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This is a list of fictional characters who appear in the Mario franchise of video games developed by Nintendo, as well as spin-off media, such as books, comics, and animated series.

Pauline (ポリーン, Porīn^?) is the damsel in distress and heroine of the original Donkey Kong, as well as the 1994 Game Boy game of the same name. She also appeared in Mario vs. Donkey Kong 2: March of the Minis and Mario vs. Donkey Kong: Minis March Again.

In the original Donkey Kong, she is held captive by Donkey Kong and it is Mario's objective to climb up the construction site and rescue her. Along the way, the player can collect feminine articles (a hat, purse, and parasol) that Pauline drops for bonus points. In the same way that Mario was originally called Jumpman in Japan, Pauline was originally known as Lady (レディ, Redi^?). The name Pauline was chosen for the character during the game's distribution in North America after Polly James, the wife of Nintendo of America's warehouse manager, Don James.^{[citation needed]} After Donkey Kong, Pauline made cameo appearances in two NES games, Pinball and Famicom BASIC.Princess Peach, introduced in Super Mario Bros., supplanted Pauline's original role as damsel in distress in the Mario games.