In music, polyphony is a texture consisting of two or more independent melodic voices, as opposed to music with just one voice (monophony) or music with one dominant melodic voice accompanied by chords (homophony).

Within the context of the Western musical tradition, the term is usually used to refer to music of the late Middle Ages and Renaissance. Baroque forms such as the fugue, which might be called polyphonic, are usually described instead as contrapuntal. Also, as opposed to the species terminology of counterpoint, polyphony was generally either "pitch-against-pitch" / "point-against-point" or "sustained-pitch" in one part with melismas of varying lengths in another (van der Werf, 1997). In all cases the conception was likely what Margaret Bent (1999) calls "dyadic counterpoint", with each part being written generally against one other part, with all parts modified if needed in the end. This point-against-point conception is opposed to "successive composition", where voices were written in an order with each new voice fitting into the whole so far constructed, which was previously assumed.

William Byrd (1540 or late 1539 – 4 July 1623) was an English composer of the Renaissance. He wrote in many of the forms current in England at the time, including various types of sacred and secular polyphony, keyboard (the so-called Virginalist school) and consort music.

Knowledge of Byrd's biography expanded in the late 20th century, thanks largely to the research of John Harley (Harley, 1997). Following the discovery of a document dated 2 October 1598 in which Byrd's age is given as "58 years or there abouts," it now appears that he was born in 1540. The older dating 1542–3 is derived from Byrd's will (endorsed on 22 November 1622) which describes him as 'in the 80th year of my age'. It now becomes clear that it must have been drafted about three years earlier than the date of endorsement. Byrd was born in London, the son of a Thomas Byrd (not Thomas Byrd of the Chapel Royal) about whom little is known. Byrd had two brothers, Symon'd and John, and four sisters. It is clear from a reference in the prefatory material in the Tallis/Byrd Cantiones of 1575 that Byrd was a pupil of Thomas Tallis, then the leading composing member of the Chapel Royal Choir. Byrd also worked in collaboration with two other Chapel Royal singing-men, John Sheppard and William Mundy, on one of his earliest compositions, a contribution to a joint setting of the alternatim psalm In exitu Israel composed for the procession to the font at the Paschal Vigil. As an item for the Sarum liturgy this was presumably composed near the end of the reign of Mary Tudor (1553–1558), whose Catholic beliefs impelled her to revive Sarum liturgical practices during her brief reign. In view of these contacts it is reasonable to speculate that Byrd was a Chapel Royal choirboy, though the surviving records do not name the choristers individually.

Giovanni Pierluigi da Palestrina (3 February 1525 or 2 February 1526 – 2 February 1594) was an Italian Renaissance composer of sacred music and the best-known 16th-century representative of the Roman School of musical composition. He had a lasting influence on the development of church music, and his work has often been seen as the culmination of Renaissance polyphony.

Palestrina was born in the town of Palestrina, near Rome, then part of the Papal States. Documents suggest that he first visited Rome in 1537, when he is listed as a chorister at the Santa Maria Maggiore basilica. He studied with Robin Mallapert and Firmin Lebel. He spent most of his career in the city.

Palestrina came of age as a musician under the influence of the northern European style of polyphony, which owed its dominance in Italy primarily to two influential Franco-Flemish composers, Guillaume Dufay and Josquin des Prez, who had spent significant portions of their careers there. Italy itself had yet to produce anyone of comparable fame or skill in polyphony.

Duarte Lobo (c. 1565 – 24 September 1646; Latinized as Eduardus Lupus) was a Portuguese composer of the late Renaissance and early Baroque. He was one of the most famous Portuguese composers of the time, together with Filipe de Magalhães, Manuel Cardoso, composers who all began their academic studies as students of Manuel Mendes. Along with John IV, King of Portugal, they represent the "golden age" of Portuguese polyphony.

Details of his life are sparse. He was born in Alcáçovas, in Alentejo, southern Portugal. He is known to have been a choir boy at Évora where he subsequently studied with Manuel Mendes . His first position was as mestre de capela of the cathedral of Évora; sometime before 1589, he became maestro di cappella at the Hospital Real, Lisbon. By 1591 he was appointed as mestre de capela at the cathedral in Lisbon, a position he held till 1639. This was the most prestigious musical appointment in the country.

He also served as director of the Seminar of St. Bartholomew, and was also a professor of music at the 'Colégio do Claustro da Sé' (College at the Holy See Cloisters) in Lisbon, where he taught Manuel Machado.

Thomas Tallis (c. 1505 – 23 November 1585) was an English composer. Tallis flourished as a church musician in 16th century Tudor England. He occupies a primary place in anthologies of English church music, and is considered among the best of England's early composers. He is honoured for his original voice in English musicianship. No contemporary portrait of Tallis survives: the earliest, painted by Gerard van der Gucht, dates from 150 years after Tallis died, and there is no certainty that it is a likeness.

Little is known about Tallis's early life, but there seems to be agreement that he was born in the early 16th century, toward the close of the reign of Henry VII. Little is known about Tallis's childhood and his significance with music at that age. However, there are suggestions that he was a child of the chapel royal St. James's palace, the same singing establishment which he later went to as a man. His first known appointment to a musical position was as organist of Dover Priory in 1530–31, a Benedictine priory at Dover (now Dover College) in 1532. His career took him to London, then (probably in the autumn of 1538) to the Augustinian abbey of Holy Cross at Waltham until the abbey was dissolved in 1540. Tallis acquired a volume at the dissolution of the monastery of Waltham Holy Cross and preserved it; one of the treatises in it was by Leonel Power, and the treatise itself prohibits consecutive unisons, fifths, and octaves.