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Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9 -
Genome Editing with CRISPR-Cas9
Genome Editing with CRISPR-Cas9Genome Editing with CRISPR-Cas9
This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, including the potential to treat human genetic disease. Feng Zhang, a leader in the development of this technology, is a faculty member at MIT, an investigator at the McGovern Institute for Brain Research, and a core member of the Broad Institute. Further information can be found on Prof. Zhang’s website at http://zlab.mit.edu . Images and footage courtesy of Sputnik Animation, the Broad Institute of MIT and Harvard, Justin Knight and pond5. -
Quick learning of CRISPR/Cas9
Quick learning of CRISPR/Cas9Quick learning of CRISPR/Cas9
What is CRISPR/Cas9? How does CRISPR system work? What is needed to perform genome engineering in your lab? This short video introduces the basics of this no... -
CRISPR Cas9: A novel approach to genetic engineering
CRISPR Cas9: A novel approach to genetic engineeringCRISPR Cas9: A novel approach to genetic engineering
Provides the basic mechanism behind the CRISPR Cas9 complex, while describing application and need. -
CRISPR Cas9 - a Brief Introduction
CRISPR Cas9 - a Brief IntroductionCRISPR Cas9 - a Brief Introduction
CRISPR-cas9 system is a versatile and robust genome editing system with showing success in various model organisms. In this video, you will be introduced to this technology and its various applications and its impact on genome engineering. for more information please visit our website: http://goo.gl/jaKURP -
CRISPR Webinar by Drs. Barrangon, Zhang and Church
CRISPR Webinar by Drs. Barrangon, Zhang and ChurchCRISPR Webinar by Drs. Barrangon, Zhang and Church
Webinar sponsored by OriGene and held by GEN, Genetic Engineering & Biotechnology News This webinar on the genome editing system CRISPR/Cas9 is given by thre... -
The CRISPR-Cas9 System Part 1
The CRISPR-Cas9 System Part 1The CRISPR-Cas9 System Part 1
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering. -
The CRISPR-Cas9 System Part 2
The CRISPR-Cas9 System Part 2The CRISPR-Cas9 System Part 2
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering. -
CRISPR Genome Editing - Horizon Discovery
CRISPR Genome Editing - Horizon DiscoveryCRISPR Genome Editing - Horizon Discovery
Horizon Discovery GENASSIST™ - CRISPR (clustered regularly interspaced short palindromic repeats) is a microbial 'immune system' that has evolved to protect ... -
CRISPR-Cas genome surveillance: From basic biology to transformative technology
CRISPR-Cas genome surveillance: From basic biology to transformative technologyCRISPR-Cas genome surveillance: From basic biology to transformative technology
CRISPR-Cas genome surveillance: From basic biology to transformative technology Air date: Wednesday, March 11, 2015, 3:00:00 PM Category: WALS - Wednesday Afternoon Lectures Runtime: 01:05:13 Description: Wednesday Afternoon Lecture Series Margaret Pittman Lecture Dr. Doudna, who specializes in the study of RNA, will present a brief history of the bacterial RNA-guided CRISPR biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism. Using CRISPR-Cas "clustered regularly interspaced short palindromic repeats" technology provides the foundation for remarkable developments in modifying, regulating, o -
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GECRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
The CRISPR-Cas9 system has revolutionized genetic engineering. This groundbreaking discovery by scientists Jennifer Doudna and Emmanuelle Charpentier has brought medical research light-years closer to finding a cure for many diseases, including sickle cell anemia, HIV, cancer, Huntington's disease and more. Subscribe to the GE Channel: http://invent.ge/1eGgvZK GE works on things that matter. The best people and the best technologies taking on the toughest challenges. Finding solutions in energy, health and home, transportation and finance. Building, powering, moving and curing the world. Not just imagining. Doing. GE works. Connect with G -
WOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New Technology
WOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New TechnologyWOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New Technology
CRISPR/Cas-9 is a new genomic editing system that has the potential to revolutionize basic biomedical research and, possibly, the treatment of genetic disease. The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system utilizes guide RNA as a gene-homing mechanism and a Cas-9 nuclease to cut targeted DNA. As such, it drastically reduces the time-to-results and design complexity associated with existing genomic editing tools. Given CRISPR's simplicity and widespread applicability in biomedical research, scientists and university technology licensing officers are understandably asking about intellectual property rights and a -
CRISPR technology for genome editing
CRISPR technology for genome editingCRISPR technology for genome editing
CRISPR, a pioneering genome-editing technique, technology will allow AstraZeneca to identify and validate new drug targets in preclinical models that closely resemble human disease. -
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
This is a video protocol for generating genomic deletions in mammalian cells lines using CRISPR/Cas9. Bauer, D. E., Canver, M. C., Orkin, S. H. Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. J. Vis. Exp. (95), e52118, doi:10.3791/52118 (2015).
-
Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
http://www.ibiology.org/ibiomagazine/jennifer-doudna-genome-engineering-with-crispr-cas9-birth-of-a-breakthrough-technology.html Talk Overview: Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studie -
Genome Editing with CRISPR-Cas9
Genome Editing with CRISPR-Cas9Genome Editing with CRISPR-Cas9
This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, including the potential to treat human genetic disease. Feng Zhang, a leader in the development of this technology, is a faculty member at MIT, an investigator at the McGovern Institute for Brain Research, and a core member of the Broad Institute. Further information can be found on Prof. Zhang’s website at http://zlab.mit.edu . Images and footage courtesy of Sputnik Animation, the Broad Institute of MIT and Harvard, Justin Knight and pond5. -
Quick learning of CRISPR/Cas9
Quick learning of CRISPR/Cas9Quick learning of CRISPR/Cas9
What is CRISPR/Cas9? How does CRISPR system work? What is needed to perform genome engineering in your lab? This short video introduces the basics of this no... -
CRISPR Cas9: A novel approach to genetic engineering
CRISPR Cas9: A novel approach to genetic engineeringCRISPR Cas9: A novel approach to genetic engineering
Provides the basic mechanism behind the CRISPR Cas9 complex, while describing application and need. -
CRISPR Cas9 - a Brief Introduction
CRISPR Cas9 - a Brief IntroductionCRISPR Cas9 - a Brief Introduction
CRISPR-cas9 system is a versatile and robust genome editing system with showing success in various model organisms. In this video, you will be introduced to this technology and its various applications and its impact on genome engineering. for more information please visit our website: http://goo.gl/jaKURP -
CRISPR Webinar by Drs. Barrangon, Zhang and Church
CRISPR Webinar by Drs. Barrangon, Zhang and ChurchCRISPR Webinar by Drs. Barrangon, Zhang and Church
Webinar sponsored by OriGene and held by GEN, Genetic Engineering & Biotechnology News This webinar on the genome editing system CRISPR/Cas9 is given by thre... -
The CRISPR-Cas9 System Part 1
The CRISPR-Cas9 System Part 1The CRISPR-Cas9 System Part 1
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering. -
The CRISPR-Cas9 System Part 2
The CRISPR-Cas9 System Part 2The CRISPR-Cas9 System Part 2
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering. -
CRISPR Genome Editing - Horizon Discovery
CRISPR Genome Editing - Horizon DiscoveryCRISPR Genome Editing - Horizon Discovery
Horizon Discovery GENASSIST™ - CRISPR (clustered regularly interspaced short palindromic repeats) is a microbial 'immune system' that has evolved to protect ... -
CRISPR-Cas genome surveillance: From basic biology to transformative technology
CRISPR-Cas genome surveillance: From basic biology to transformative technologyCRISPR-Cas genome surveillance: From basic biology to transformative technology
CRISPR-Cas genome surveillance: From basic biology to transformative technology Air date: Wednesday, March 11, 2015, 3:00:00 PM Category: WALS - Wednesday Afternoon Lectures Runtime: 01:05:13 Description: Wednesday Afternoon Lecture Series Margaret Pittman Lecture Dr. Doudna, who specializes in the study of RNA, will present a brief history of the bacterial RNA-guided CRISPR biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism. Using CRISPR-Cas "clustered regularly interspaced short palindromic repeats" technology provides the foundation for remarkable developments in modifying, regulating, o -
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GECRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
The CRISPR-Cas9 system has revolutionized genetic engineering. This groundbreaking discovery by scientists Jennifer Doudna and Emmanuelle Charpentier has brought medical research light-years closer to finding a cure for many diseases, including sickle cell anemia, HIV, cancer, Huntington's disease and more. Subscribe to the GE Channel: http://invent.ge/1eGgvZK GE works on things that matter. The best people and the best technologies taking on the toughest challenges. Finding solutions in energy, health and home, transportation and finance. Building, powering, moving and curing the world. Not just imagining. Doing. GE works. Connect with G -
WOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New Technology
WOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New TechnologyWOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New Technology
CRISPR/Cas-9 is a new genomic editing system that has the potential to revolutionize basic biomedical research and, possibly, the treatment of genetic disease. The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system utilizes guide RNA as a gene-homing mechanism and a Cas-9 nuclease to cut targeted DNA. As such, it drastically reduces the time-to-results and design complexity associated with existing genomic editing tools. Given CRISPR's simplicity and widespread applicability in biomedical research, scientists and university technology licensing officers are understandably asking about intellectual property rights and a -
CRISPR technology for genome editing
CRISPR technology for genome editingCRISPR technology for genome editing
CRISPR, a pioneering genome-editing technique, technology will allow AstraZeneca to identify and validate new drug targets in preclinical models that closely resemble human disease. -
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
This is a video protocol for generating genomic deletions in mammalian cells lines using CRISPR/Cas9. Bauer, D. E., Canver, M. C., Orkin, S. H. Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. J. Vis. Exp. (95), e52118, doi:10.3791/52118 (2015). -
Ethics of CRISPR Gene Editing in Human Stem Cells
Ethics of CRISPR Gene Editing in Human Stem CellsEthics of CRISPR Gene Editing in Human Stem Cells
*These are the viewpoints of the authors alone and not the HIA or UMBC. *We come from a perspective of supporting CRISPR and its use in research. *This is a an exploratory thinking/sketch argument to start a dialogue. As such it is very simplistic, and we recognize that. *This is meant for a lay audience mostly, so most of the fine-detailed science and philosophy is glossed over, this is on purpose, but it does not dilute the content. *There is no "conspiracy to create the movie GATTICA". Many of these techniques are in their infancy, and one should be very careful to not rush to conclusions/judgements. However, it is time to begin a dia -
Antibodies Part 1: CRISPR
Antibodies Part 1: CRISPRAntibodies Part 1: CRISPR
In 2012, scientists had a realization: hidden inside one of the world’s smallest organisms, was one of the world’s most powerful tools. -
LABTalks: CRISPR/Cas9: Simple & versatile genome editing tool
LABTalks: CRISPR/Cas9: Simple & versatile genome editing toolLABTalks: CRISPR/Cas9: Simple & versatile genome editing tool
Dr. Namritha Ravinder discusses the powerful new CRISPR/ Cas9 technology for genome editing as part of our LABTalks series. -
Feng Zhang Development and Applications of CRISPR Cas9 for Genome Editing
Feng Zhang Development and Applications of CRISPR Cas9 for Genome EditingFeng Zhang Development and Applications of CRISPR Cas9 for Genome Editing
Watch on LabRoots at http://labroots.com/user/webinars/details/id/332 The Cas9 endonuclease from the microbial adaptive immune system CRISPR can be easily pr... -
Rewriting the Genome Using CRISPR and Synthetic Biology
Rewriting the Genome Using CRISPR and Synthetic BiologyRewriting the Genome Using CRISPR and Synthetic Biology
The CRISPR/Cas9 system has emerged as one of the leading tools for modifying genomes of organisms ranging from E. coli to humans. Additionally, the simple gene targeting mechanism of CRISPR technology has been modified and adapted to other applications that include gene regulation, detection of intercellular trafficking, and pathogen detection. With a wealth of methods for introducing Cas9 and gRNAs into cells, it can be challenging to decide where to start. In this presentation, Dr Adam Clore describes the CRISPR mechanism and some of the most prominent uses for CRISPR, along with methods where IDT technologies can assist scientists in desig -
CSHL Keynote: Dr. Jennifer Doudna, HHMI/University of California, Berkeley
CSHL Keynote: Dr. Jennifer Doudna, HHMI/University of California, BerkeleyCSHL Keynote: Dr. Jennifer Doudna, HHMI/University of California, Berkeley
"Programmable RNA-guided genome defense in bacteria" from the 2012 Regulatory & Non-Coding RNAs meeting. -
2) CRISPR Cas9 - Methods and Tools
2) CRISPR Cas9 - Methods and Tools2) CRISPR Cas9 - Methods and Tools
In our previous video we introduced the CRISPR/Cas9 system briefly; here we will go over the methods and tools available to you for using the CRISPR/Cas9 system in practice. We invite you to watch our previous video first before continuing with this one: https://www.youtube.com/watch?v=1aJxXWkE3Ek for more information please visit our website: http://www.abmgood.com/iCRISPR-Cas9-sgRNA.html -
3) CRISPR Cas9 - gRNA Design
3) CRISPR Cas9 - gRNA Design3) CRISPR Cas9 - gRNA Design
In our previous videos we introduced the CRISPR Cas9 system briefly (http://youtu.be/1aJxXWkE3Ek) and presented the different tools and methods available for their expression in living systems (http://youtu.be/INC_kdr7I34); here we will go over the design criteria of the gRNA and introduce you to the online programs that simplify this procedure. We invite you to watch our previous videos first before continuing with this one. Chop Chop Harvard: https://chopchop.rc.fas.harvard.edu/ gRNA Design Knowledge Base: Coming Soon! -
Webinar: Genome Editing with CRISPR/Cas9
Webinar: Genome Editing with CRISPR/Cas9Webinar: Genome Editing with CRISPR/Cas9
OriGene offers CRISPR/Cas9, the latest and effective tool for genome editing. This webinar covers the basics of the CRISPR/Cas9 system, as well as the applic...
Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
- Order: Reorder
- Duration: 16:42
- Updated: 23 Mar 2015
http://www.ibiology.org/ibiomagazine/jennifer-doudna-genome-engineering-with-crispr-cas9-birth-of-a-breakthrough-technology.html
Talk Overview:
Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studies that were previously unachievable.
Speaker Biography:
Jennifer Doudna is Professor of the Departments of Chemistry and of Molecular and Cell Biology at University of California, Berkeley, and an Investigator of the Howard Hughes Medical Institute. Early in her career, she studied the structure and mechanism of ribozymes (enzymatic RNA molecules) and RNA-protein complexes, such as the signal recognition particle. Now her research focuses on understanding how RNA molecules control gene expression in bacteria, through the CRISPR-Cas9 system, and eukaryotic cells, through RNA interference and microRNA-mediated translational control. For her outstanding scientific contributions, she was elected into the American Academy of Arts and Sciences in 2002 and the National Academy of Sciences in 2003, and was awarded the 2015 Breakthrough Prize in the Life Sciences.
http://wn.com/Jennifer_Doudna_(UC_Berkeley_HHMI)_Genome_Engineering_with_CRISPR-Cas9
http://www.ibiology.org/ibiomagazine/jennifer-doudna-genome-engineering-with-crispr-cas9-birth-of-a-breakthrough-technology.html
Talk Overview:
Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studies that were previously unachievable.
Speaker Biography:
Jennifer Doudna is Professor of the Departments of Chemistry and of Molecular and Cell Biology at University of California, Berkeley, and an Investigator of the Howard Hughes Medical Institute. Early in her career, she studied the structure and mechanism of ribozymes (enzymatic RNA molecules) and RNA-protein complexes, such as the signal recognition particle. Now her research focuses on understanding how RNA molecules control gene expression in bacteria, through the CRISPR-Cas9 system, and eukaryotic cells, through RNA interference and microRNA-mediated translational control. For her outstanding scientific contributions, she was elected into the American Academy of Arts and Sciences in 2002 and the National Academy of Sciences in 2003, and was awarded the 2015 Breakthrough Prize in the Life Sciences.
- published: 23 Mar 2015
- views: 14
Genome Editing with CRISPR-Cas9
- Order: Reorder
- Duration: 4:13
- Updated: 05 Nov 2014
This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, including the potential to treat human genetic disease. Feng Zhang, a leader in the development of this technology, is a faculty member at MIT, an investigator at the McGovern Institute for Brain Research, and a core member of the Broad Institute. Further information can be found on Prof. Zhang’s website at http://zlab.mit.edu .
Images and footage courtesy of Sputnik Animation, the Broad Institute of MIT and Harvard, Justin Knight and pond5.
http://wn.com/Genome_Editing_with_CRISPR-Cas9
This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, including the potential to treat human genetic disease. Feng Zhang, a leader in the development of this technology, is a faculty member at MIT, an investigator at the McGovern Institute for Brain Research, and a core member of the Broad Institute. Further information can be found on Prof. Zhang’s website at http://zlab.mit.edu .
Images and footage courtesy of Sputnik Animation, the Broad Institute of MIT and Harvard, Justin Knight and pond5.
- published: 05 Nov 2014
- views: 141
Quick learning of CRISPR/Cas9
- Order: Reorder
- Duration: 4:20
- Updated: 04 Sep 2014
- author: OriGene Technologies Inc.
What is CRISPR/Cas9? How does CRISPR system work? What is needed to perform genome engineering in your lab? This short video introduces the basics of this no...
http://wn.com/Quick_learning_of_CRISPR_Cas9
What is CRISPR/Cas9? How does CRISPR system work? What is needed to perform genome engineering in your lab? This short video introduces the basics of this no...
- published: 07 May 2014
- views: 12575
- author: OriGene Technologies Inc.
CRISPR Cas9: A novel approach to genetic engineering
- Order: Reorder
- Duration: 5:06
- Updated: 03 Sep 2014
- author: Walter Wang
Provides the basic mechanism behind the CRISPR Cas9 complex, while describing application and need.
http://wn.com/CRISPR_Cas9_A_novel_approach_to_genetic_engineering
Provides the basic mechanism behind the CRISPR Cas9 complex, while describing application and need.
- published: 23 Apr 2014
- views: 7899
- author: Walter Wang
CRISPR Cas9 - a Brief Introduction
- Order: Reorder
- Duration: 6:22
- Updated: 31 Dec 2014
CRISPR-cas9 system is a versatile and robust genome editing system with showing success in various model organisms. In this video, you will be introduced to this technology and its various applications and its impact on genome engineering. for more information please visit our website: http://goo.gl/jaKURP
http://wn.com/CRISPR_Cas9_-_a_Brief_Introduction
CRISPR-cas9 system is a versatile and robust genome editing system with showing success in various model organisms. In this video, you will be introduced to this technology and its various applications and its impact on genome engineering. for more information please visit our website: http://goo.gl/jaKURP
- published: 31 Dec 2014
- views: 26
CRISPR Webinar by Drs. Barrangon, Zhang and Church
- Order: Reorder
- Duration: 73:19
- Updated: 30 Aug 2014
- author: OriGene Technologies Inc.
Webinar sponsored by OriGene and held by GEN, Genetic Engineering & Biotechnology News This webinar on the genome editing system CRISPR/Cas9 is given by thre...
http://wn.com/CRISPR_Webinar_by_Drs._Barrangon,_Zhang_and_Church
Webinar sponsored by OriGene and held by GEN, Genetic Engineering & Biotechnology News This webinar on the genome editing system CRISPR/Cas9 is given by thre...
- published: 23 Apr 2014
- views: 3984
- author: OriGene Technologies Inc.
The CRISPR-Cas9 System Part 1
- Order: Reorder
- Duration: 11:10
- Updated: 01 Mar 2015
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering.
http://wn.com/The_CRISPR-Cas9_System_Part_1
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering.
- published: 01 Mar 2015
- views: 10
The CRISPR-Cas9 System Part 2
- Order: Reorder
- Duration: 14:33
- Updated: 01 Mar 2015
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering.
http://wn.com/The_CRISPR-Cas9_System_Part_2
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering.
- published: 01 Mar 2015
- views: 6
CRISPR Genome Editing - Horizon Discovery
- Order: Reorder
- Duration: 1:39
- Updated: 27 Aug 2014
- author: GENESIS Gene-editing
Horizon Discovery GENASSIST™ - CRISPR (clustered regularly interspaced short palindromic repeats) is a microbial 'immune system' that has evolved to protect ...
http://wn.com/CRISPR_Genome_Editing_-_Horizon_Discovery
Horizon Discovery GENASSIST™ - CRISPR (clustered regularly interspaced short palindromic repeats) is a microbial 'immune system' that has evolved to protect ...
- published: 08 Apr 2014
- views: 8189
- author: GENESIS Gene-editing
CRISPR-Cas genome surveillance: From basic biology to transformative technology
- Order: Reorder
- Duration: 65:13
- Updated: 16 Mar 2015
CRISPR-Cas genome surveillance: From basic biology to transformative technology
Air date: Wednesday, March 11, 2015, 3:00:00 PM
Category: WALS - Wednesday Afternoon Lectures
Runtime: 01:05:13
Description: Wednesday Afternoon Lecture Series
Margaret Pittman Lecture
Dr. Doudna, who specializes in the study of RNA, will present a brief history of the bacterial RNA-guided CRISPR biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism. Using CRISPR-Cas "clustered regularly interspaced short palindromic repeats" technology provides the foundation for remarkable developments in modifying, regulating, or marking genomic loci in a wide variety of cells and organisms. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way to fundamental discoveries in biology with applications in all branches of biotechnology, and strategies for human therapeutics. Dr. Doudna will discuss recent findings regarding the molecular mechanism of Cas9 and its use for targeted cell-based therapies.
About the annual Margaret Pittman Lecture:
This annual lecture honors Dr. Margaret Pittman, NIH’s first female lab chief, who made significant contributions to microbiology and vaccine development, particularly in the areas of pertussis and tetanus, during her long career at the National Institute of Allergy and Infectious Diseases.
For more information go to http://wals.od.nih.gov
Author: Jennifer Doudna, Ph.D., Li Ka Shing Chancellor's Chair in Biomedical Sciences and Professor, Department of Molecular and Cell Biology and Department of Chemistry at the University of California, Berkeley; Investigator, Howard Hughes Medical Institute
Permanent link: http://videocast.nih.gov/launch.asp?18890
http://wn.com/CRISPR-Cas_genome_surveillance_From_basic_biology_to_transformative_technology
CRISPR-Cas genome surveillance: From basic biology to transformative technology
Air date: Wednesday, March 11, 2015, 3:00:00 PM
Category: WALS - Wednesday Afternoon Lectures
Runtime: 01:05:13
Description: Wednesday Afternoon Lecture Series
Margaret Pittman Lecture
Dr. Doudna, who specializes in the study of RNA, will present a brief history of the bacterial RNA-guided CRISPR biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism. Using CRISPR-Cas "clustered regularly interspaced short palindromic repeats" technology provides the foundation for remarkable developments in modifying, regulating, or marking genomic loci in a wide variety of cells and organisms. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way to fundamental discoveries in biology with applications in all branches of biotechnology, and strategies for human therapeutics. Dr. Doudna will discuss recent findings regarding the molecular mechanism of Cas9 and its use for targeted cell-based therapies.
About the annual Margaret Pittman Lecture:
This annual lecture honors Dr. Margaret Pittman, NIH’s first female lab chief, who made significant contributions to microbiology and vaccine development, particularly in the areas of pertussis and tetanus, during her long career at the National Institute of Allergy and Infectious Diseases.
For more information go to http://wals.od.nih.gov
Author: Jennifer Doudna, Ph.D., Li Ka Shing Chancellor's Chair in Biomedical Sciences and Professor, Department of Molecular and Cell Biology and Department of Chemistry at the University of California, Berkeley; Investigator, Howard Hughes Medical Institute
Permanent link: http://videocast.nih.gov/launch.asp?18890
- published: 16 Mar 2015
- views: 44
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
- Order: Reorder
- Duration: 2:36
- Updated: 02 Jun 2015
The CRISPR-Cas9 system has revolutionized genetic engineering. This groundbreaking discovery by scientists Jennifer Doudna and Emmanuelle Charpentier has brought medical research light-years closer to finding a cure for many diseases, including sickle cell anemia, HIV, cancer, Huntington's disease and more.
Subscribe to the GE Channel: http://invent.ge/1eGgvZK
GE works on things that matter. The best people and the best technologies taking on the toughest challenges. Finding solutions in energy, health and home, transportation and finance. Building, powering, moving and curing the world. Not just imagining. Doing. GE works.
Connect with GE Online:
Visit GE's Website: http://invent.ge
Find GE on Google +: http://invent.ge/1dPUUN5
Find GE on Tumblr: http://invent.ge/17syCNf
Find GE on Facebook: http://invent.ge/1929rzT
Follow GE on Twitter: http://invent.ge/XJAX15
Follow GE on Pinterest: http://invent.ge/16JjInY
Follow GE on Instagram: http://invent.ge/1a9XZGb
Find GE on LinkedIn: http://invent.ge/1gtUi3e
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
http://wn.com/CRISPR-Cas9_the_Key_to_Fighting_Genetic_Disease?_|_Breaking_Science_|_GE
The CRISPR-Cas9 system has revolutionized genetic engineering. This groundbreaking discovery by scientists Jennifer Doudna and Emmanuelle Charpentier has brought medical research light-years closer to finding a cure for many diseases, including sickle cell anemia, HIV, cancer, Huntington's disease and more.
Subscribe to the GE Channel: http://invent.ge/1eGgvZK
GE works on things that matter. The best people and the best technologies taking on the toughest challenges. Finding solutions in energy, health and home, transportation and finance. Building, powering, moving and curing the world. Not just imagining. Doing. GE works.
Connect with GE Online:
Visit GE's Website: http://invent.ge
Find GE on Google +: http://invent.ge/1dPUUN5
Find GE on Tumblr: http://invent.ge/17syCNf
Find GE on Facebook: http://invent.ge/1929rzT
Follow GE on Twitter: http://invent.ge/XJAX15
Follow GE on Pinterest: http://invent.ge/16JjInY
Follow GE on Instagram: http://invent.ge/1a9XZGb
Find GE on LinkedIn: http://invent.ge/1gtUi3e
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
- published: 02 Jun 2015
- views: 152
WOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New Technology
- Order: Reorder
- Duration: 67:34
- Updated: 10 Feb 2014
CRISPR/Cas-9 is a new genomic editing system that has the potential to revolutionize basic biomedical research and, possibly, the treatment of genetic disease. The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system utilizes guide RNA as a gene-homing mechanism and a Cas-9 nuclease to cut targeted DNA. As such, it drastically reduces the time-to-results and design complexity associated with existing genomic editing tools.
Given CRISPR's simplicity and widespread applicability in biomedical research, scientists and university technology licensing officers are understandably asking about intellectual property rights and access to this technology, both today and looking forward.
This seminar will address the following questions:
What is the CRISPR/Cas-9 system and how does it work as a genome editing tool?
What are CRISPR's advantages over existing genomic editing systems?
What does the IP landscape look like for this technology and what might it look like in the future?
What are the possible impacts of intellectual property on scientists' ability to carry out research using CRISPR/Cas-9?
What are the possible impacts on technology officers' ability to license technology that involves CRISPR/Cas9?
Presenters:
Patricia Granahan, Shareholder, Biotechnology Group, Wolf Greenfield
Chelsea Loughran, Associate, Litigation Group, Wolf Greenfield
http://wn.com/WOLF_GREENFIELD_WEBCAST_CRISPR_Cas-9_and_the_IP_Landscape_for_a_Revolutionary_New_Technology
CRISPR/Cas-9 is a new genomic editing system that has the potential to revolutionize basic biomedical research and, possibly, the treatment of genetic disease. The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system utilizes guide RNA as a gene-homing mechanism and a Cas-9 nuclease to cut targeted DNA. As such, it drastically reduces the time-to-results and design complexity associated with existing genomic editing tools.
Given CRISPR's simplicity and widespread applicability in biomedical research, scientists and university technology licensing officers are understandably asking about intellectual property rights and access to this technology, both today and looking forward.
This seminar will address the following questions:
What is the CRISPR/Cas-9 system and how does it work as a genome editing tool?
What are CRISPR's advantages over existing genomic editing systems?
What does the IP landscape look like for this technology and what might it look like in the future?
What are the possible impacts of intellectual property on scientists' ability to carry out research using CRISPR/Cas-9?
What are the possible impacts on technology officers' ability to license technology that involves CRISPR/Cas9?
Presenters:
Patricia Granahan, Shareholder, Biotechnology Group, Wolf Greenfield
Chelsea Loughran, Associate, Litigation Group, Wolf Greenfield
- published: 10 Feb 2014
- views: 4125
CRISPR technology for genome editing
- Order: Reorder
- Duration: 3:07
- Updated: 29 Jan 2015
CRISPR, a pioneering genome-editing technique, technology will allow AstraZeneca to identify and validate new drug targets in preclinical models that closely resemble human disease.
http://wn.com/CRISPR_technology_for_genome_editing
CRISPR, a pioneering genome-editing technique, technology will allow AstraZeneca to identify and validate new drug targets in preclinical models that closely resemble human disease.
- published: 29 Jan 2015
- views: 7
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
- Order: Reorder
- Duration: 9:41
- Updated: 17 Jul 2015
This is a video protocol for generating genomic deletions in mammalian cells lines using CRISPR/Cas9.
Bauer, D. E., Canver, M. C., Orkin, S. H. Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. J. Vis. Exp. (95), e52118, doi:10.3791/52118 (2015).
http://wn.com/Generation_of_Genomic_Deletions_in_Mammalian_Cell_Lines_via_CRISPR_Cas9
This is a video protocol for generating genomic deletions in mammalian cells lines using CRISPR/Cas9.
Bauer, D. E., Canver, M. C., Orkin, S. H. Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. J. Vis. Exp. (95), e52118, doi:10.3791/52118 (2015).
- published: 17 Jul 2015
- views: 1476
Ethics of CRISPR Gene Editing in Human Stem Cells
- Order: Reorder
- Duration: 22:25
- Updated: 24 Apr 2015
*These are the viewpoints of the authors alone and not the HIA or UMBC.
*We come from a perspective of supporting CRISPR and its use in research.
*This is a an exploratory thinking/sketch argument to start a dialogue. As such it is very simplistic, and we recognize that.
*This is meant for a lay audience mostly, so most of the fine-detailed science and philosophy is glossed over, this is on purpose, but it does not dilute the content.
*There is no "conspiracy to create the movie GATTICA". Many of these techniques are in their infancy, and one should be very careful to not rush to conclusions/judgements. However, it is time to begin a dialogue about these techniques and their purpose.
*One should read this article in order to have a full perspective: http://www.sciencemag.org/content/348/6230/36.summary
*Please comment and make arguments for and against our position below, we welcome alternate viewpoints!
http://wn.com/Ethics_of_CRISPR_Gene_Editing_in_Human_Stem_Cells
*These are the viewpoints of the authors alone and not the HIA or UMBC.
*We come from a perspective of supporting CRISPR and its use in research.
*This is a an exploratory thinking/sketch argument to start a dialogue. As such it is very simplistic, and we recognize that.
*This is meant for a lay audience mostly, so most of the fine-detailed science and philosophy is glossed over, this is on purpose, but it does not dilute the content.
*There is no "conspiracy to create the movie GATTICA". Many of these techniques are in their infancy, and one should be very careful to not rush to conclusions/judgements. However, it is time to begin a dialogue about these techniques and their purpose.
*One should read this article in order to have a full perspective: http://www.sciencemag.org/content/348/6230/36.summary
*Please comment and make arguments for and against our position below, we welcome alternate viewpoints!
- published: 24 Apr 2015
- views: 12
Antibodies Part 1: CRISPR
- Order: Reorder
- Duration: 33:22
- Updated: 12 Jun 2015
In 2012, scientists had a realization: hidden inside one of the world’s smallest organisms, was one of the world’s most powerful tools.
http://wn.com/Antibodies_Part_1_CRISPR
In 2012, scientists had a realization: hidden inside one of the world’s smallest organisms, was one of the world’s most powerful tools.
- published: 12 Jun 2015
- views: 192
LABTalks: CRISPR/Cas9: Simple & versatile genome editing tool
- Order: Reorder
- Duration: 13:45
- Updated: 04 Sep 2014
- author: Life Technologies
Dr. Namritha Ravinder discusses the powerful new CRISPR/ Cas9 technology for genome editing as part of our LABTalks series.
http://wn.com/LABTalks_CRISPR_Cas9_Simple_&_versatile_genome_editing_tool
Dr. Namritha Ravinder discusses the powerful new CRISPR/ Cas9 technology for genome editing as part of our LABTalks series.
- published: 15 Jul 2014
- views: 664
- author: Life Technologies
Feng Zhang Development and Applications of CRISPR Cas9 for Genome Editing
Watch on LabRoots at http://labroots.com/user/webinars/details/id/332 The Cas9 endonuclease from the microbial adaptive immune system CRISPR can be easily pr...
http://wn.com/Feng_Zhang_Development_and_Applications_of_CRISPR_Cas9_for_Genome_Editing
Watch on LabRoots at http://labroots.com/user/webinars/details/id/332 The Cas9 endonuclease from the microbial adaptive immune system CRISPR can be easily pr...
- published: 28 Aug 2014
- views: 24
- author: LabRoots
Rewriting the Genome Using CRISPR and Synthetic Biology
- Order: Reorder
- Duration: 42:58
- Updated: 23 Jan 2015
The CRISPR/Cas9 system has emerged as one of the leading tools for modifying genomes of organisms ranging from E. coli to humans. Additionally, the simple gene targeting mechanism of CRISPR technology has been modified and adapted to other applications that include gene regulation, detection of intercellular trafficking, and pathogen detection. With a wealth of methods for introducing Cas9 and gRNAs into cells, it can be challenging to decide where to start. In this presentation, Dr Adam Clore describes the CRISPR mechanism and some of the most prominent uses for CRISPR, along with methods where IDT technologies can assist scientists in designing, testing, and executing a variety of CRISPR-mediated experiments.
http://wn.com/Rewriting_the_Genome_Using_CRISPR_and_Synthetic_Biology
The CRISPR/Cas9 system has emerged as one of the leading tools for modifying genomes of organisms ranging from E. coli to humans. Additionally, the simple gene targeting mechanism of CRISPR technology has been modified and adapted to other applications that include gene regulation, detection of intercellular trafficking, and pathogen detection. With a wealth of methods for introducing Cas9 and gRNAs into cells, it can be challenging to decide where to start. In this presentation, Dr Adam Clore describes the CRISPR mechanism and some of the most prominent uses for CRISPR, along with methods where IDT technologies can assist scientists in designing, testing, and executing a variety of CRISPR-mediated experiments.
- published: 23 Jan 2015
- views: 68
CSHL Keynote: Dr. Jennifer Doudna, HHMI/University of California, Berkeley
- Order: Reorder
- Duration: 49:15
- Updated: 04 Aug 2014
- author: CSHL Leading Strand
"Programmable RNA-guided genome defense in bacteria" from the 2012 Regulatory & Non-Coding RNAs meeting.
http://wn.com/CSHL_Keynote_Dr._Jennifer_Doudna,_HHMI_University_of_California,_Berkeley
"Programmable RNA-guided genome defense in bacteria" from the 2012 Regulatory & Non-Coding RNAs meeting.
- published: 10 Oct 2013
- views: 1600
- author: CSHL Leading Strand
2) CRISPR Cas9 - Methods and Tools
- Order: Reorder
- Duration: 8:53
- Updated: 06 Feb 2015
In our previous video we introduced the CRISPR/Cas9 system briefly; here we will go over the methods and tools available to you for using the CRISPR/Cas9 system in practice. We invite you to watch our previous video first before continuing with this one: https://www.youtube.com/watch?v=1aJxXWkE3Ek
for more information please visit our website: http://www.abmgood.com/iCRISPR-Cas9-sgRNA.html
http://wn.com/2)_CRISPR_Cas9_-_Methods_and_Tools
In our previous video we introduced the CRISPR/Cas9 system briefly; here we will go over the methods and tools available to you for using the CRISPR/Cas9 system in practice. We invite you to watch our previous video first before continuing with this one: https://www.youtube.com/watch?v=1aJxXWkE3Ek
for more information please visit our website: http://www.abmgood.com/iCRISPR-Cas9-sgRNA.html
- published: 06 Feb 2015
- views: 13
3) CRISPR Cas9 - gRNA Design
- Order: Reorder
- Duration: 7:07
- Updated: 03 Mar 2015
In our previous videos we introduced the CRISPR Cas9 system briefly (http://youtu.be/1aJxXWkE3Ek) and presented the different tools and methods available for their expression in living systems (http://youtu.be/INC_kdr7I34); here we will go over the design criteria of the gRNA and introduce you to the online programs that simplify this procedure. We invite you to watch our previous videos first before continuing with this one.
Chop Chop Harvard: https://chopchop.rc.fas.harvard.edu/
gRNA Design Knowledge Base: Coming Soon!
http://wn.com/3)_CRISPR_Cas9_-_gRNA_Design
In our previous videos we introduced the CRISPR Cas9 system briefly (http://youtu.be/1aJxXWkE3Ek) and presented the different tools and methods available for their expression in living systems (http://youtu.be/INC_kdr7I34); here we will go over the design criteria of the gRNA and introduce you to the online programs that simplify this procedure. We invite you to watch our previous videos first before continuing with this one.
Chop Chop Harvard: https://chopchop.rc.fas.harvard.edu/
gRNA Design Knowledge Base: Coming Soon!
- published: 03 Mar 2015
- views: 25
Webinar: Genome Editing with CRISPR/Cas9
- Order: Reorder
- Duration: 33:36
- Updated: 02 Sep 2014
- author: OriGene Technologies Inc.
OriGene offers CRISPR/Cas9, the latest and effective tool for genome editing. This webinar covers the basics of the CRISPR/Cas9 system, as well as the applic...
http://wn.com/Webinar_Genome_Editing_with_CRISPR_Cas9
OriGene offers CRISPR/Cas9, the latest and effective tool for genome editing. This webinar covers the basics of the CRISPR/Cas9 system, as well as the applic...
- published: 31 Jan 2014
- views: 11536
- author: OriGene Technologies Inc.
- Playlist
- Chat
16:42
Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
http://www.ibiology.org/ibiomagazine/jennifer-doudna-genome-engineering-with-crispr-cas9-b...
published: 23 Mar 2015
Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
http://www.ibiology.org/ibiomagazine/jennifer-doudna-genome-engineering-with-crispr-cas9-birth-of-a-breakthrough-technology.html Talk Overview: Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studies that were previously unachievable. Speaker Biography: Jennifer Doudna is Professor of the Departments of Chemistry and of Molecular and Cell Biology at University of California, Berkeley, and an Investigator of the Howard Hughes Medical Institute. Early in her career, she studied the structure and mechanism of ribozymes (enzymatic RNA molecules) and RNA-protein complexes, such as the signal recognition particle. Now her research focuses on understanding how RNA molecules control gene expression in bacteria, through the CRISPR-Cas9 system, and eukaryotic cells, through RNA interference and microRNA-mediated translational control. For her outstanding scientific contributions, she was elected into the American Academy of Arts and Sciences in 2002 and the National Academy of Sciences in 2003, and was awarded the 2015 Breakthrough Prize in the Life Sciences.- published: 23 Mar 2015
- views: 14
4:13
Genome Editing with CRISPR-Cas9
This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technolo...
published: 05 Nov 2014
Genome Editing with CRISPR-Cas9
Genome Editing with CRISPR-Cas9
This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, including the potential to treat human genetic disease. Feng Zhang, a leader in the development of this technology, is a faculty member at MIT, an investigator at the McGovern Institute for Brain Research, and a core member of the Broad Institute. Further information can be found on Prof. Zhang’s website at http://zlab.mit.edu . Images and footage courtesy of Sputnik Animation, the Broad Institute of MIT and Harvard, Justin Knight and pond5.- published: 05 Nov 2014
- views: 141
4:20
Quick learning of CRISPR/Cas9
What is CRISPR/Cas9? How does CRISPR system work? What is needed to perform genome enginee...
published: 07 May 2014
author: OriGene Technologies Inc.
Quick learning of CRISPR/Cas9
Quick learning of CRISPR/Cas9
What is CRISPR/Cas9? How does CRISPR system work? What is needed to perform genome engineering in your lab? This short video introduces the basics of this no...- published: 07 May 2014
- views: 12575
- author: OriGene Technologies Inc.
5:06
CRISPR Cas9: A novel approach to genetic engineering
Provides the basic mechanism behind the CRISPR Cas9 complex, while describing application ...
published: 23 Apr 2014
author: Walter Wang
CRISPR Cas9: A novel approach to genetic engineering
CRISPR Cas9: A novel approach to genetic engineering
Provides the basic mechanism behind the CRISPR Cas9 complex, while describing application and need.- published: 23 Apr 2014
- views: 7899
- author: Walter Wang
6:22
CRISPR Cas9 - a Brief Introduction
CRISPR-cas9 system is a versatile and robust genome editing system with showing success in...
published: 31 Dec 2014
CRISPR Cas9 - a Brief Introduction
CRISPR Cas9 - a Brief Introduction
CRISPR-cas9 system is a versatile and robust genome editing system with showing success in various model organisms. In this video, you will be introduced to this technology and its various applications and its impact on genome engineering. for more information please visit our website: http://goo.gl/jaKURP- published: 31 Dec 2014
- views: 26
73:19
CRISPR Webinar by Drs. Barrangon, Zhang and Church
Webinar sponsored by OriGene and held by GEN, Genetic Engineering & Biotechnology News Thi...
published: 23 Apr 2014
author: OriGene Technologies Inc.
CRISPR Webinar by Drs. Barrangon, Zhang and Church
CRISPR Webinar by Drs. Barrangon, Zhang and Church
Webinar sponsored by OriGene and held by GEN, Genetic Engineering & Biotechnology News This webinar on the genome editing system CRISPR/Cas9 is given by thre...- published: 23 Apr 2014
- views: 3984
- author: OriGene Technologies Inc.
11:10
The CRISPR-Cas9 System Part 1
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adapt...
published: 01 Mar 2015
The CRISPR-Cas9 System Part 1
The CRISPR-Cas9 System Part 1
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering.- published: 01 Mar 2015
- views: 10
14:33
The CRISPR-Cas9 System Part 2
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adapt...
published: 01 Mar 2015
The CRISPR-Cas9 System Part 2
The CRISPR-Cas9 System Part 2
In this video we discuss the CRISPR-Cas9 system. We first discuss its function as an adaptive immune system against bacteriophages in streptococcus pyogenes. Then we discuss its use in genetic engineering.- published: 01 Mar 2015
- views: 6
1:39
CRISPR Genome Editing - Horizon Discovery
Horizon Discovery GENASSIST™ - CRISPR (clustered regularly interspaced short palindromic r...
published: 08 Apr 2014
author: GENESIS Gene-editing
CRISPR Genome Editing - Horizon Discovery
CRISPR Genome Editing - Horizon Discovery
Horizon Discovery GENASSIST™ - CRISPR (clustered regularly interspaced short palindromic repeats) is a microbial 'immune system' that has evolved to protect ...- published: 08 Apr 2014
- views: 8189
- author: GENESIS Gene-editing
65:13
CRISPR-Cas genome surveillance: From basic biology to transformative technology
CRISPR-Cas genome surveillance: From basic biology to transformative technology
Air date:...
published: 16 Mar 2015
CRISPR-Cas genome surveillance: From basic biology to transformative technology
CRISPR-Cas genome surveillance: From basic biology to transformative technology
CRISPR-Cas genome surveillance: From basic biology to transformative technology Air date: Wednesday, March 11, 2015, 3:00:00 PM Category: WALS - Wednesday Afternoon Lectures Runtime: 01:05:13 Description: Wednesday Afternoon Lecture Series Margaret Pittman Lecture Dr. Doudna, who specializes in the study of RNA, will present a brief history of the bacterial RNA-guided CRISPR biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism. Using CRISPR-Cas "clustered regularly interspaced short palindromic repeats" technology provides the foundation for remarkable developments in modifying, regulating, or marking genomic loci in a wide variety of cells and organisms. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way to fundamental discoveries in biology with applications in all branches of biotechnology, and strategies for human therapeutics. Dr. Doudna will discuss recent findings regarding the molecular mechanism of Cas9 and its use for targeted cell-based therapies. About the annual Margaret Pittman Lecture: This annual lecture honors Dr. Margaret Pittman, NIH’s first female lab chief, who made significant contributions to microbiology and vaccine development, particularly in the areas of pertussis and tetanus, during her long career at the National Institute of Allergy and Infectious Diseases. For more information go to http://wals.od.nih.gov Author: Jennifer Doudna, Ph.D., Li Ka Shing Chancellor's Chair in Biomedical Sciences and Professor, Department of Molecular and Cell Biology and Department of Chemistry at the University of California, Berkeley; Investigator, Howard Hughes Medical Institute Permanent link: http://videocast.nih.gov/launch.asp?18890- published: 16 Mar 2015
- views: 44
2:36
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
The CRISPR-Cas9 system has revolutionized genetic engineering. This groundbreaking discov...
published: 02 Jun 2015
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE
The CRISPR-Cas9 system has revolutionized genetic engineering. This groundbreaking discovery by scientists Jennifer Doudna and Emmanuelle Charpentier has brought medical research light-years closer to finding a cure for many diseases, including sickle cell anemia, HIV, cancer, Huntington's disease and more. Subscribe to the GE Channel: http://invent.ge/1eGgvZK GE works on things that matter. The best people and the best technologies taking on the toughest challenges. Finding solutions in energy, health and home, transportation and finance. Building, powering, moving and curing the world. Not just imagining. Doing. GE works. Connect with GE Online: Visit GE's Website: http://invent.ge Find GE on Google +: http://invent.ge/1dPUUN5 Find GE on Tumblr: http://invent.ge/17syCNf Find GE on Facebook: http://invent.ge/1929rzT Follow GE on Twitter: http://invent.ge/XJAX15 Follow GE on Pinterest: http://invent.ge/16JjInY Follow GE on Instagram: http://invent.ge/1a9XZGb Find GE on LinkedIn: http://invent.ge/1gtUi3e CRISPR-Cas9 the Key to Fighting Genetic Disease? | Breaking Science | GE- published: 02 Jun 2015
- views: 152
67:34
WOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New Technology
CRISPR/Cas-9 is a new genomic editing system that has the potential to revolutionize basic...
published: 10 Feb 2014
WOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New Technology
WOLF GREENFIELD WEBCAST: CRISPR/Cas-9 and the IP Landscape for a Revolutionary New Technology
CRISPR/Cas-9 is a new genomic editing system that has the potential to revolutionize basic biomedical research and, possibly, the treatment of genetic disease. The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system utilizes guide RNA as a gene-homing mechanism and a Cas-9 nuclease to cut targeted DNA. As such, it drastically reduces the time-to-results and design complexity associated with existing genomic editing tools. Given CRISPR's simplicity and widespread applicability in biomedical research, scientists and university technology licensing officers are understandably asking about intellectual property rights and access to this technology, both today and looking forward. This seminar will address the following questions: What is the CRISPR/Cas-9 system and how does it work as a genome editing tool? What are CRISPR's advantages over existing genomic editing systems? What does the IP landscape look like for this technology and what might it look like in the future? What are the possible impacts of intellectual property on scientists' ability to carry out research using CRISPR/Cas-9? What are the possible impacts on technology officers' ability to license technology that involves CRISPR/Cas9? Presenters: Patricia Granahan, Shareholder, Biotechnology Group, Wolf Greenfield Chelsea Loughran, Associate, Litigation Group, Wolf Greenfield- published: 10 Feb 2014
- views: 4125
3:07
CRISPR technology for genome editing
CRISPR, a pioneering genome-editing technique, technology will allow AstraZeneca to identi...
published: 29 Jan 2015
CRISPR technology for genome editing
CRISPR technology for genome editing
CRISPR, a pioneering genome-editing technique, technology will allow AstraZeneca to identify and validate new drug targets in preclinical models that closely resemble human disease.- published: 29 Jan 2015
- views: 7
9:41
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
This is a video protocol for generating genomic deletions in mammalian cells lines using C...
published: 17 Jul 2015
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
This is a video protocol for generating genomic deletions in mammalian cells lines using CRISPR/Cas9. Bauer, D. E., Canver, M. C., Orkin, S. H. Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. J. Vis. Exp. (95), e52118, doi:10.3791/52118 (2015).- published: 17 Jul 2015
- views: 1476
photo: AP / Chris Pizzello/Invision
16 Jul 2015
Vonn was nominated in the Best Comeback Athlete category, which also included New England Patriots’ Rob Gronkowski, New York Yankees’ Alex Rodriguez, and Chicago Bulls’ Derrick Rose, reports the Daily Mail. Lindsey was the only female nominated for Best Comeback Athlete, but Gronkowski won the award. VIDEO. Lindsey Vonn proves she has abs of steel on ‘Conan’ http.//t.co/Ivn9NiHtkr pic.twitter.com/DlMQ3d6csx ... ....
photo: AP / John Bazemore
18 Jul 2015
Chattanooga, Tennessee. A male US Navy petty officer has died of wounds sustained in the shooting at Chattanooga, Tennessee, the US Navy said on Saturday. It takes the number of people killed to five ... The Navy's brief statement did not identify the petty officer who died or provide his age or hometown ... "He was an awesome young man," Darlene Proxmire told Reuters ... He loved the Navy." ... Reuters. ....
photo: AP / Petros Karadjias
18 Jul 2015
Reforms that Greece must implement in return for its latest bailout “will fail” to revive its economy, the country’s former finance minister Yanis Varoufakis has predicted. The package – including VAT rises and pensions cuts – would "go down in history as the greatest disaster of macroeconomic management ever", Mr Varoufakis said ... He said. "This programme is going to fail whoever undertakes its implementation." ... --> ... ....
photo: AP / Shizuo Kambayashi
18 Jul 2015
NICE, France (AP) -- The family of Jules Bianchi says the French Formula One driver has died from head injuries sustained in a crash at last year's Japanese Grand Prix. The news was posted on Bianchi's official Twitter feed early Saturday morning French time and later confirmed by the Manor F1 team. Bianchi, 25, had been in a coma since the Oct ... The pain we feel is immense and indescribable." ... The Manor team tweeted ... ....
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CELLECTA, INC. Receives NIH SBIR Grant to Develop Validated CRISPR Knockout Library of Guide RNA (sgRNA) Sequences
PR Newswire17 Jul 2015
MOUNTAIN VIEW, Calif., July 17, 2015 /PRNewswire/ -- Cellecta, a U.S.-based functional genomics company, announces the receipt of Phase I SBIR grant funds from the National Institute of Health (NIH) to further its CRISPR-mediated genome-editing program ... CRISPR technology can be used to permanently disrupt ("knock out") genes in mammalian cells....
Edit
New Resource Makes Gene Editing Technology Even More User-Friendly (UCSD - University of California - ...
Public Technologies17 Jul 2015
CRISPR/Cas9 is a gene editing tool that can target a particular segment of DNA in living cells and replace it with a new genetic sequence. Researchers at Harvard University and the University of California, San Diego, have developed a new user-friendly resource to accompany the powerful gene editing tool called CRISPR/Cas9, which has been widely adopted to make precise, targeted changes in DNA....
Edit
CELLECTA, INC. Receives NIH SBIR Grant to Develop Validated CRISPR Knockout Library of Guide RNA ...
StreetInsider17 Jul 2015
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The Times of India
16 Jul 2015
The gene-editing technique called CRISPR/Cas9 has been widely adopted to make precise, targeted changes in DNA. The team describes an approach to simplify a labourious part of the gene editing process using the CRISPR/Cas9 system by choosing the best components to match specific gene targets. "We have taken a step towards making the CRISPR/Cas9 ......
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Thoru Pederson to be Next Editor-In-Chief of The FASEB Journal (FASEB - Federation of American Societies for Experimental Biology)
Public Technologies15 Jul 2015
(Source. FASEB - Federation of American Societies for Experimental Biology) http.//www.fasebj.org. Contact. Cody Mooneyhan. Office of Publications. 301-634-7104 . cmooneyhan@faseb.org ... Pederson will be the journal's fourth editor-in-chief since it was established in 1987 ... His laboratory is currently investigating the live cell dynamics of CRISPR gene-editing machinery as well as its potential applications to myotonic dystrophy and ALS ... ###....
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Genome Editing / Genome Engineering Market by Application, Technology & End User - Global Forecast to 2019
PR Newswire15 Jul 2015
NEW YORK, July 15, 2015 /PRNewswire/ -- In this report, the genome editing market is segmented on the basis of applications, technologies, end users, and regions ... The genome editing market, by technology, is categorized into CRISPR, TALEN, ZNF, antisense technology, and other technologies ... The geographic segments included in this report are Asia, Europe, North America, and the Rest of the World (RoW) ... Reasons to Buy the Report. ... US ... Intl....
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UGA researchers develop breakthrough tools in fight against cryptosporidium (The University of Georgia)
Public Technologies15 Jul 2015
(Source. The University of Georgia). Athens, Ga ... Crypto, as researchers often call it, is most commonly spread through tainted drinking or recreational water ... malnourished children and immunocompromised patients ... The team also developed a way to genetically modify the parasite using a technique called CRISPR/Cas9, which allows scientists to make very precise changes to an organism's genome and observe the effects ... distributed by ... (noodl....
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Huffington Post
15 Jul 2015
Could athletic enhancement revolutionize--or undermine--competitive athletics?. It is sport's doomsday scenario. a new generation of bioengineered performance-enhancing agents that can transform also-rans into gold medalists ... "We can't be naive. We have to be realistic ... To be sure, some concerns about genetic enhancement in sports are overblown and, despite dramatic innovations such as CRISPR gene editing, still futuristic ... As former U.S....
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Chinese scientists edit genes to produce artificial sperm capable of creating 'army of half-cloned mice'
South China Morning Post15 Jul 2015
A powerful gene-editing tool called CRISPR-Cas9 has allowed the researchers to add, disrupt or change the sequence of specific genes in the sperm. Using CRISPR-Cas9 together with the man-made sperm, the scientists could now explore the function of many genes simultaneously, while in the past it could only be done one at a time....
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The Irish Times
14 Jul 2015
Gene therapies are advancing much faster than our understanding of these technologies. This was the message from the Conference of the International Society of Computational Biology in Dublin which closes on Tuesday ... The technology was rushing ahead with new techniques for altering genes. One called “CrispR” has the potential to identify and repair defective genes such as those responsible for cystic fibrosis ... ....
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The Los Angeles Times
09 Jul 2015
Researchers are still studying exactly how the microbiome — the billions of bacteria and other tiny critters that inhabit our bodies — impacts human health. . But while they get on with that, engineers are assembling a tool kit that could build designer microbes ... 8 ... 0 ... They used a gene-editing tool known as CRISPR to target two genes that were native to Bacteroides and switch them on and off as well ... --> ... ....
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Yahoo Daily News
08 Jul 2015
When Jennifer Doudna and Emmanuelle Charpentier discovered the most powerful DNA editing technology we know of, CRISPR-Cas9 — something MIT Technology Review described as "the biggest biotech discovery of the century" — they weren't looking for a world-transforming DNA editing tool....
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The Independent
08 Jul 2015
Correcting the genetic defects that cause congenital deafness with gene therapy could be possible within five to ten years according to scientists who claim to have made a breakthrough with the technique ... There are more than 70 different genes that are known to cause deafness when mutated ... Science News in Pictures ... “Our gene therapy protocol is not yet ready for clinical trials ... Scientists call for debate over CRISPR gene therapy ... --> ... ....
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- Lyrics: play full screen play karaoke
- I Don't Think So - Gracia Baur
- This Veil - Cory Sipper
I don't think so
Lights out, bad deal, you don't know how I feel
That's right, get real, don't under estimate me now
Your sick ideals don't have no part in me
Get lost, be gone, you hope that we'll go on
But I don't think so
You want me to be your bitch in bed
But I don't think so
You want me to love you 'til I'm dead
But I don't think so
You want me to make your food, clean up your mess
But I don't think so
This will only happen in your head
Cause I don't think so
Tough luck, you loose, I won't play by your rules
Too much, too soon, don't bother me with your do's and don't's
You're stuck, confused and ancient minded too
You pushed too far, now I got news for you
I don't think so
You want me to be your bitch in bed
But I don't think so
You want me to love you 'til I'm dead
But I don't think so
You want me to make your food, clean up your mess
But I don't think so
This will only happen in your head
Cause I don't think so
You want me to love you 'til I'm dead
This will only happen in your head
W hy do you mess up what we have
I don't think so
You want me to be your bitch in bed
But I don't think so
You want me to love you 'til I'm dead
But I don't think so
You want me to make your food, clean up your mess
But I don't think so
This will only happen in your head
Cause I don't think so
You want me to be your bitch in bed
But I don't think so
You want me to love you 'til I'm dead
But I don't think so
You want me to make your food, clean up your mess
But I don't think so
This will only happen in your head
Cause I don't think so
I froze up when I left myself
you wanna talk about cold?
Have you ever been so chilled your heart
your heart was stuck to your bones
Do you want to know what I hate to feel?
Grab your raincoat, start the engine and drive around the block for anotheryear
Take this Veil
I am through with this hiding out
you can think what you think about me
I've got a lotus in my heart
roots wrap around and connect deep down past the words I form or the things I've
thought
And while we're all gathered round like so,
I'm gonna try and let a bit of that deep down part of me show
Do you want to know what I hate to fear
drop your raincoat, kill the engine and sit around the house for another year
Take this veil...
Oh...I've done it again I lost all my power inside one reaction
Oh...Like radium to lead things change and they will change again
