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Human embryonic stem cells in cell culture

Pluripotent: Embryonic stem cells are able to develop into any type of cell, excepting those of the placenta. Only embryonic stem cells of the morula are totipotent: able to develop into any type of cell, including those of the placenta.

Embryonic stem cells (ES cells) are pluripotent stem cells derived from the inner cell mass of the blastocyst, an early-stage embryo.^[1] Human embryos reach the blastocyst stage 4–5 days post fertilization, at which time they consist of 50–150 cells. Isolating the embryoblast or inner cell mass (ICM) results in destruction of the fertilized human embryo, which raises ethical issues. Those issues include whether or not a human life at the embryonic stage should be granted the moral status of a human being as it is with a child or an adult.^{[2] [3]}

Types[link]

Embryonic stem cells are distinguished by two distinctive properties:

• their pluripotency, and
• their ability to replicate indefinitely.^[4]

ES cells are pluripotent, that is, they are able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. These include each of the more than 220 cell types in the adult body. Pluripotency distinguishes embryonic stem cells from adult stem cells found in adults; while embryonic stem cells can generate all cell types in the body, adult stem cells are multipotent and can produce only a limited number of cell types. Human ES cells measure approximately 14μm while mouse ES cells are closer to 8μm.^[5]

Additionally, under defined conditions, embryonic stem cells are capable of propagating themselves indefinitely. This allows embryonic stem cells to be employed as useful tools for both research and regenerative medicine, because they can produce limitless numbers of themselves for continued research or clinical use.

Because of their plasticity and potentially unlimited capacity for self-renewal, ES cell therapies have been proposed for regenerative medicine and tissue replacement after injury or disease. Diseases that could potentially be treated by pluripotent stem cells include a number of blood and immune-system related genetic diseases, cancers, and disorders; juvenile diabetes; Parkinson's; blindness and spinal cord injuries. Besides the ethical concerns of stem cell therapy (see stem cell controversy), there is a technical problem of graft-versus-host disease associated with allogeneic stem cell transplantation. However, these problems associated with histocompatibility may be solved using autologous donor adult stem cells, therapeutic cloning, stem cell banks or more recently by reprogramming of somatic cells with defined factors (e.g. induced pluripotent stem cells). Other potential uses of embryonic stem cells include investigation of early human development, study of genetic disease and as in vitro systems for toxicology testing.

Research history and developments[link]

In 1964, researchers isolated a single type of cell from a teratocarcinoma, a tumor now known to be derived from a germ cell. These cells isolated from the teratocarcinoma replicated and grew in cell culture as a stem cell and are now known as embryonic carcinoma (EC) cells.^[6] Although similarities in morphology and differentiating potential (pluripotency) led to the use of EC cells as the in vitro model for early mouse development,^[7] EC cells harbor genetic mutations and often abnormal karyotypes that accumulated during the development of the teratocarcinoma. These genetic aberrations further emphasized the need to be able to culture pluripotent cells directly from the inner cell mass.

Martin Evans

In 1981, embryonic stem cells (ES cells) were independently first derived from mouse embryos by two groups. Martin Evans and Matthew Kaufman from the Department of Genetics, University of Cambridge published first in July, revealing a new technique for culturing the mouse embryos in the uterus to allow for an increase in cell number, allowing for the derivation of ES cells from these embryos.^[8] Gail R. Martin, from the Department of Anatomy, University of California, San Francisco, published her paper in December and coined the term “Embryonic Stem Cell”.^[9] She showed that embryos could be cultured in vitro and that ES cells could be derived from these embryos. In 1998, a breakthrough occurred when researchers, led by James Thomson at the University of Wisconsin-Madison, first developed a technique to isolate and grow human embryonic stem cells in cell culture.^[10]

Techniques and Conditions for Embryonic Stem Cell Derivation and Culture[link]

Derivation of Human Embryonic Stem Cells:
In vitro fertilization generates multiple embryos. The surplus of embryos is not clinically used or is unsuitable for implantation into the patient, and therefore may be donated by the donor with consent. Human embryonic stem cells are derived from these donated embryos that would otherwise be discarded.^[11] The inner cell mass (cells of interest), from the blastocyst stage of the embryo, is separated from the trophectoderm, the cells that would differentiate into extra-embryonic tissue. Immunosurgery, the process in which antibodies are bound to the trophectoderm and removed by another solution, and mechanical dissection are performed to achieve separation. The resulting inner cell mass cells are plated onto cells that will supply support. The inner cell mass cells attach and expand further to form a human embryonic cell line, which are undifferentiated. These cells are fed daily and are enzymatically or mechanically separated every four to seven days. For differentiation to occur, the human embryonic stem cell line is removed from the supporting cells to form embryoid bodies, is co-cultured with a serum containing necessary signals, or is grafted in a three-dimensional scaffold to result.^[12]

Derivation of Embryonic Stem Cells from other animals:
Embryonic stem cells are derived from the inner cell mass of the early embryo, which are harvested from the donor mother animal. Martin Evans and Matthew Kaufman reported a technique that delays embryo implantation, allowing the inner cell mass to increase. This process includes removing the donor mother’s ovaries and dosing her with progesterone, changing the hormone environment, which causes the embryos to remain free in the uterus. After 4–6 days of this intrauterine culture, the embryos are harvested and grown in in vitro culture until the inner cell mass forms “egg cylinder-like structures,” which are dissociated into single cells, and plated on fibroblasts treated with mitomycin-c (to prevent fibroblast mitosis). Clonal cell lines are created by growing up a single cell. Evans and Kaufman showed that the cells grown out from these cultures could form teratomas and embryoid bodies, and differentiate in vitro, all of which indicating that the cells are pluripotent.^[8]

Gail Martin derived and cultured her ES cells differently. She removed the embryos from the donor mother at approximately 76 hours after copulation and cultured them overnight in media containing serum. The following day, she removed the inner cell mass from the late blastocyst using microsurgery. The extracted inner cell mass was cultured on fibroblasts treated with mitomycin-c in media that containing serum and was conditioned by EC cells. After approximately one week, colonies of cells grew out. These cells grew in culture and demonstrated pluripotent characteristics, as demonstrated by the ability to form teratomas, differentiate in vitro, and form embryoid bodies. Martin referred to these cells as ES cells.^[9]

It is now known that the feeder cells provide leukemic inhibitory factor (LIF) and serum provides bone morphogenetic proteins (BMPs) that are necessary to prevent ES cells from differentiating.^[13][14] These factors are extremely important for the efficiency of deriving ES cells. Furthermore, it has been demonstrated that different mouse strains have different efficiencies for isolating ES cells.^[15] Current uses for mouse ES cells include the generation of transgenic mice, including knockout mice. For human treatment, there is a need for patient specific pluripotent cells. Generation of human ES cells is more difficult and faces ethical issues. So, in addition to human ES cell research, many groups are focused on the generation of induced pluripotent stem cells (iPS cells).^[16]

Contamination by reagents used in cell culture[link]

The online edition of Nature Medicine published a study on January 24, 2005, which stated that the human embryonic stem cells available for federally funded research are contaminated with non-human molecules from the culture medium used to grow the cells.^[17] It is a common technique to use mouse cells and other animal cells to maintain the pluripotency of actively dividing stem cells. The problem was discovered when non-human sialic acid in the growth media was found to compromise the potential uses of the embryonic stem cells in humans, according to scientists at the University of California, San Diego.^[18]

However, a study published in the online edition of Lancet Medical Journal on March 8, 2005 detailed information about a new stem cell line that was derived from human embryos under completely cell- and serum-free conditions. After more than 6 months of undifferentiated proliferation, these cells demonstrated the potential to form derivatives of all three embryonic germ layers both in vitro and in teratomas. These properties were also successfully maintained (for more than 30 passages) with the established stem cell lines.^[19]

Potential Clinical Use[link]

Human embryonic stem cells have the ability to differentiate into various cell types, therefore useful for transplantation or tissue engineering(Langer, Robert).

Embryoid bodies 24 hours after formation.

Reducing donor-host rejection[link]

There is also ongoing research to reduce the potential for rejection of the differentiated cells derived from ES cells once researchers are capable of creating an approved therapy from ES cell research. One of the possibilities to prevent rejection is by creating embryonic stem cells that are genetically identical to the patient via therapeutic cloning.

An alternative solution for rejection by the patient to therapies derived from non-cloned ES cells is to derive many well-characterized ES cell lines from different genetic backgrounds and use the cell line that is most similar to the patient; treatment can then be tailored to the patient, minimizing the risk of rejection.

Safety: reducing the risk of teratoma and other cancers as a side effect[link]

• The major concern with the possible transplantation of ESC into patients as therapies is their ability to form tumors including teratoma.^[20] Safety issues prompted the FDA to place a hold on the first ESC clinical trial (see below), however no tumors were observed.

• The main strategy to enhance the safety of ESC for potential clinical use is to differentiate the ESC into specific cell types (e.g. neurons, muscle, liver cells) that have reduced or eliminated ability to cause tumors. Following differentiation, the cells are subjected to sorting by flow cytometry for further purification. ESC are predicted to be inherently safer than IPS cells because they are not genetically modified with genes such as c-Myc that are linked to cancer. Nonetheless, ESC express very high levels of the iPS inducing genes and these genes including Myc are essential for ESC self-renewal and pluripotency,^[21] and potential strategies to improve safety by eliminating Myc expression are unlikely to preserve the cells' "stemness".

First clinical trial[link]

On January 23, 2009, Phase I clinical trials for transplantation of oligodendrocytes (a cell type of the brain and spinal cord) derived from human ES cells into spinal cord-injured individuals received approval from the U.S. Food and Drug Administration (FDA), marking it the world's first human ES cell human trial.^[22] The study leading to this scientific advancement was conducted by Hans Keirstead and colleagues at the University of California, Irvine and supported by Geron Corporation of Menlo Park, CA. A previous experiment had shown an improvement in locomotor recovery in spinal cord-injured rats after a 7-day delayed transplantation of human ES cells that had been pushed into an oligodendrocytic lineage.^[23] In the proposed phase I clinical study, about eight to ten paraplegics who have had their injuries no longer than two weeks before the trial begins, will be selected, since the cells must be injected before scar tissue is able to form. However, the researchers are emphasizing that the injections are not expected to fully cure the patients and restore all mobility. Based on the results of the rodent trials, researchers say restoration of myelin sheathes, and an increase in mobility is probable. This first trial is mainly testing the safety of these procedures and if everything goes well, it could lead to future studies that involve people with more severe disabilities.^[24] The trial had been put on hold in August 2009 due to concerns made by the FDA regarding a small number of microscopic cysts found in several treated rat models but the hold has been lifted as of July 30, 2010.^[25][26][27]

In October 2010 researchers enrolled and administered ESTs to the first patient at Shepherd Center in Atlanta.^[28] The makers of the stem cell therapy, Geron Corporation, estimate that it will take several months for the stem cells to replicate and for the GRNOPC1 therapy to be evaluated for success or failure. In November 2011 Geron announced it was dropping out of stem cell research for financial reasons, but would continue to monitor existing patients, and was attempting to find a partner that could continue their research.^[29]

Potential method for new cell line derivation[link]

On August 23, 2006, the online edition of Nature scientific journal published a letter by Dr. Robert Lanza (medical director of Advanced Cell Technology in Worcester, MA) stating that his team had found a way to extract embryonic stem cells without destroying the actual embryo.^[30] This technical achievement would potentially enable scientists to work with new lines of embryonic stem cells derived using public funding in the USA, where federal funding was at the time limited to research using embryonic stem cell lines derived prior to August 2001. In March, 2009, the limitation was lifted.^[31]

Recently, it was shown that pluripotent stem cells highly similar to embryonic stem cells can be generated by the delivery of three genes (Oct4, Sox2, and Klf4) to differentiated cells.^[32] The delivery of these genes "reprograms" differentiated cells into pluripotent stem cells, allowing for the generation of pluripotent stem cells without the embryo. Because ethical concerns regarding embryonic stem cells typically are about their derivation from terminated embryos, it is believed that reprogramming to these "induced pluripotent stem cells" (iPS cells) may be less controversial. Both human and mouse cells can be reprogrammed by this methodology, generating both human pluripotent stem cells and mouse pluripotent stem cells without an embryo.^[33]

This may enable the generation of patient specific ES cell lines that could potentially be used for cell replacement therapies. In addition, this will allow the generation of ES cell lines from patients with a variety of genetic diseases and will provide invaluable models to study those diseases.

However, as a first indication that the induced pluripotent stem cell (iPS) cell technology can in rapid succession lead to new cures, it was used by a research team headed by Rudolf Jaenisch of the Whitehead Institute for Biomedical Research in Cambridge, Massachusetts, to cure mice of sickle cell anemia, as reported by Science journal's online edition on December 6, 2007.^[34]

On January 16, 2008, a California based company, Stemagen, announced that they had created the first mature cloned human embryos from single skin cells taken from adults. These embryos can be harvested for patient matching embryonic stem cells.^[35]

Use of human embryonic stem cells as models for human genetic disorders[link]

Several new studies have started to address this issue. This has been done either by genetically manipulating the cells, or more recently by deriving diseased cell lines identified by prenatal genetic diagnosis (PGD). This approach may very well prove invaluable at studying disorders such as Fragile-X syndrome, Cystic fibrosis, and other genetic maladies that have no reliable model system.

Yury Verlinsky (Sept, 1, 1943 – July 16, 2009), a Russian-American medical researcher who specialized in embryo and cellular genetics (genetic cytology), developed prenatal diagnosis testing methods to determine genetic and chromosomal disorders a month and a half earlier than standard amniocentesis. The techniques are now used by many pregnant women and prospective parents, especially those couples with a history of genetic abnormalities or where the woman is over the age of 35, when the risk of genetically-related disorders is higher. In addition, by allowing parents to select an embryo without genetic disorders, they have the potential of saving the lives of siblings that already had similar disorders and diseases using cells from the disease free offspring.^[36]

See also[link]

• Embryonic Stem Cell Research Oversight (ESCRO) Committees
• Embryoid body
• Stem cell controversy
• Fetal tissue implant

References[link]

Langer, Robert. "Endothelial Cells Derived from Human Embryonic Stem Cells." Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences, 26 Mar. 2002. Web. 15 Apr. 2012. <http://www.pnas.org/content/99/7/4391.short>.

External links[link]

• Understanding Stem Cells: A View of the Science and Issues from the National Academies
• National Institutes of Health
• Fact sheet on embryonic stem cells
• Fact sheet on ethical issues in embryonic stem cell research
• The Center for Bioethics & Human Dignity
• Information & Alternatives to Embryonic Stem Cell Research
• A blog focusing specifically on ES cells and iPS cells including research, biotech, and patient-oriented issues

• Rappaport Institute of the Technion [1]
• insertech.simdif.com

Shinya Yamanaka
Born	(1962-09-04) September 4, 1962 (age 49) Higashiōsaka, Osaka, Japan
Nationality	Japanese
Fields	stem cell research[1][2][3]
Institutions	Kyoto University, Gladstone Institute of Cardiovascular Disease
Alma mater	Kobe University Osaka City University Gladstone Institute of Cardiovascular Disease
Known for	Induced Pluripotent Stem Cell
Notable awards	Robert Koch Prize (2008) Shaw Prize (2008) Gairdner Foundation International Award (2009) Albert Lasker Basic Medical Research Award (2009) BBVA Foundation Frontiers of Knowledge Award (2010) Wolf Prize (2011) Fellow of the National Academy of Sciences[4] (2012)

Shinya Yamanaka (山中 伸弥, Yamanaka Shin'ya^?, born September 4, 1962 in Higashiōsaka) is a Japanese physician and adult stem cell researcher.^[1][2][3] He serves as the director of Center for iPS Cell Research and Application and a professor at the Institute for Frontier Medical Sciences at Kyoto University, as a senior investigator at the UCSF-affiliated J. David Gladstone Institutes in San Francisco, California, and as a professor of anatomy at University of California, San Francisco (UCSF). He was recently awarded the Wolf Prize in Medicine. Yamanaka has been listed as one of the 15 Asian Scientists To Watch by Asian Scientist Magazine on 15 May 2011.^[5][6]

Biography[link]

He received his M.D. at Kobe University in 1987 and his Ph.D. at Osaka City University Graduate School in 1993. After this he went through a residency in orthopedic surgery at National Osaka Hospital and a postdoctoral fellowship at the Gladstone Institute of Cardiovascular Disease, San Francisco.

Professional career[link]

From 1987-1989, Yamanaka was a Resident in orthopedic surgery at the National Osaka Hospital. From 1993-1995, he was a Postdoctoral Fellow at the Gladstone Institute of Cardiovascular Disease, which is affiliated with the University of California, San Francisco. From 1995-1996, he was a staff research investigator at the UCSF-affililated Gladstone Institute of Cardiovascular Disease. From 1996-1999, he was an assistant professor at Osaka City University Medical School. From 1999-2003, he was an associate professor at the Nara Institute of Science and Technology. From 2003-2005, he was a professor at the Nara Institute of Science and Technology. From 2004–2010, Shinya Yamanaka was a professor at the Institute for Frontier Medical Sciences.^[7] Currently Yamanaka is the director and a professor at the Center for iPS Cell Research and Application in Kyoto university, Japan.

In 2006, he and his team generated Induced Pluripotent Stem Cells - pluripotent stem cells from adult mouse fibroblasts. In 2007, he and his team were able to generate Induced Pluripotent Stem Cells from human adult fibroblasts. ^[1][2][3]

Recognitions[link]

In 2007, Shinya Yamanaka was recognized as a "Person Who Mattered" in the Time Person of the Year edition of Time Magazine.^[8] Shinya Yamanaka was also nominated as a 2008 Time 100 Finalist.^[9] In June 2010, Yamanaka was awarded the Kyoto Prize for reprogramming adult skin cells to pluripotential precursors. Yamanaka developed the method as an alternative to embryonic stem cells, thus circumventing an approach in which embryos would be destroyed.^[10] In September 2010 he was awarded the Balzan Prize for his work on biology and stem cells.^[11]

Awards[link]

• 2007 Osaka Science Prize
• 2007 Inoue Prize for Science
• 2007 Asahi Prize
• 2007 Meyenburg Cancer Research Award ^[12][13]
• 2008 Yamazaki-Teiichi Prize in Biological Science & Technology
• 2008 Robert Koch Prize
• 2008 Medals of Honor (Japan) (Medal with Purple Ribbon)
• 2008 Shaw Prize in Life Science & Medicine^[14][15][16]
• 2008 Sankyo Takamine Memorial Award^[17]
• 2009 Lewis S. Rosenstiel Award for Distinguished Work in Basic Medical Research^[18]
• 2009 Gairdner Foundation International Award^[19]
• 2009 Albert Lasker Award for Basic Medical Research
• 2010 March of Dimes Prize in Developmental Biology
• 2010 Kyoto Prize in Biotechnology and medical technology
• 2010 Balzan Prize in biology
• 2010 Person of Cultural Merit
• 2010 BBVA Foundation Frontiers of Knowledge Award in the Biomedicine Category^[20]
• 2011 Albany Medical Center Prize in biomedicine
• 2011 Wolf Prize in medicine
• 2011 King Faisal International Prize
• 2012 Millennium Technology Prize
• 2012 Fellow of the National Academy of Sciences^[4]

References[link]

External links[link]

• The Shinya Yamanaka Lab, CiRA, Kyoto University
• Center for iPS Cell Research and Application, Kyoto University
• Shinya Yamanaka on Charlie Rose
• Fackler, Martin (2007-12-11). "Risk Taking Is in His Genes". The New York Times. http://www.nytimes.com/2007/12/11/science/11prof.html?pagewanted=all. Retrieved 2007-12-11. 
• The Discovery and Future of Induced Pluripotent Stem (iPS)

Rick Perry
Rick Perry in February 2012.
47th Governor of Texas
Incumbent
Assumed office December 21, 2000
Lieutenant	Bill Ratliff David Dewhurst
Preceded by	George W. Bush
39th Lieutenant Governor of Texas
In office January 19, 1999 – December 21, 2000
Governor	George W. Bush
Preceded by	Bob Bullock
Succeeded by	Bill Ratliff
9th Commissioner of Agriculture of Texas
In office January 15, 1991 – January 19, 1999
Governor	Ann Richards George W. Bush
Preceded by	Jim Hightower
Succeeded by	Susan Combs
Member of the House of Representatives of Texas from District 64
In office 1985–1991
Preceded by	Joe Hanna
Succeeded by	John Cook
Personal details
Born	James Richard Perry (1950-03-04) March 4, 1950 (age 62) Paint Creek, Texas
Political party	Republican Party (since 1989) Democratic Party (until 1989)
Spouse(s)	Anita Thigpen
Children	Griffin Sydney
Residence	West Austin, Texas (Temporary residence since 2007, during repairs to the Texas Governor's Mansion)[1]
Alma mater	Texas A&M University (B.S.)
Profession	Military Officer Farmer Politician
Religion	Christianity (evangelical) [2]
Signature
Website	www.governor.state.tx.us
Military service
Service/branch	United States Air Force
Years of service	1972–1977
Rank	Captain
Unit	772nd Tactical Airlift Squadron

	This article is part of a series about Rick Perry	Mitt Romney
2012 Presidential Campaign Governor of Texas Political positions

James Richard "Rick" Perry (born March 4, 1950) is the 47th and current Governor of Texas. Perry, a Republican, was elected Lieutenant Governor of Texas in 1998 and assumed the governorship in December 2000 when then-governor George W. Bush resigned to become President of the United States. Perry was elected to full gubernatorial terms in 2002, 2006 and 2010. With a tenure in office to date of &1000000000000001100000011 years, &10000000000000188000000188 days, Perry is the longest continuously serving current U.S. governor, and the second longest serving current U.S. governor—after Terry Branstad of Iowa. Perry served as Chairman of the Republican Governors Association in 2008 (succeeding Sonny Perdue of Georgia) and again in 2011.^[3] Perry is the longest serving governor in Texas state history. As a result, he is the only governor in modern Texas history to have appointed at least one person to every eligible state office, board, or commission position (as well as to several elected offices to which the governor can appoint someone to fill an unexpired term, such as six of the nine current members of the Texas Supreme Court).

Perry won the Texas 2010 Republican gubernatorial primary election, defeating U.S. Senator Kay Bailey Hutchison and former Wharton County Republican Party Chairwoman and businesswoman Debra Medina.^[4] In the 2010 Texas gubernatorial election, Perry won a third term by defeating former Houston mayor Bill White and Kathie Glass.^[5]

On August 13, 2011, Perry announced in South Carolina that he was running for the Republican nomination for President of the United States in the 2012 presidential election. On January 19, 2012, Perry announced he would be suspending his campaign for the Republican nomination, and endorsed Newt Gingrich.

Early life, education, and military service[link]

A fifth-generation Texan, Perry was born in Paint Creek, about 60 miles (97 km) north of Abilene in West Texas, to ranchers Joseph Ray Perry and the former Amelia June Holt. Perry's ancestry is almost entirely English, dating as far back as the original thirteen colonies. His family has been in Texas since before the Texan Revolution.^[6][7][8]

His father, a Democrat, was a long-time Haskell County commissioner and school board member. Perry has said that his interest in politics probably began in November 1961, when his father took him to the funeral of U.S. Representative Sam Rayburn (D-TX),^[9] who during his long public career served as speaker of the Texas House for a short time at the age of 29 and then later for 17 years as the Speaker of the U.S. House of Representatives.

Perry was in the Boy Scouts (BSA) and earned the rank of Eagle Scout; his son, Griffin, would later become an Eagle Scout as well.^[10][11] The BSA has honored Perry with the Distinguished Eagle Scout Award.^[12]

Perry graduated from Paint Creek High School in 1968. He then attended Texas A&M University, where he was a member of the Corps of Cadets, a member of the Alpha Gamma Rho fraternity, was elected senior class social secretary, and was also elected as one of A&M's five yell leaders.^[13][14] Perry graduated in 1972 with a Bachelor of Science in animal science.^[15][16]

Perry said that the Corps of Cadets gave him the discipline to complete his animal sciences degree and earn a commission in the Air Force. In a 1989 interview he said that "I was probably a bit of a free spirit, not particularly structured real well for life outside of a military regime, I would have not lasted at Texas Tech or the University of Texas. I would have hit the fraternity scene and lasted about one semester."^[15] Perry was a prankster in college: he once placed live chickens in the closet of an upperclassman during Christmas break and used M-80 firecrackers to prank students using the toilet.^[15]

In the early 1970s, Perry interned during several summers with the Southwestern Company, as a door-to-door book salesman. "I count my time working for Dortch Oldham [President of the Southwestern Company] as one of the most important formative experiences of my life," Perry said in 2010. "There is nothing that tests your commitment to a goal like getting a few doors closed in your face." He said that "Mr. Oldham taught legions of young people to communicate quickly, clearly and with passion, a lesson that has served me well in my life since then."^[17]

Upon graduation in 1972, Perry was commissioned in the Air Force and completed pilot training in February 1974. He was then assigned as a C-130 pilot to the 772nd Tactical Airlift Squadron at Dyess Air Force Base. Perry's duties included two-month overseas rotations at RAF Mildenhall in England and Rhein-Main Air Base in Germany. His missions included a 1974 U.S. State Department drought relief effort in Mali, Mauritania and Chad, and two years later, earthquake relief in Guatemala.^[18] He left the Air Force in 1977 with the rank of captain, returned to Texas, and went into business farming cotton with his father.^[19]

Early political career[link]

Texas Legislature[link]

In 1984, Perry was elected to the Texas House of Representatives as a Democrat from district 64, which included his home county of Haskell. He served on the House Appropriations and Calendars committees during his three two-year terms in office. He befriended fellow freshman state representative Lena Guerrero of Austin, a staunch liberal Democrat who endorsed Perry's reelection bid in 2006 on personal, rather than philosophical, grounds.

Perry was part of the "Pit Bulls", a group of Appropriations members who sat on the lower dais in the committee room (or "pit") who pushed for austere state budgets during the 1980s.^[20] At one point, The Dallas Morning News named him one of the ten most effective members of the legislature.^[21]

In 1987, Perry voted for a $5.7 billion tax increase proposed by Republican governor Bill Clements.^[22] Perry supported Al Gore in the 1988 Democratic presidential primaries and worked in an unspecified capacity for Gore's campaign in Texas.^[23] In 1989, Perry announced that he was switching parties, becoming a Republican.^[24]

Agriculture Commissioner[link]

In 1990, as a newly minted Republican, Perry challenged Jim Hightower, the incumbent Democratic Agriculture Commissioner. Hightower had worked on behalf of Jesse Jackson for the Democratic presidential nomination in 1988, while Perry had supported U.S. Senator Al Gore of Tennessee.

During 1990, Hightower's office was embroiled in a FBI investigation into corruption and bribery. Three aides were convicted in 1993 of using public funds for political fundraising, although Hightower himself was not found to be involved in the wrongdoings.^[25] Perry narrowly defeated Hightower in November 1990.

As Agriculture Commissioner, Perry was responsible for promoting the sale of Texas farm produce to other states and foreign nations, and for supervising the calibration of weights and measures, such as gasoline pumps and grocery store scales.^[26]

In April 1993, Perry, while serving as Texas agriculture commissioner, expressed support for the effort to reform the nation's health care, describing it as "most commendable".^[27] The health care plan, first revealed in September, was ultimately unsuccessful due to Republican congressional opposition.^{[28][29][30][31][32]} In 2005, after being questioned on the issue by a potential opponent in the Republican governor primary, Perry said that he expressed his support only in order to get Clinton to pay more attention to rural health care.^[33]

In 1994, Perry was reelected Agriculture Commissioner by a large margin, getting 2,546,287 votes (62 percent) to Democrat Marvin Gregory's 1,479,692 (36 percent). Libertarian Clyde L. Garland received the remaining 85,836 votes (2 percent).^[34] Gregory, a chicken farmer from Sulphur Springs, Texas, was on the Texas Agricultural Finance Authority with Perry in the early nineties, as a Republican. He became a Democrat before running against Perry in 1994.^[35]

Lieutenant Governor[link]

In 1998, Perry chose not to seek a third term as Agriculture Commissioner, running instead for Lieutenant Governor to succeed the retiring Democrat Bob Bullock. During this election, Rick Perry had a notable falling out with his previous top political strategist Karl Rove, which began the much-reported rivalry between the Bush and Perry camps.^[36] Perry polled 1,858,837 votes (50.04 percent) to the 1,790,106 (48.19 percent) cast for Democrat John Sharp of Victoria, who had relinquished the Comptroller's position after two terms to seek the lieutenant governorship. Libertarian Anthony Garcia polled another 65,150 votes (1.75 percent).^[34] Perry thus became the state's first Republican lieutenant governor since Reconstruction, taking office on January 19, 1999.

Governor of Texas[link]

President George W. Bush and Texas Governor Rick Perry shake hands after a question and answer session at the Port Arthur airport. Port Arthur was hit hard by Hurricane Rita.

Perry assumed the office of governor on December 21, 2000, following the resignation of George W. Bush—who was preparing to become President of the United States.^[10] He won the office in his own right in the 2002 gubernatorial election, where he received 57.80% of votes to businessman Tony Sanchez's 39.96%.^[34] He was re-elected in the 2006 gubernatorial election against three major opponents, polling 39% of votes against runner-up Chris Bell with 29.8%. In the 2010 gubernatorial election, Perry became the first Texas governor to be elected to three four-year terms, polling 54.97% of votes to Bill White's 42.48%.

In his three gubernatorial campaigns, Perry received hard-money campaign contributions of $102 million, half of which came from 204 donors.^[37]

In the 2001 legislative session, Perry set a record for his use of the veto, rejecting 82 acts, more than any other governor in any single legislative session in the history of the state since Reconstruction.^{[38] [39][40]}

Fiscal policies[link]

Rick Perry giving a speech at Pioneer Hi-Bred conference

In his presidential campaign, Perry has highlighted the economic success Texas achieved under his governorship, although the true success of his policies has been questioned.^{[41][42][43][44][45][46]} A proclaimed proponent of fiscal conservatism, Perry has often campaigned on job growth and tax issues, such as his opposition to creating a state income tax. Perry refused in 2002 to promise not to raise taxes as governor, and in the following years did propose or approve various tax and debt^[47] increases.^{[48][49][50][51]} In 2009, Perry signed Grover Norquist's pledge to "oppose and veto any and all efforts to increase taxes".

Texas began borrowing money in 2003 to pay for roads and will owe $17.3 billion by the end of 2012, increasing total state debt, from $13.4 billion in 2001 to $37.8 billion in 2011.^[52] The state's public finance authority sold $2 billion in bonds for unemployment benefits, and it's authorized to sell $1.5 billion more if necessary. Texas federal borrowing topped $1.6 billion in October 2010, before the bond sales.^[53] Texans voted November 8, 2011 for a Water Works Bill with an additional $6 billion of debt and against new tax breaks for landowners.^[54] A Republican on the Natural Resources Committee laments “we couldn’t get the votes” which would break Perry's pledge not to raise taxes.^[55]

In 2003, Perry signed legislation that created the Texas Enterprise Fund, which has since given $435 million in grants to businesses. The New York Times reported that many of the companies receiving grants, or their chief executives, have made contributions to Perry's campaigns or to the Republican Governors Association.^[56]

Health[link]

As Governor, Perry has been an outspoken opponent of federal health-care reform proposals and of the Patient Protection and Affordable Care Act, describing the latter as "socialism on American soil".^[57] Perry's focus in Texas has been on tort reform, signing a bill in 2003 which restricted non-economic damages in medical malpractice judgments.^[58] Perry touted this approach in his Presidential campaign, although independent analysts have concluded that it has failed to increase the supply of physicians or limit health-care costs in Texas.^[57][59]

During Perry's governorship, Texas rose from second to first among states with the highest proportion of uninsured residents at 26%, and had the lowest level of access to prenatal care in the U.S.^[57] Perry and the Republican-led state legislature have cut Medicaid spending and made it more difficult to enroll in the program, which currently covers one-third of Texas children.^[57] The cost of caring for uninsured Texans has been borne by those with insurance, leading to substantial rises in insurance premiums and leading Texas to rank next-to-last among states in terms of affordability of health insurance.^[60] The Los Angeles Times wrote that under Perry, "working Texans increasingly have been priced out of private healthcare while the state's safety net has withered."^[60]

Perry's office has argued that Texas represents a model private-sector approach to health-care. His spokeswoman stated that "Texas does provide an adequate safety net to those truly in need... and many individuals simply choose not to purchase healthcare coverage."^[60]

Perry is pro-life and has signed multiple bills creating new rules or restrictions for abortion procedures and funding for such.^[61][62] These bills include a May 2010 law requiring that a sonogram be performed prior to every abortion, and that the practitioner discuss the sonogram images with the patient except in limited cases where the patient may waive the explanation.^[63][64][65] In December 2011, Perry said he had undergone a "transformation" and now opposed all abortions, including in cases of rape and incest. The next day he clarified that he would allow an exception for abortions that would save a mother's life.^[66][67]

In February 2007, Perry issued an executive order mandating that Texas girls receive the HPV vaccine, which protects against some strains of the human papilloma virus, a contributing factor to some forms of cervical cancer.^[68] Following the move, news outlets reported various apparent financial connections between Perry and the vaccine's manufacturer, Merck.^[68][69] Merck's political action committee has contributed $28,500 since 2001 to Perry's campaigns.^[70] The order was criticized by some parents and social conservatives, and a lawsuit was filed later that month.^[71] In May 2007, the Texas Legislature passed a bill undoing the order; Perry did not veto the bill, saying the veto would have been overruled, but blamed lawmakers who supported the bill for the deaths of future cancer victims.^[72]

In 2011, Governor Perry both had adult stem cell surgery himself, in Houston by Dr. Jones, and started "laying the groundwork" for the commercialization of the adult stem cell industry in Texas.^[73][74]

Religion[link]

Perry grew up in the Methodist church, until 2010 when he began attending Lake Hills Church in Austin.^[2] In 2006, Perry stated that he believes in the inerrancy of the Bible and that those who do not accept Jesus as their savior will go to hell. He later clarified, "I don't know that there's any human being that has the ability to interpret what God and his final decision-making is going to be."^[75] In his 2008 book On My Honor, Perry expressed his views on the Establishment Clause and the Free Exercise Clause. "Let's be clear: I don't believe government, which taxes people regardless of their faith, should espouse a specific faith. I also don't think we should allow a small minority of atheists to sanitize our civil dialogue on religious references."^[76] In June 2011, Perry proclaimed August 6 as a Day of Prayer and Fasting, inviting other governors to join him in a prayer meeting hosted by the American Family Association in Houston.^[77][78] The event was criticized as going beyond prayer and fasting to include launching Perry's presidential campaign.^[79]

Perry has called himself "a firm believer in intelligent design as a matter of faith and intellect", and has expressed support for its teaching alongside evolution in Texas schools, but has also said that "educators and local school officials, not the governor, should determine science curriculum."^[80]

Education[link]

Perry has repeatedly attacked the Robin Hood plan to provide court-mandated equitable school financing for all school districts in the state. In 2005, following rejection of Perry's proposal to replace the Robin Hood plan, Perry vetoed all funding for public schools for the 2007–2008 biennium, saying he would not "approve an education budget that shortchanges teacher salary increases, textbooks, education technology, and education reforms. And I cannot let $2 billion sit in some bank account when it can go directly to the classroom."^[81] Following a second rejection of Perry's bill, Perry asked John Sharp to head a task force charged with preparing a bipartisan education plan, which was subsequently adopted.^[82][83]

In 2001, Perry expressed his pride in the enactment of the statute extending in-state tuition to undocumented immigrants who meet Texas' residency requirements. It also required the undocumented students to pledge to apply for permanent residency or citizenship if this became a possibility for them.^[84]

LGBT issues[link]

In 2002, Perry described the Texas same-sex anti-sodomy law as "appropriate".^[85] The United States Supreme Court's civil rights decision in Lawrence v. Texas struck down the statute Perry referred to the following year for violating the 14th Amendment to the U.S. Constitution.

Perry supported the 2005 ballot proposition which amended the Texas constitution by defining marriage as "only a union between a man and a woman" and prohibiting the state from creating or recognizing "any legal status identical or similar to marriage".^[86] In 2011, after New York legalized same-sex marriage, Perry stated that it was their right to do so under the principle of states' rights delineated in the 10th Amendment.^[87] A spokesman later reiterated Perry's support for a federal constitutional amendment banning same-sex marriage, saying that position was not inconsistent since an amendment would require approval by three-fourths of the states.^[88]

In his first book, On My Honor, published in 2008, Perry drew a parallel between homosexuality and alcoholism, writing that he is "no expert on the 'nature versus nurture' debate", but that gays should simply choose abstinence.^[89] During the 2012 presidential campaign, he criticized the repeal of "don't ask, don't tell".^[90]

Crime[link]

Perry's campaigns for lieutenant governor and governor focused on a tough stance on crime. He has supported block grants for crime programs.^[91]

Jeff L. Blackburn, chief counsel of the Innocence Project of Texas, said of Perry that "He has done more good than any other governor we've ever had ... unless, of course, it involves the death penalty. On the death penalty, Rick Perry has a profound mental block".^[92]

In 2007, Perry signed a law ending automatic arrest for marijuana possession.^[93]

Death penalty[link]

The neutrality of this section is disputed. Please see the discussion on the talk page. Please do not remove this message until the dispute is resolved. (November 2011)

Perry supports the death penalty.^[94] In June 2002, he vetoed a ban on the execution of mentally retarded inmates.^[91] As of August 10, 2011, Texas has carried out 234 executions since Rick Perry became governor.^[95] Cases in which Perry has been criticized for his lack of intervention include those of Cameron Todd Willingham, Frances Newton, and Mexican nationals José Medellín and Humberto Leal Garcia.^{[96][97][98][99]}

Perry commuted the death sentence of Kenneth Foster, who was convicted of murder despite evidence that he was only present at the scene of the crime.^[100] Perry also pardoned Tyrone Brown, who was sentenced to life in a Texas maximum security prison for smoking marijuana while on probation.^[101] Perry's actions in both these cases were following the recommendation of the Texas Board of Pardons and Paroles.^[100][101]

Infrastructure[link]

In 2001, Perry proposed the Trans-Texas Corridor, a $145+ billion-dollar project that would build multi-lane highways, rail lines and data lines from Oklahoma to Mexico, and from east to west in southern Texas. Instead of paying for the project with taxes, Perry proposed that it be partially financed, partially built and wholly operated by private contractors, who would subsequently receive all toll proceeds.^[102] All of Perry's gubernatorial opponents opposed the corridor project, as did the 2006 state party platforms of both the Democratic and Republican parties.^[103][104] After much contentious debate between supporters and opponents, an official decision of "no action" was issued by the Federal Highway Administration on July 20, 2010, formally ending the project.

Gun ownership[link]

Perry has an A+ rating from the National Rifle Association.^[105] He possesses a Concealed Handgun License (CHL)^[106] and has signed a number of bills that increased CHL access.^[107]

2012 presidential campaign[link]

Perry campaigning at the Iowa State Fair

Until 2011, Perry persistently denied aspirations to higher office; he was originally included on the 2012 Presidential Straw Poll ballot at the Values Voter Summit in September 2009, but his name was removed at his own request.^[108] In April 2008 while appearing as a guest on CNBC's Kudlow & Company, he specifically stated that he would not agree to serve as Vice President in a McCain administration, stating that he already had "the best job in the world" as governor of Texas. Further, during a Republican gubernatorial debate in January 2010, when asked if he would commit to serving out his term if re-elected, he replied that "the place hasn't been made yet" where he would rather serve than the governor of Texas. In December 2010, when asked if he was a "definite maybe" to run for President in 2012, he replied, "a definite no, brother".^[109]

On May 27, 2011, he said he is "going to think about" running for the 2012 Republican presidential nomination after the close of the Texas legislative session.^[110] Perry said in a response to a question from a reporter, "but I think about a lot of things," he added with a grin.^[111]

On August 11, a Perry spokesman said that he will be running for President in 2012, with plans to announce his formal entry into the race the next day, August 12.^[112][113] Perry himself confirmed it on a visit to KVUE, the ABC affiliate in Austin. As the Associated Press bulletin announcing his entry into the race came across the wire, Perry signed and dated a printed copy of the bulletin.^[114]

On August 13, Perry officially announced that he will be running for president.^[115]

In October 2011, the Washington Post reported that Perry's family leases a hunting camp once called "Niggerhead". According to some local residents interviewed by the Post, the Perrys used the camp for years before painting over a large rock with that name on it, which stands at an entrance to the area, and during this time Perry hosted friends and supporters at the camp.^[116] Perry's campaign disputed the claims, stating that the Perrys painted over the rock almost immediately after acquiring a lease on the property in 1983.^[117][118]

On December 6, Perry released a Presidential campaign video on YouTube called "Strong" discussing his religious beliefs, as well as criticism of Obama's governance.^[119] The video states "there's something wrong in this country when gays can serve openly in the military, but your kids can't openly celebrate Christmas." The video has been largely criticised in the on-line community and has received over 750,000 dislikes on YouTube.^[120]

Perry announced on January 19, 2012, that he would be dropping out of the 2012 Presidential Race, publicly endorsing Newt Gingrich.^[121] However on April 25, 2012, Perry endorsed Mitt Romney after Gingrich announced that he drop out a week later.

Political views[link]

See: Political positions of Rick Perry

Publications[link]

Governor Rick Perry of Texas at a book signing at the Republican Leadership Conference in New Orleans, Louisiana

Rick Perry has written two books:

• On My Honor: Why the American Values of the Boy Scouts are Worth Fighting For was published in February 2008.^[122] In his book, Perry celebrates the positive impact of the organization on the youth of America and criticizes the ACLU for its legal actions against the Boy Scouts of America.^[123]
• His second book, Fed Up! Our Fight to Save America from Washington was published in November 2010.^[124] Perry's second book discusses his support for limited central government.

Perry has also written a lecture about the role of the federal government and the military in disaster management titled Federalizing Disaster Response.^[125]

Personal life[link]

In 1982, Perry married Anita Thigpen, his childhood sweetheart whom he had known since elementary school. They have two grown children, Griffin and Sydney. Anita Perry attended West Texas State University and earned a degree in nursing. She has spearheaded a number of health-related initiatives such as the Anita Thigpen Perry Endowment at the University of Texas Health Science Center at San Antonio, which focuses on nutrition, cardiovascular disease, health education, and early childhood development.^[126] Anita has also helped develop and host the Texas Conference for Women.^[127]

Electoral history[link]

References[link]