The Rochester Rattlers are a Major League Lacrosse (MLL) professional men's field lacrosse team based in Rochester, New York. They are a charter member of the MLL. From their inaugural season in 2001 to 2005, they were in the National Division. From 2006 to 2008, they were a member of the Eastern Conference.

After the 2008 season, the team was dissolved. The Rattlers' staff and roster was transferred to the new Toronto Nationals franchise; however, the history, colors, and name of the Rattlers franchise remained in Rochester so that they could be used for a future MLL team. On November 23, 2010, the Chicago Machine officially announced they would move to Rochester and resume play in 2011 under the Rochester Rattlers name.

In 2001 and 2002 the team played home games at Frontier Field. They played at Bishop Kearney Field from 2003 to 2005, and moved into the new PAETEC Park in 2006. The Rattlers made the playoffs in 2001 and 2005 as a wildcard. They were the National Division champions in 2004. On June 16, 2007, the Rochester Rattlers won a 27–26 (in overtime) game over the Denver Outlaws in the highest scoring game in MLL history at INVESCO Field at Mile High.

Joel White (1930–1997), the son of author E. B. White and New Yorker Magazine editor Katharine Sergeant Angell White, was a renowned U.S. naval architect known for his classic and beautiful designs including the W-Class of boats. Two W boats were posthumously built by Rockport Marine and Brooklin Boat Yard for Donald Tofias. They were christened White Wings and Wild Horses. White's life and character were chronicled in the book A Unit of Water, A Unit of Time by Douglass Whynott and in Joel White: Boatbuilder / designer / sailor by Bill Mayher and Maynard Bray. White died at the age of 66 in 1997 in Brooklin, Maine of lung cancer. His widow, Allene White, still lives in Brooklin along with two of his sons and their families.