Cpg Islands

GENETICS 3: EPIGENETICS: CpG ISLANDS

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Duration: 1:16
Updated: 20 Aug 2012
views: 7359

EPIGENETICS: CpG ISLANDS

wn.com/Genetics 3 Epigenetics Cpg Islands

CpG islands

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Duration: 2:23
Updated: 15 Dec 2013
views: 3085

This tutorial about DNA methylation explains the CpG island found in vertibrate genome specific hotspot sequences for DNA methylation. For more information, log on to- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html PPT source- Dr. Anindita Banerjee, Department of Microbiology, St. Xavier's college

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CpG Islands Lec - Intro to Bioinformatics (UCLA)

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Duration: 4:01
Updated: 19 Nov 2015
views: 167

wn.com/Cpg Islands Lec Intro To Bioinformatics (Ucla)

How Methylation Silences Genes

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Duration: 0:44
Updated: 29 Mar 2012
views: 52800

When CpG islands are methylated, a gene may be repressed. I am not the creator of this video. All credit is due to HHMI.

wn.com/How Methylation Silences Genes

CpG island identification with hidden Markov models for DNA sequence analysis

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Duration: 63:42
Updated: 24 Jun 2014
views: 687

Raina Robeva (Sweet Briar College) gave a talk entitled "CpG island identification with hidden Markov models for DNA sequence analysis" at the Algebraic and Discrete Biological Models for Undergraduate Courses Tutorial held at NIMBioS June 18-20, 2014. To read more about the tutorial, click the following link: http://nimbios.org/tutorials/TT_mathbio

wn.com/Cpg Island Identification With Hidden Markov Models For Dna Sequence Analysis

Epigenetics in a Minute

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Duration: 2:45
Updated: 20 Mar 2015
views: 1609

Once upon a time in the world of genetics, two identical twins were born. The two baby boys were named Jim and Tim Van Horn. All observable phenotypes were alike, this was no surprise because they were genetic clones. Their hair, eyes, behaviours were the same– including all their milestones. My oh my , they were definitely twin brothers!! Then one day their worlds changed when mommy and daddy stopped loving each other. No longer wanted to be husband and wife, moved to two different cities and started a new life. Tim lived with mom and Jim lived with dad. Different environments, different experiences for both of the lads. As time went on, the most unfortunate thing happened. They contracted different illnesses, both hospitalized and saddened. But how was this possible? They were identical you know, same genotype means same phenotypes no? What was this phenomenon that caused this alteration? Epigenetics was the reason, specifically, methylation! Tim and Jim differ epigenetically but have the identical DNA. What is this epigenetics I keep on speaking of you might say? All the cells in our bodies have the same DNA but necessarily don’t know how to behave Epigenetics provides instructions so the cells know what to do, for example, they’ll follow the muscle cell lineage and will be a muscle cell slave These instructions are provided through a chemical reaction known as methylation. Carbon and hydrogen form methyl groups, this small molecule is all that’s needed for these alterations Methylation is the covalent addition of methyl groups to DNA or histone protein, governing and directing dna transcription like a university dean Specifically methyl groups target CpG islands, attaching to a cytosine residue This stuff is fascinating, I know you’re excited too (tone change) Methylation can silence the gene by hiding the promoter sequence Preventing the binding of the RNa polymerase and eliminating transcription frequence It can also cause overexpression of a certain gene, leading to a phenotype that shouldn’t be seen Cancers and obesity just to name a few, these endless epigenetics issues can even affect you Now let’s see what happened to Jim who suffers from esophageal cancer. How is methylation going to provide the answer? The FHIT gene prevents over proliferation of epithelium cells. But since Jim was a smoker, the nicotine in his system induced FHIT methylation spells. Epithelial cells were no longer under control, always being rowdy and continuously on the roll. The cancer grew unrestricted, nothing to stop the esophagus from being constricted. Moving onto Tim, let’s talk about what happened to him Eating a nutrient poor diet, lead him on an obesity riot. Lack of methyl groups from hamburgers, fries, and shakes was certain, Resulting in the overexpression of obesity-linked genes known as TNF-Alpha and Leptin So you see that Jim and Tim had methylation differences induced by environmental factors, we can say genetics no longer was the main actor. Even though clones are the same genotypically, methylation can make them look different phenotypically (banana example) I hope you understood Epigentics my friend because this is the part where I say THE END.

wn.com/Epigenetics In A Minute

Medical vocabulary: What does CpG Islands mean

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Duration: 0:30
Updated: 19 Jan 2016
views: 13

What does CpG Islands mean in English?

wn.com/Medical Vocabulary What Does Cpg Islands Mean

What Is The Definition Of CpG island - Medical Dictionary Free Online

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Duration: 0:42
Updated: 25 Sep 2014
views: 226

Visit our website for text version of this Definition and app download. http://medicalschooldictionary.com/ Subjects: medical terminology, medical dictionary, medical dictionary free download, medical terminology made easy, medical terminology song medical dictionary free download how to download medical dictionary stedman's medical dictionary best medical dictionary taber's medical dictionary oxford medical dictionary stedman's electronic medical dictionary medical dictionary free download medical dictionary online medical dictionary free download medical dictionary online medical school interview medical school parody medical school documentary medical school life medical school acceptance medical school motivation medical school interview questions and answers medical school pathology medical school graduation medical school admissions medical school interview medical school parody medical school documentary medical school life medical school acceptance medical school motivation medical school interview questions and answers medical school pathology medical school graduation medical school admissions medical school acceptance medical school anatomy medical school advice medical school application medical school acceptance reaction medical school acceptance letter medical school anatomy lecture medical school admissions committee medical school application process medical school anatomy lab medical school biochemistry medical school biochemistry lecture medical school blog medical school books medical school class medical school cadaver dissection medical school commencement medical school curriculum medical school courses medical school class lecture medical school cost medical school cardiology medical school comedy medical school cadaver medical school documentary medical school debt medical school die in medical school dissection medical school disney parody medical school day in the life medical school dropout medical school dating medical school dic medical school dorm medical school experience medical school electron transport chain medical school exam medical school embryology medical school essentials medical school europe my medical school experience medical school funny medical school first year medical school for international health medical school frozen parody medical school for everyone medical school first day medical school finals ucsf mini medical school for the public is medical school for me getting into medical school for dummies paperback medical school graduation medical school gunner medical school glycolysis made easy medical school gpa medical school graduation ceremony medical school gross anatomy medical school gluconeogenesis medical school group interview medical school glycolysis medical school genetics gw medical school medical school histology medical school harvard medical school how to study medical school humor medical school hard yale medical school hunger games harvard medical school harlem shake medical school interview medical school interview questions and answers medical school interview tell me about yourself medical school inspiration medical school interview attire medical school immunology medical school interview mock medical school interview why do you want to be a doctor medical school in the caribbean medical school in cuba i medical school glycolysis medical school journey medical school john hopkins medical school krebs cycle medical school lecture medical school lecture anatomy medical school let it go medical school lecture harvard medical school lecture biochemistry medical school low gpa medical school look at me now medical school lab what is medical school like john hopkins medical school lectures medical school motivation medical school mock interview medical school mmi medical school musical medical school music video medical school match day 2014 medical school microbiology medical school movie medical school made easy medical school mmi interview u of m medical school medical school notes medical school nurse medical school new zealand medical school oxidative phosphorylation medical school overview medical school of pathology medical school oxford cooper medical school of rowan university manchester medical school osce medical school parody medical school pathology medical school personal statement medical school pathology lectures medical school physiology medical school panel medical school pharmacology medical school physical exam medical school parody let it go medical school prank medical school questions medical school residency medical school rap medical school rotations medical school requirements medical school reaction medical school ragging medical school reality medical school routine medical school revision getting into medical school reaction medical school song medical school study tips medical school studying medical school scholarships

wn.com/What Is The Definition Of Cpg Island Medical Dictionary Free Online

POPOO CPG ISLAND

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Duration: 6:42
Updated: 12 Jul 2009
views: 170

watch it!!!

wn.com/Popoo Cpg Island

Statistics for Genomics: DNA methylation

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Duration: 70:04
Updated: 06 Jul 2012
views: 10040

I briefly explain the biology of DNA methylation, CpG Islands and measurement technology (microarrays and sequencing). I also go over some of recent results made possible with these technologies.

wn.com/Statistics For Genomics Dna Methylation

cpg

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Duration: 10:58
Updated: 10 Mar 2013
views: 129

cpg islands were usefull in using methylated specific primers because those areas become methylated at the start of cancer, and durring metastasis. sodium bisulfate was used to help the methylated specific primer distinguish between the methylated and non methylated dna. from that they were able to determine how much of the samples were methylated and not methylated. methylation is one of the causes (one of the major causes ) of thyroid cancer.

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MB_W16_Lecture20

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Duration: 64:40
Updated: 29 Feb 2016
views: 5

Table of Contents: 00:09 - Clicker 20-1 02:13 - Clicker 20-2 06:31 - Outline Lecture 20 08:21 - Differentiation 10:21 - Master Regulator - Transdifferentiation 11:11 - Ey regulatory cascade: 13:37 - Master Regulator - Transdifferentiation 13:49 - Ey regulatory cascade: 14:06 - IPS cells 18:39 - Coordination of gene expression 18:51 - IPS cells 19:27 - Coordination of gene expression 22:47 - Yeast Mating type switching 22:49 - Coordination of gene expression 22:57 - Yeast Mating type switching 24:24 - Yeast Mating Type Switching 25:04 - Yeast Mating type switching 25:12 - Yeast Mating Type Switching 25:14 - Yeast Mating type switching 25:28 - Yeast Mating Type Switching 28:06 - MAT and HM loci 29:15 - Yeast Mating type maintenance 32:01 - Positive feedback loop 33:44 - Phage Lambda 33:46 - Positive feedback loop 33:52 - Phage Lambda 36:55 - Lambda “Genetic Switch” 38:58 - Gene Regulation Circuits 41:27 - Gene regulatory circuits 43:37 - Sea Urchin development 44:16 - Clock-like oscillators 46:38 - DNA methylation 50:52 - CpG islands 51:05 - DNA methylation 51:08 - CpG islands 54:23 - Why islands? 55:25 - Gene silencing 59:30 - Genomic Imprinting 01:02:13 - Imprinting can activate genes 01:03:44 -

wn.com/Mb W16 Lecture20

DNA Methylation Animation - EpiXplore™ Methylated DNA Enrichment Kit

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Duration: 1:43
Updated: 03 May 2010
views: 29605

Learn more: http://www.clontech.com/US/Products/Cell_Biology_and_Epigenetics/Epigenetics_Kits/DNA_Methylation_Kits/Methylated_DNA_Enrichment To order call: 800.662.2566 or Email: orders@clontech.com Purification and enrichment of CpG methylated DNA by MBD2 (methylated DNA Binding Protein). Ensures enrichment of 95% of the methylated CpG islands. -It does not require any antibodies or follow an immunoprecipitation (IP) protocol. -Its flexible protocol allows you to elute your methylated DNA in fractions (by methylation density) or as a single fraction.

wn.com/Dna Methylation Animation Epixplore™ Methylated Dna Enrichment Kit

Shunsuke Suzuki | Shinshu University | Japan | Genomics- 2014 | OMICS International

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Duration: 23:12
Updated: 22 Jun 2015
views: 34

Title: Emergence of novel CpG island is the key genomic change for the evolution of mammalian genomic imprinting 2nd International Conference on Genomics & Pharmacogenomics September 08-10, 2014 Double Tree by Hilton Hotel Raleigh-Brownstone-University, USA OMICS International: http://omicsonline.org Vaccine Conferences: http://conferenceseries.com/immunology-conferences.php Global Medical Conferences: http://conferenceseries.com Global Pharmaceutical Conferences: http://pharmaceuticalconferences.com Global Cancer Conferences: http://cancersummit.org Global Diabetes Conferences: http://diabetesexpo.com Global Dental Conferences: http://dentalcongress.com Global Nursing Conferences: http://nursingconference.com Abstract Genomic imprinting is an epigenetic mechanism which induces parent-of-origin-dependent expression to subset of genes. In higher vertebrates, genomic imprinting has been found only in viviparous mammals (the eutherians and marsupials) and some imprinted genes have essential functions in fetal and placental development and maternal behavior for post-natal care. Therefore, how genomic imprinting arose during mammalian evolution is of great importance to understand its relevance to the evolution of these mammalian traits. Parent-of-origin-dependent expression of imprinted genes is mostly associated with parental allele-specific DNA methylation of the CpG islands called differentially methylated regions (DMRs). Although the essential role of DMRs for genomic imprinting mechanism has been well established, little is known about how they evolved. Comparative genome analysis in the SGCE-PEG10 domain revealed that PEG10, a retrotransposon-derived imprinted gene essential for placental development, was acquired in the common ancestor of marsupials and eutherians. Furthermore, in the tammar wallaby, both imprinting and differential methylation were restricted to PEG10 unlike eutherians, suggesting that the insertion of PEG10 was the origin of imprinting in this domain. Also, comparative genome analyses in other imprinted domains showed that most DMRs have emerged as novel CpG islands during mammalian evolution. I suppose that the emergence of novel CpG island consequent of retrotransposon insertion was the key genomic change for the acquisition of DMRs that evolved imprinted domains during mammalian evolution. Biography Shunsuke Suzuki has completed his PhD from Tokyo Institute of Technology in 2006 and Postdoctoral studies at Tokyo Medical and Dental University (2006-2009) and The University of Melbourne, Australia (2009-2012). He is now an Assistant Professor (tenure-track) at Epigenomics Division, Frontier Agriscience and Technology Center, Faculty of Agriculture, Shinshu University, Japan. His work is focused on the role of retrotransposons in the evolution of gene regulatory mechanisms in mammals.

wn.com/Shunsuke Suzuki | Shinshu University | Japan | Genomics 2014 | Omics International

Lecture 23: Hidden Markov models

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Duration: 49:25
Updated: 09 Jan 2015
views: 593

Hidden Markov models to identify CpG islands. This lecture follows the discussion in Durbin and Eddy.

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Lecture 24: Hidden Markov models and the Vitterbi algorithm

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Duration: 49:55
Updated: 09 Jan 2015
views: 888

Finish the discussion of HMMs for CpG islands. Introduction to the Vitterbi algorithm (really dynamic programming) to find the most likely Markov Chain generating a given sequence.

wn.com/Lecture 24 Hidden Markov Models And The Vitterbi Algorithm

Dna Methylation AND Decitabine

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Duration: 3:33
Updated: 06 Jun 2014
views: 6957

This av about Dna Methylation AND Decitabine used software AE and PR for this av used on health company...........

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Obtaining Methylation Status Using Digital PCR - ASHG 2014

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Duration: 3:59
Updated: 23 Oct 2014
views: 577

After doing a genome-wide methylation scan in his reproductive biology research, Dr. Matthew Dyson of Northwestern University used the QuantStudio™ 3D Digital PCR System to quantify methylation status in a specific CpG island. He discusses the use of methylation-specific PCR (MSP) compared to a digital PCR-based solution. Learn more about the QuantStudio 3D Digital PCR System: http://www.lifetechnologies.com/us/en/home/life-science/pcr/digital-pcr/quantstudio-3d-digital-pcr-system.html

wn.com/Obtaining Methylation Status Using Digital Pcr Ashg 2014

The Difference between the United Kingdom, Great Britain and England Explained

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Duration: 5:15
Updated: 30 Jan 2011
views: 8482072

Help support videos like this: https://www.patreon.com/cgpgrey T-Shirts: http://store.dftba.com/collections/cgp-grey Grey's blog: http://www.cgpgrey.com/blog/ Twitter: http://twitter.com/cgpgrey

wn.com/The Difference Between The United Kingdom, Great Britain And England Explained

MB_W15_Lecture21

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Duration: 65:03
Updated: 04 Mar 2015
views: 475

Table of Contents: 02:32 - Clicker 21-1 06:04 - Clicker 21-2 09:59 - Clicker/Online Quiz Survey 13:29 - Outline Lecture 21 15:24 - Differentiation 18:51 - Master Regulator 20:20 - Ey regulatory cascade: 25:02 - IPS cells 28:03 - Coordination of gene expression 28:54 - Where enhancers work 28:57 - Coordination of gene expression 29:33 - Where enhancers work 29:36 - Coordination of gene expression 31:22 - Where enhancers work 31:26 - Coordination of gene expression 31:49 - Where enhancers work 35:41 - Positive feedback loop 36:01 - Phage Lambda 36:02 - Lambda “Genetic Switch” 36:04 - Phage Lambda 36:06 - Positive feedback loop 37:30 - Phage Lambda 38:04 - Lambda “Genetic Switch” 40:16 - Gene Regulation Circuits 41:55 - Feed Forward Loop 41:57 - Gene Regulation Circuits 43:18 - Feed Forward Loop 44:51 - DNA methylation 47:36 - CpG islands 49:08 - Why islands? 53:01 - Gene silencing 56:49 - Genomic Imprinting 58:20 - Imprinting can activate genes 59:28 - 01:01:05 - X-inactivation 01:02:23 - X-inactivation mechanism 01:02:49 - Epigenetics 01:02:51 - X-inactivation mechanism 01:03:31 - Epigenetics 01:04:56 - Transcriptional Regulation

wn.com/Mb W15 Lecture21

American Empire

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Duration: 6:24
Updated: 03 Jul 2014
views: 4010550

‡ https://www.youtube.com/watch?v=0dU4IMex4FU http://www.CGPGrey.com/ https://subbable.com/cgpgrey Discuss: http://www.reddit.com/r/CGPGrey/comments/29qrly/american_empire/ Special thanks: Alex Perelgut Keyan Halperin Robin McGhee Dragos Dumitrescu Adam Miller Andrew Hawling YumSubs Bryan Crockett Tom Sommerville Damien Polglase Andrew Escobar Sam Duckworth

wn.com/American Empire

03 Tumor Supressors

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Duration: 19:29
Updated: 30 Oct 2014
views: 1708

A presentation on Tumor suppressor genes and the cellular process that lead to both copies of the gene being perturbed. Tumor suppressor genes A new hypothesis Once these growth-suppressing genes are lost, the proliferation of the cancer cells accelerates no longer being held back by the actions of these growth-suppressing genes. As long as the cancer cell lacks these genes, it continues to proliferate in a malignant fashion. The moment that wild-type, intact versions of these genes operate once again within the cancer cell, the proliferation of the cancer cell grinds abruptly to a halt. The case against the existence of tumor suppressor genes both wild-type copies of a tumor suppressor gene need to be eliminated This requirement for two separate genetic alterations Is this too improbable to occur in a reasonably short period of time. the probability of inactivating a single gene copy by mutation 10-6 per cell generation, the probability of silencing both copies is on the order of 10-12 per cell generation exceedingly unlikely!!! Alfred Knudson concluded in 1971 the rate of appearance of familial tumors was consistent with a single random event the sporadic tumors behaved as if two random events were required for their formation If the tumor predisposing alleles of Rb are recessive, then both copies of the Rb gene must be knocked (unlikely through mutation?) A solution to this dilemma Suppose that the first Rb gene was inactivated by mutation 1 10-6 per cell generation. this heterozygous cell would continue to exhibit a wild-type phenotype. the second, intact Rb is inactivated by an non-mutational event? Mitotic recombination was found to occur at a frequency of up to 10-4 per cell generation. a far easier way for a cell to rid itself of the remaining wild-type copy of the Rb gene than mutational inactivation Loss of heterozygosity (LOH) following the mitotic recombination they lose heterozygosity and become homozygous. Gene Conversion can achieve LOH. A DNA strand being elongated during DNA replication on one chromosome will form a hybrid with the complementary DNA strand present in the second, homologous chromosome. this newly synthesized strand of DNA will acquire DNA sequences from a stretch of the paired chromosome. Such gene conversion is known to occur even more frequently per cell generation than does mitotic recombination. Retinoblastoma tumor cells were predicted to show LOH at the Rb locus only a small minority were predicted to carry two distinct, mutant alleles of Rb, (each inactivated by an independent mutational event) DNA methylation to inactivate TSG’s DNA molecules can be altered covalently by the attachment of methyl groups to cytosine bases. Recent research indicates that this modification of the genomic DNA is as important as mutation in shutting down tumor suppressor genes. In mammalian cells methylation is found only in a position that is 5’ to guanosines, that is, in the sequence CpG. when CpG methylation occurs in the vicinity of a gene promoter, it can cause repression of transcription of the associated gene. blocking gene transcription Protein complexes bind methylated CpGs these function as a histone deacetylase (HDAC) enzymes histone deacetylase (HDAC) remove acetate groups that are attached to the side-chains of histone molecules This converts the chromatin from a configuration favoring transcription to one blocking transcription. DNA acetylation Locally, CpG island methylation increases in cancer cells Globally, DNA methylation decreases through cancer progression “global hypomethylation” can be attributed to the loss of methyl groups attached to the DNA of highly repeated sequences in the cell genome this loss is correlated with chromosomal instability The importance of promoter methylation It has become apparent that promoter methylation is as important in shutting down tumor suppressor genes. In sporadic retinoblastomas Rb gene can be inactivated by somatic mutations or by promoter methylation. This is true for other TSG’s Cancer Genetics in brief series… by Mark Temple

wn.com/03 Tumor Supressors

Suzuki: Methylation Patterns: SMRT Sequencing Data for Haplotype-Specific Epigenetic Analysis

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Duration: 2:36
Updated: 19 May 2015
views: 121

AGBT 2015: Observing Heterozygotic DNA Methylation Patterns in Diploid Genomes Using Kinetics Data From PacBio RS, Yuta Suzuki (University of Tokyo) Yuta Suzuki from the University of Tokyo presents a poster on heterozygotic DNA methylation patterns at AGBT 2015. He used kinetic data from SMRT® Sequencing to generate epigenetic information on samples ranging from human to medaka fish and was able to analyze haplotype-specific methylation data. He also shows that long reads are better able to capture data about CpG islands than short-read sequences.

wn.com/Suzuki Methylation Patterns Smrt Sequencing Data For Haplotype Specific Epigenetic Analysis