Lipofection

How To Do Transfection, Short Overview Illustrated on Bench Paper

Order: Reorder
Duration: 1:23
Updated: 12 Aug 2013

This short transfection overview explains the basics of transfection and will help you gain a better understanding of how it works and how to do it. Check ou...

http://wn.com/How_To_Do_Transfection,_Short_Overview_Illustrated_on_Bench_Paper

Plasmid Transfection Protocol

Order: Reorder
Duration: 3:38
Updated: 13 Aug 2013

Learn more at http://www.lifetechnologies.com/transfection Optimized protocol for Lipofectamine LTX & Plus reagent: http://tools.invitrogen.com/content/sfs/m...

http://wn.com/Plasmid_Transfection_Protocol

Lipofection - Spoken Description

Order: Reorder
Duration: 1:03
Updated: 19 Feb 2014

Spoken description about Lipofection. This is text to speech video for education the public. Below is the transcript for the recording: Lipofection is a technique used to inject genetic material into a cell by means of liposomes, which are vesicles that can easily merge with the cell membrane since they are both made of a phospholipid bilayer. Lipofection generally uses a positively charged lipid to form an aggregate with the negatively charged genetic material. A net positive charge on this aggregrate has been assumed to increase the effectiveness of transfection through the negatively charged phospholipid bilayer. This transfection technology performs the same tasks as other biochemical procedures utilizing polymers, DEAE dextran, calcium phosphate, and electroporation. The main advantages of lipofection are its high efficiency, its ability to transfect all types of nucleic acids in a wide range of cell types, its ease of use, reproducibility, and low toxicity. In addition, this method is suitable for all transfection applications . High throughput screening assay has also shown good efficiency in some in vivo models. Copyright (c) 2014 Spoken Word Edu Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.3 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation License". Content in this site is licensed under a Creative Commons Lisence (http://creativecommons.org/licenses/by-sa/3.0/). Copyright 2014 Spoken Word Edu

http://wn.com/Lipofection_Spoken_Description

TRANSFECTION

Order: Reorder
Duration: 5:42
Updated: 26 Sep 2012

Nolan explains how to do a transfection with 293T cells using Lipofectamine 2000 reagent from Invitrogen.

http://wn.com/TRANSFECTION

Archaeogastropoda - Spoken Description

Order: Reorder
Duration: 1:21
Updated: 19 Feb 2014

Spoken description about Archaeogastropoda. This is text to speech video for education the public. Below is the transcript for the recording: Archaeogastropoda was a taxonomic order of sea snails used in older classifications of gastropods, i.e. snails and slugs. Archeogastropoda are marine prosobranch gastropod mollusks, mainly herbivores, typically having two gills and a double-chambered heart, with the eggs and sperm discharged directly into the water. They were traditionally regarded as a relatively primitive group. This older classification of the gastropods is based on the classification of Johannes Thiele . This classification was not based on true phylogenetic relationships, but on more general affinities between the groups. In the last few years, two new cladistic taxonomies of the gastropods have been published . This has led to an extensive reclassification of gastropod taxa. The taxon Archaeogastropoda was found to be a paraphyletic group, and therefore unacceptable in a strictly cladistic classification. In the 1997 classification, most of the former Archaeogastropoda were included in: subclass Eogastropoda *order Patellogastropoda subclass Orthogastropoda *Superorder Vetigastropoda Salvini-Plawen, 1989 *Superorder Neritaemorphi Koken, 1896 . A more detailed classification can be found on Gastropoda. Copyright (c) 2014 Spoken Word Edu Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.3 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation License". Content in this site is licensed under a Creative Commons Lisence (http://creativecommons.org/licenses/by-sa/3.0/). Copyright 2014 Spoken Word Edu

http://wn.com/Archaeogastropoda_Spoken_Description

Genotoxicity - Spoken Description

Order: Reorder
Duration: 1:09
Updated: 19 Feb 2014

Spoken description about Genotoxicity. This is text to speech video for education the public. Below is the transcript for the recording: In genetics, genotoxicity describes a deleterious action on a cell's genetic material affecting its integrity. This includes both certain chemical compounds and certain types of radiation. Genotoxic substances are all those with affinity to interact with DNA - which is not proof of their dangerousness to humans, but does render them potentially mutagenic or carcinogenic. Typical genotoxins like aromatic amines are believed to cause mutations because they are nucleophilic and form strong covalent bonds with DNA resulting with the formation of aromatic amine-DNA adducts, preventing accurate replication. Genotoxins affecting sperm and eggs can pass genetic changes down to descendants who have never been exposed to the genotoxin. The term "genotoxic" started being more commonly used after the publication by Lars Ehrenberg et al., in 1973, of "The relation of cancer induction and genetic damage. In: Evaluation of Genetic Risks of Environmental Chemicals. Royal Swedish Academy of Sciences" - on the relation between genetic damage and cancer. Copyright (c) 2014 Spoken Word Edu Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.3 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation License". Content in this site is licensed under a Creative Commons Lisence (http://creativecommons.org/licenses/by-sa/3.0/). Copyright 2014 Spoken Word Edu

http://wn.com/Genotoxicity_Spoken_Description

QVprep Genetic Engineering App - Learn, Review and Test in Genetics

Order: Reorder
Duration: 0:37
Updated: 01 Sep 2013

Covers * Introduction & History of Genetic Engineering * Genetic Engineering Techniques * Genetic Engineering Experiments * Molecular Cloning * Gene Targeting & DNA Insertion Techniques * Genetically Modified Organisms * Genetically Modified Crops * Genetically Modified Food * Gene Therapy * Human Genome Project * Recombinant DNA Technology * Genome Engineering * Plant Genetic Engineering * Genetic Screening for Human Diseases * Synthetic Biology QVprep Genetic Engineering is ideal for * College majors in Genetics * Undergraduates doing Genetic Engineering * Junior Physicians * Engineering students * Medical, Pre-medical and Nursing students * Medical technicians, Laboratory technicians and Physician assistants * Preparing for job interviews * Hospitals and labs to provide training to their staff * Even 11th and 12th graders Features: A) LEARN MODE The app allows you to learn Genetic Engineering concepts in a very simple and user friendly manner. In the "Learn" mode of the app, you click on the "Concepts" button, review the "concept text" and then answer multiple choice questions related to the concept that you just reviewed. If you answer wrong, a detailed "answer explanation" will be displayed, which will reinforce learning. Proceed in this manner to complete a particular topic at your own pace. Thus, by referring to the "Genetic Engineering Concept text" and then answering multiple choice questions, your learning is faster and complete. B) TEST MODE In the "Test" mode, the app does not show the context text to help you. Instead, you use your learning from the app and attempt multiple choice questions. Again, the app prompts you with detailed answer explanation in case you answer wrong. This proven process again reinforces learning. Share your test score by emailing the scores to yourself or someone else. The app also allows you to post your score on your Facebook page or your Twitter account. With an exhaustive content on Genetics and Genetic Engineering, we are sure you will be able to learn, review and excel in this subject. QVprep Genetic Engineering is useful for * Exam preparation in genetics and genetic engineering * Revision of concepts to aid in your career in engineering & medicine * Genetics Concept Review Learn and take tests on these topics and concepts Introduction & History of Genetic Engineering: Milestones, Experiments, Progress, Applications, Concerns, Opposition & Regulations Genetic Engineering Techniques: Gel Electrophoresis, Restriction digestion & ligation, Polymerase Chain Reaction, Recombinant DNA technology, Gene Targeting, DNA Sequencing, Vectors & selectable markers, Western blot, Southern Hybridization, Transformation, DNA Fingerprinting Genetic Engineering Experiments: Animal cloning, Stem cell research, Tissue culture, Knockout mice, Recombinant protein production, Terminator technology, Zebra fish studies, Pharming Molecular Cloning: Cloning history, Steps in molecular cloning, Uses of cloning, Opposition to cloning Gene Targeting and DNA Insertion Techniques: Microinjection, Lipofection, Electroporation, Transfection, Agrobacterium-induced DNA transfer, Gene gun Genetically Modified Organisms: Transgenic plants, crops, animals, microbes, insects & transgenic fish Genetically Modified Crops: History, Methods, examples and uses of genetically modified crops Genetically Modified Food: Basics, History, Production & Testing of genetically modified food Gene Therapy: Types, Vectors used, Milestones & Issues with gene therapy Human Genome Project: Introduction, History, Benefits and Issues with HGP Recombinant DNA Technology: Creating Recombinant DNA & applications of recombinant DNA technology Genome Engineering: Basics & methods of genome engineering, MAGE Plant Genetic Engineering: Plant tissue culture, Micropropagation, Protoplast fusion & Applications of plant tissue culture Genetic Screening for Human Diseases: Advanced genetic screening, Positional cloning, types of Genetic testing Synthetic Biology: Historical milestones & Uses of synthetic biology, Techniques used & Examples of synthetic biology research PJP Consulting LLC * Our mission is to develop educational apps for every subject and every school and college so that today's modern technology like smart phones and tablets is used for education and learning of school, college and academic curriculum. http://www.Genetics.Qvprep.com

http://wn.com/QVprep_Genetic_Engineering_App_Learn,_Review_and_Test_in_Genetics

Allelopathy - Spoken Description

Order: Reorder
Duration: 1:17
Updated: 19 Feb 2014

Spoken description about Allelopathy. This is text to speech video for education the public. Below is the transcript for the recording: Allelopathy is a biological phenomenon by which an organism produces one or more biochemicals that influence the growth, survival, and reproduction of other organisms. These biochemicals are known as allelochemicals and can have beneficial or detrimental effects on the target organisms. Allelochemicals are a subset of secondary metabolites, which are not required for metabolism of the allelopathic organism. Allelochemicals with negative allelopathic effects are an important part of plant defense against herbivory. Allelopathy is characteristic of certain plants, algae, bacteria, coral, and fungi. Allelopathic interactions are an important factor in determining species distribution and abundance within plant communities, and are also thought to be important in the success of many invasive plants. For specific examples, see Spotted Knapweed , Garlic Mustard , and Nutsedge. The process by which a plant acquires more of the available resources from the environment without any chemical action on the surrounding plants is called resource competition. This process is not negative allelopathy, although both processes can act together to enhance the survival rate of the plant species. Copyright (c) 2014 Spoken Word Edu Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.3 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation License". Content in this site is licensed under a Creative Commons Lisence (http://creativecommons.org/licenses/by-sa/3.0/). Copyright 2014 Spoken Word Edu

http://wn.com/Allelopathy_Spoken_Description

International Ornithological Committee - Spoken Description

Order: Reorder
Duration: 0:15
Updated: 20 Feb 2014

Spoken description about International Ornithological Committee. This is text to speech video for education the public. Below is the transcript for the recording: The International Ornithological Committee is a group of about 200 international ornithologists, and is responsible for the International Ornithological Congress and other international ornithological activities, undertaken by its Standing Committees. Copyright (c) 2014 Spoken Word Edu Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.3 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation License". Content in this site is licensed under a Creative Commons Lisence (http://creativecommons.org/licenses/by-sa/3.0/). Copyright 2014 Spoken Word Edu

http://wn.com/International_Ornithological_Committee_Spoken_Description

What does NETpositive mean to you?

Order: Reorder
Duration: 1:50
Updated: 08 Aug 2012

http://wn.com/What_does_NETpositive_mean_to_you?

Alexia's cooperation speech

Order: Reorder
Duration: 2:41
Updated: 24 Oct 2013

http://wn.com/Alexia's_cooperation_speech

Membrane transport - Spoken Description

Order: Reorder
Duration: 1:22
Updated: 19 Feb 2014

Spoken description about Membrane transport. This is text to speech video for education the public. Below is the transcript for the recording: In cellular biology the term membrane transport refers to the collection of mechanisms that regulate the passage of solutes such as ions and small molecules through biological membranes, which are lipid bilayers that contain proteins embedded in them. The regulation of passage through the membrane is due to selective membrane permeability - a characteristic of biological membranes which allows them to separate substances of distinct chemical nature. In other words, they can be permeable to certain substances but not to others. The movements of most solutes through the membrane are mediated by membrane transport proteins which are specialized to varying degrees in the transport of specific molecules. As the diversity and physiology of the distinct cells is highly related to their capacities to attract different external elements, it is postulated that there is a group of specific transport proteins for each cell type and for every specific physiological stage. This differential expression is regulated through the differential transcription of the genes coding for these proteins and its translation, for instance, through genetic-molecular mechanisms, but also at the cell biology level: the production of these proteins can be activated by cellular signaling pathways, at the biochemical level, or even by being situated in cytoplasmic vesicles. Copyright (c) 2014 Spoken Word Edu Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.3 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation License". Content in this site is licensed under a Creative Commons Lisence (http://creativecommons.org/licenses/by-sa/3.0/). Copyright 2014 Spoken Word Edu

http://wn.com/Membrane_transport_Spoken_Description

Genetic Transfer - part 1

Order: Reorder
Duration: 6:41
Updated: 26 Jul 2013

Microbial populations achieve genetic diversity through horizontal gene transfer. Bacteria may transfer genes from one to another by conjugation, transformat...

http://wn.com/Genetic_Transfer_part_1

siRNA Transfection Protocol

Order: Reorder
Duration: 4:00
Updated: 01 Aug 2013

Learn more at http://www.lifetechnologies.com/transfection How to perform siRNA transfection with Lipofectamine® RNAiMAX protocol. Superior siRNA/miRNA deliv...

http://wn.com/siRNA_Transfection_Protocol

Mod-01 Lec-04 Proximal & Distal Promoter Elements,Enhancers and Silencers, Gene-specific Regulators

Order: Reorder
Duration: 59:02
Updated: 09 Apr 2013

Eukaryotic Gene Expression:Basics & Benefits by Prof.P N RANGARAJAN,Department of Biochemistry,IISC Bangalore. For more details on NPTEL visit http://nptel.i...

http://wn.com/Mod-01_Lec-04_Proximal_Distal_Promoter_Elements,Enhancers_and_Silencers,_Gene-specific_Regulators

How To Do Transfection, Short Overview Illustrated on Bench Paper

Plasmid Transfection Protocol

Lipofection - Spoken Description

TRANSFECTION

Archaeogastropoda - Spoken Description

Genotoxicity - Spoken Description

QVprep Genetic Engineering App - Learn, Review and Test in Genetics

Allelopathy - Spoken Description

International Ornithological Committee - Spoken Description

What does NETpositive mean to you?

Alexia's cooperation speech

Membrane transport - Spoken Description

Genetic Transfer - part 1

siRNA Transfection Protocol

Make changes yourself !

How To Do Transfection, Short Overview Illustrated on Bench Paper

Plasmid Transfection Protocol

Lipofection - Spoken Description

TRANSFECTION

Archaeogastropoda - Spoken Description

Genotoxicity - Spoken Description

QVprep Genetic Engineering App - Learn, Review and Test in Genetics

Allelopathy - Spoken Description

International Ornithological Committee - Spoken Description

What does NETpositive mean to you?

Alexia's cooperation speech

Membrane transport - Spoken Description

Genetic Transfer - part 1

siRNA Transfection Protocol

Mod-01 Lec-04 Proximal & Distal Promoter Elements,Enhancers and Silencers, Gene-specific Regulators