Oocyte
"Stripped" human oocyte; granulosa cells that had surrounded this oocyte have been removed.
Gray's	subject #3 38
MeSH	Oocytes

An oocyte, ovocyte, or rarely ocyte, is a female gametocyte or germ cell involved in reproduction. In other words, it is an immature ovum, or egg cell. An oocyte is produced in the ovary during female gametogenesis. The female germ cells produce a primordial germ cell (PGC) which undergoes meiosis to form an oogonium. During oogenesis the oogonium becomes a primary oocyte (pronounced oh'a-site).

Formation[link]

Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum.

The formation of an oocyte is called oocytogenesis, which is a part of oogenesis.^[1] Oogenesis results in the formation of both primary oocytes before birth, and of secondary oocytes after it as part of ovulation.

Characteristics[link]

Human oocyte with surrounding granulosa cells

Cytoplasm[link]

Oocytes are rich in cytoplasm which contains yolk granules to nourish the cell early in development.

Nucleus[link]

During the primary oocyte stage of oogenesis, the nucleus is called a germinal vesicle.^[2]

The only normal human type of secondary oocyte has the 23rd (sex) chromosome as 23,X (female-determining), whereas sperm can can have 23,X (female-determining) or 23,Y (male-determining).

Nest[link]

The space wherein an ovum or immature ovum is located is the cell-nest.^[3]

Maternal Contributions to the Oocyte[link]

Oocyte Poles

Because the fate of an oocyte is to become fertilized and ultimately grow into a fully functioning organism, it must be ready to regulate multiple cellular and developmental processes. The oocyte, a large and complex cell, must be supplied with numerous molecules that will direct the growth of the embryo and control cellular activities. As the oocyte is a product of female gametogenesis, the maternal contribution to the oocyte and consequently the newly fertilized egg is enormous. There are many types of molecules that are maternally supplied to the oocyte which will direct various activities within the growing zygote.

Maternal mRNAs and Proteins[link]

During the growth of the oocyte, a variety of maternally transcribed messenger RNAs, or mRNAs, are supplied by maternal cells. These mRNAs can be stored in mRNP (message ribonucleoprotein) complexes and be translated at specific time points, they can be localized within a specific region of the cytoplasm, or they can be homogeneously dispersed within the cytoplasm of the entire oocyte.^[4] Maternally loaded proteins can also be localized or ubiquitous throughout the cytoplasm. The translated products of the mRNAs and the loaded proteins have multiple functions; from regulation of cellular "house-keeping" such as cell cycle progression and cellular metabolism, to regulation of developmental processes such as fertilization, activation of zygotic transcription, and formation of body axes.^[4] Below are some examples of maternally inherited mRNAs and proteins found in Xenopus laevis oocytes.

Maternal Determinants in Xenopus laevis Oocyte

Maternal Mitochondria[link]

The oocyte receives mitochondria from maternal cells, which will go on to control embryonic metabolism and apoptotic events.^[4] The partitioning of mitochondria is carried out by a system of microtubules which will localize mitochondria throughout the oocyte. In certain organisms, such as mammals, paternal mitochondria brought to the oocyte by the spermatozoon are degraded through the attachment of ubiquitinated proteins. The destruction of paternal mitochondria ensures the strictly maternal inheritance of mitochondria and mitochondrial DNA or mtDNA.^[4]

Maternal Nucleolus[link]

In mammals, the nucleolus of the oocyte is derived solely from maternal cells.^[17] The nucleolus, a structure found within the nucleus, is the location where rRNA is transcribed and assembled into ribosomes. While the nucleolus is dense and inactive in a mature oocyte, it is required for proper development of the embryo.^[17]

Maternal Ribosomes[link]

Maternal cells also synthesize and contribute a store of ribosomes that are required for the translation of proteins before the zygotic genome is activated. In mammalian oocytes, maternally derived ribosomes and some mRNAs are stored in a structure called cytoplasmic lattices. These cytoplasmic lattices, a network of fibrils, protein, and RNAs, have been observed to increase in density as the number of ribosomes decrease within a growing oocyte.^[18]

Paternal Contributions to the Oocyte[link]

The spermatozoon which fertilizes an oocyte will contribute its pronucleus, the other half of the zygotic genome. In some species, the spermatozoon will also contribute a centriole which will help make up the zygotic centrosome required for the first division. However, in some species, such as in the mouse, the entire centrosome is acquired maternally.^[19] Currently under investigation is the possibility of other cytoplasmic contributions made to the embryo by the spermatozoon.

Abnormalities[link]

• nondisjunction -- a failure of proper homolog separation in meiosis I, or sister chromatid separation in meiosis II can lead to aneuploidy, in which the oocyte has the wrong number of chromosomes, for example 22,X or 24,X. This is the cause of conditions like Down syndrome and Edwards syndrome. It is more likely with advanced maternal age.
• Some oocytes have multiple nuclei, although it is thought they never mature.

References[link]

Resources[link]

William K. Purves, Gordon H. Orians, David Sadava, H. Craig Heller, Craig Heller (2003). Life: The Science of Biology(7th ed.), pp. 823–824

See also[link]

• Folliculogenesis
• polar body

External links[link]

• Slide