Jacqueline of Wittelsbach (Dutch: Jacoba van Beieren; French: Jacqueline de Bavière) (16 August 1401 – 8 October 1436) was Duchess of Bavaria-Straubing, Countess of Hainaut and Holland from 1417 to 1432. She was also Dauphine of France for a period of less than a year and a half between 1415 and 1417 and Duchess of Gloucester for a period during the 1420s, if her marriage to Humphrey, Duke of Gloucester, is accepted as valid.

Jacqueline was the last Wittelsbach ruler of Hainaut and Holland. Following her death, her estates passed into the inheritance of Philip the Good, Duke of Burgundy.

Born in the Castle of Le Quesnoy in Hainaut, Jacqueline, from her birth, was referred to as "of Holland", indicating that she was the heiress of her father's estates. She was the only daughter of William II, Duke of Bavaria (also known as William VI, Count of Holland) from his marriage with Margaret of Burgundy, daughter of Margaret III of Flanders and Philip the Bold. She was born after 16 years of an otherwise childless marriage. At the age of only twenty-two months (in Paris on 5 May 1403) and again at the age of four (in Compiègne on 29 June 1406), Jacqueline was betrothed to John, Duke of Touraine, fourth son of King Charles VI of France and Queen Isabeau of Bavaria. Both children were brought up in the Castle of Le Quesnoy, Jacqueline's birth place. The boy had been given into tutelage of his father-in-law, since he was expected to succeed as ruler in Hainaut and not in any way in France itself. It was a happy youth with both children given a very good education. On 22 April 1411 the Pope gave his dispensation for the union and on 6 August 1415, when Jacqueline was just fourteen, she and John married in The Hague.