Machaquilá refers to a ruined city of the Maya civilization in what is now the El Peten department of Guatemala. There is also a small Guatemalan village several kilometers from the site that bears the name Machaquilá.

Machaquilá flourished in the middle Classic to late Classic era, reaching the height of its power in approximately the ninth century CE. The archaeological remains of the city are located 45 km. northeast of Cancuén and 30 km east to Ceibal, in a relatively isolated region, and the site is notable for its finely sculptured stelas, religious altars, hieroglyph panels, and ornate staircases. There are nine plazas in Machaquilá, along with ceremonial buildings, temples, and a residential complex to the south.

There are several smaller cities surrounding Machaquilá and the nearby San Miguel caves. There is evidence of human habitation at the sites dating from the pre-Classic to the early post-Classic periods. The Guatemalan sites known as Esquipulas, El Pueblito, and Achiotal have E-Groups and Mesoamerican ball courts, although Mayan scholars do not believe these sites were part of a formal state. There is evidence of political and trade relationships between El Pueblito and Machaquilá during the late Classic period, and with Ceibal and Cancuén. Mayans likely used the Machaquilá River as a waterway, as it is one of the main tributaries of the Pasión River.