Experiment
The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it interacts with light.
In this video, we'll demonstrate how to use a spectrophotometer to perform a basic experiment: determining the absorbance spectrum of red food coloring.
Included in the video is a short animation that illustrates the inner workings of a spectrophotometer.
Special thanks to Joe Issa and the Gaston College Biotechnology department for helping to make this video.
Practice using a virtual spec: http://www.ncbionetwork.org/educational-resources/elearning
- published: 07 Nov 2011
- views: 157705
Spectrophotometry introduction | Kinetics | Chemistry | Khan Academy
- Order: Reorder
- Duration: 13:06
- Updated: 15 Oct 2010
- views: 285040
Spectrophotometry, transmittance, absorbance and the Beer-Lambert Law. Created by Sal Khan.
Watch the next lesson: https://www.khanacademy.org/science/chemistr...
Spectrophotometry, transmittance, absorbance and the Beer-Lambert Law. Created by Sal Khan.
Watch the next lesson: https://www.khanacademy.org/science/chemistry/chem-kinetics/spectrophotometry-tutorial/v/spectrophotometry-example?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry
Missed the previous lesson? https://www.khanacademy.org/science/chemistry/chem-kinetics/arrhenius-equation/v/catalysts?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry
Chemistry on Khan Academy: Did you know that everything is made out of chemicals? Chemistry is the study of matter: its composition, properties, and reactivity. This material roughly covers a first-year high school or college course, and a good understanding of algebra is helpful.
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s Chemistry channel: https://www.youtube.com/channel/UCyEot66LrwWFEMONvrIBh3A?sub_confirmation=1
Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
https://wn.com/Spectrophotometry_Introduction_|_Kinetics_|_Chemistry_|_Khan_Academy
Spectrophotometry, transmittance, absorbance and the Beer-Lambert Law. Created by Sal Khan.
Watch the next lesson: https://www.khanacademy.org/science/chemistry/chem-kinetics/spectrophotometry-tutorial/v/spectrophotometry-example?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry
Missed the previous lesson? https://www.khanacademy.org/science/chemistry/chem-kinetics/arrhenius-equation/v/catalysts?utm_source=YT&utm;_medium=Desc&utm;_campaign=chemistry
Chemistry on Khan Academy: Did you know that everything is made out of chemicals? Chemistry is the study of matter: its composition, properties, and reactivity. This material roughly covers a first-year high school or college course, and a good understanding of algebra is helpful.
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s Chemistry channel: https://www.youtube.com/channel/UCyEot66LrwWFEMONvrIBh3A?sub_confirmation=1
Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
- published: 15 Oct 2010
- views: 285040
How a Simple UV-visible Spectrophotometer Works
- Order: Reorder
- Duration: 6:48
- Updated: 29 Sep 2014
- views: 116275
Professor Davis describes a simple example of a double-beam UV-visible spectrophotometer and how it is used to determine the %transmittance of a sample, which c...
Professor Davis describes a simple example of a double-beam UV-visible spectrophotometer and how it is used to determine the %transmittance of a sample, which can be mathematically converted into absorbance for convenience.
https://wn.com/How_A_Simple_Uv_Visible_Spectrophotometer_Works
TRU Chemistry Labs: How To Calibrate and use the Spectrophotometer
- Order: Reorder
- Duration: 3:42
- Updated: 04 Sep 2014
- views: 21497
This video goes over the calibration and use of the Genesys 20 spectrophotometer used in the general chemistry lab.
This video goes over the calibration and use of the Genesys 20 spectrophotometer used in the general chemistry lab.
https://wn.com/Tru_Chemistry_Labs_How_To_Calibrate_And_Use_The_Spectrophotometer
This video goes over the calibration and use of the Genesys 20 spectrophotometer used in the general chemistry lab.
- published: 04 Sep 2014
- views: 21497
How to use a Spectrophotometer
- Order: Reorder
- Duration: 2:55
- Updated: 16 Jan 2015
- views: 7899
- published: 16 Jan 2015
- views: 7899
How to Calibrate a Spectrophotometer with Potassium dichromate
- Order: Reorder
- Duration: 2:36
- Updated: 21 May 2015
- views: 16224
http://www.fireflysci.com/calibration-kits/photometric-linearity-potassium-dichromate-kit
Learn how to calibrate a UV VIS spectrophotometer using potassium dic...
http://www.fireflysci.com/calibration-kits/photometric-linearity-potassium-dichromate-kit
Learn how to calibrate a UV VIS spectrophotometer using potassium dichromate calibration filters.
I’m going to turn on our spectrophotometer and let it heat up for 45 minutes.
Let’s go ahead and choose a wavelength to calibrate. For potassium dichromate there are four different wavelengths you can choose. I’m going to set our machine to test 235nm.
Now we have to insert the perchloric acid blank that came with our potassium dichromate kit. When we insert the blank we’re going to get a reading, so we have to blank the machine now with the blank still in the cuvette holder.
When the machine is reading zero we can insert one of the potassium dichromate filters. Let’s go with the 20 mg/L filter. Now we’ll check the reading against the certificate of calibration.
Let’s go ahead and test 40 mg/L at 235 nm.
When you switch to different wavelengths, you have to insert the blank and blank the machine.
The reason we use a blank is because we only want to measure the absorbance of the potassium dichromate and not the cuvette or the perchloric acid. The blank accounts for the percholic acid and the cuvette so when we zero the machine we are eliminating these factors and leaving us only with the potassium dichromate.
Remember to check your certificate of calibration for the tolerance of your potassium dichromate kit. Also check your spectrophotometers manual to find the photometric accuracy tolerance of your machine and add this to the tolerance on the certificate of calibration.
Thanks for taking the time to watch this video. Please give us a thumbs up and leave us your questions and comments below.
If you need potassium dichromate filters you can visit our website at fireflysci.com and look for our calibration kits.
See ya next time!
https://wn.com/How_To_Calibrate_A_Spectrophotometer_With_Potassium_Dichromate
http://www.fireflysci.com/calibration-kits/photometric-linearity-potassium-dichromate-kit
Learn how to calibrate a UV VIS spectrophotometer using potassium dichromate calibration filters.
I’m going to turn on our spectrophotometer and let it heat up for 45 minutes.
Let’s go ahead and choose a wavelength to calibrate. For potassium dichromate there are four different wavelengths you can choose. I’m going to set our machine to test 235nm.
Now we have to insert the perchloric acid blank that came with our potassium dichromate kit. When we insert the blank we’re going to get a reading, so we have to blank the machine now with the blank still in the cuvette holder.
When the machine is reading zero we can insert one of the potassium dichromate filters. Let’s go with the 20 mg/L filter. Now we’ll check the reading against the certificate of calibration.
Let’s go ahead and test 40 mg/L at 235 nm.
When you switch to different wavelengths, you have to insert the blank and blank the machine.
The reason we use a blank is because we only want to measure the absorbance of the potassium dichromate and not the cuvette or the perchloric acid. The blank accounts for the percholic acid and the cuvette so when we zero the machine we are eliminating these factors and leaving us only with the potassium dichromate.
Remember to check your certificate of calibration for the tolerance of your potassium dichromate kit. Also check your spectrophotometers manual to find the photometric accuracy tolerance of your machine and add this to the tolerance on the certificate of calibration.
Thanks for taking the time to watch this video. Please give us a thumbs up and leave us your questions and comments below.
If you need potassium dichromate filters you can visit our website at fireflysci.com and look for our calibration kits.
See ya next time!
- published: 21 May 2015
- views: 16224
Spectrophotometer Use
- Order: Reorder
- Duration: 2:50
- Updated: 13 Jun 2017
- views: 0
- published: 13 Jun 2017
- views: 0
Spectrophotometer
- Order: Reorder
- Duration: 1:32
- Updated: 24 Nov 2009
- views: 14529
How to use the spectrophotometer correctly
How to use the spectrophotometer correctly
https://wn.com/Spectrophotometer
How to use the spectrophotometer correctly
- published: 24 Nov 2009
- views: 14529
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0:59
How does a spectrophotometer work?
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published: 04 Nov 2011
How does a spectrophotometer work?
How does a spectrophotometer work?
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- published: 04 Nov 2011
- views: 288735
5:44
How To Use A Spectrophotometer
published: 04 Mar 2014
How To Use A Spectrophotometer
How To Use A Spectrophotometer
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- published: 04 Mar 2014
- views: 70966
6:27
The Spectrophotometer: A demo and practice experiment
The spectrophotometer is an instrument used to measure the effect of a sample on a beam of...
published: 07 Nov 2011
The Spectrophotometer: A demo and practice experiment
The Spectrophotometer: A demo and practice experiment
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- published: 07 Nov 2011
- views: 157705
13:06
Spectrophotometry introduction | Kinetics | Chemistry | Khan Academy
Spectrophotometry, transmittance, absorbance and the Beer-Lambert Law. Created by Sal Khan...
published: 15 Oct 2010
Spectrophotometry introduction | Kinetics | Chemistry | Khan Academy
Spectrophotometry introduction | Kinetics | Chemistry | Khan Academy
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- published: 15 Oct 2010
- views: 285040
6:48
How a Simple UV-visible Spectrophotometer Works
Professor Davis describes a simple example of a double-beam UV-visible spectrophotometer a...
published: 29 Sep 2014
How a Simple UV-visible Spectrophotometer Works
How a Simple UV-visible Spectrophotometer Works
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- published: 29 Sep 2014
- views: 116275
3:42
TRU Chemistry Labs: How To Calibrate and use the Spectrophotometer
This video goes over the calibration and use of the Genesys 20 spectrophotometer used in t...
published: 04 Sep 2014
TRU Chemistry Labs: How To Calibrate and use the Spectrophotometer
TRU Chemistry Labs: How To Calibrate and use the Spectrophotometer
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- published: 04 Sep 2014
- views: 21497
2:55
How to use a Spectrophotometer
published: 16 Jan 2015
How to use a Spectrophotometer
How to use a Spectrophotometer
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- published: 16 Jan 2015
- views: 7899
2:36
How to Calibrate a Spectrophotometer with Potassium dichromate
http://www.fireflysci.com/calibration-kits/photometric-linearity-potassium-dichromate-kit
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published: 21 May 2015
How to Calibrate a Spectrophotometer with Potassium dichromate
How to Calibrate a Spectrophotometer with Potassium dichromate
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- published: 21 May 2015
- views: 16224
2:50
Spectrophotometer Use
published: 13 Jun 2017
Spectrophotometer Use
Spectrophotometer Use
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- published: 13 Jun 2017
- views: 0
1:32
Spectrophotometer
How to use the spectrophotometer correctly
published: 24 Nov 2009
Spectrophotometer
Spectrophotometer
- Report rights infringement
- published: 24 Nov 2009
- views: 14529
How does a spectrophotometer work?...
How To Use A Spectrophotometer...
The Spectrophotometer: A demo and practice experi...
Spectrophotometry introduction | Kinetics | Chemis...
How a Simple UV-visible Spectrophotometer Works...
TRU Chemistry Labs: How To Calibrate and use the S...
How to use a Spectrophotometer...
How to Calibrate a Spectrophotometer with Potassiu...
Spectrophotometer Use...
